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Epidemiology, Molecular Epidemiology and Surveillance of Dengue Viruses in the South Pacific Cao-Lormeau V.M., Aubry M., Dupont-Rouzeyrol M., Roche C.,

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Presentation on theme: "Epidemiology, Molecular Epidemiology and Surveillance of Dengue Viruses in the South Pacific Cao-Lormeau V.M., Aubry M., Dupont-Rouzeyrol M., Roche C.,"— Presentation transcript:

1 Epidemiology, Molecular Epidemiology and Surveillance of Dengue Viruses in the South Pacific Cao-Lormeau V.M., Aubry M., Dupont-Rouzeyrol M., Roche C., O’Connor O., Chanteau S., Aaskov J. Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011

2 I - Introduction South Pacific Ocean Equator

3 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 <300,000 <100,000 Source: f_Oceanian_countries_by_popul ation I - Introduction

4 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 Flaviviridae familly, Flavivirus genus (YFV, WNV, JEV,…) 4 DENV serotypes (DENV-1 to DENV-4) several genotypes (phylogenetic analysis - E gene) Dengue virus (DENV) I - Introduction

5 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 Transmission cycle Within the Pacific region « man-mosquito-man » Mosquito vectors in PICTs Aedes aegypti (Pacific - late 19th) Aedes albopictus (PNG, Solomon, Fiji, Micronesia, Hawaii) Aedes polynesiensis (French Polynesia, Cook, Am Samoa, Samoa, Fiji, Wallis & Futuna, Tokelau, Tuvalu) Aedes cooki (Niue, Tonga) Aedes hebrideus (PNG, Solomon Isl., Vanuatu) Aedes kesseli (Tonga) Aedes pseudoscutellaris (Fiji) Source: Guillaumot L (2005) I - Introduction

6 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 DENV-1 DENV-2 DENV-3 DENV-4 Other DENV-1 DENV-2 DENV-3 DENV-4 Other Sources: Li et al., 2010; INVS-DIT (août 2008, juin 2007) ; Inform’Action N°28, 29, 27, 25, 18; Singh N et al. (2005) II - Epidemiology

7 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, [1]34(1)213(?) 2016 II - Epidemiology

8 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 Long term circulation of a predominant serotype (20/25 yrs) molecular data (E gene) => same genotype (Li et al., 2010; Imrie et al., 2010; Descloux et al., 2009; A-Nuegoonpipat et al., 2004; Chungue et al, 1998, 1997; Lanciotti et al., 1997, 1994) No persistent co-circulation of multiple DENV serotypes endemic virus replaced by the epidemic serotype except (NC, Vanuatu): DENV-1 transmission occurred before, during and after DENV-4 circulated II - Epidemiology

9 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 Epidemic profiles (local specificities) French Polynesia - one phase epidemic that can start at any season - endemic circulation - 5/6 years re-emergence New Caledonia - bi-phasic epidemic (warm/rainy season) - endemic circulation (frequent imported cases of any serotype) - re-emergence II - Epidemiology

10 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011

11 Phylogenetic analysis (E gene) Interests Which region the DENVs circulating in PICTs might have been introduced from ? Are there main routes of PICT-to-PICT distribution of DENVs? Do the molecular data corroborate epidemiological data? DEN-PACSUD project I.Epidemiology, molecular epidemiology and genetic evolution of DENVs in SPICs II. Intra-host genetic diversity of DENV in human and mosquito DENV-4 (1980s-2000yrs), DENV-3 (1990s), DENV-1 (1980s-2000yrs) -> E gene, full genome sequencing III – Molecular Epidemiology

12 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 V I III II IV DENV-1

13 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 V I III II IV DENV-1 Genotype IV DENV-1

14 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 V I III II IV DENV-1 Genotype IV Asia/Southeast Asia ( ) Nauru (1974) Australia (1983) Samoa (2001) Yap (2004) Indian Ocean ( ) 74 DENV-1

15 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 V I III II IV DENV-1 Genotype IV 74 DENV-1 French Polynesia New Caledonia Vanuatu Pacific clade ( ) Hawaii Asia/Southeast Asia ( ) Nauru (1974) Australia (1983) Samoa (2001) Yap (2004) Indian Ocean ( )

16 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 II I III IV DENV-4

17 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 II I III IV DENV-4 Genotype IIa DENV-4

18 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 II I III IV DENV-4 Genotype IIa DENV-4 Asia/Southeast Asia ( )

19 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 II I III IV DENV-4 Genotype IIa French Polynesia New Caledonia Fiji Tonga Solomon Vanuatu Pacific clade ( ) Wallis and Futuna Kiribati Western Samoa DENV-4 Asia/Southeast Asia ( )

20 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 III – Molecular Epidemiology Genetic analysis (nt mutations -> aa substitutions) DENV-1 ( ) Previous results FP strains (Descloux et al., 2009) -> E ( S 222 T ) = 2001 (0%), 2002 (10%), 2003 (67%), (100%) -> E ( K 363 R ) = 7 strains from FP islands, main vector Ae polynesiensis -> other aa substitutions (E, NS4B, NS5) Analysis of complementary strains -> E ( S 222 T ) = FP, NC, Vanuatu (100%) -> full genome sequencing (on going work...)

21 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 III – Molecular Epidemiology Genetic analysis(nt mutations -> aa substitutions) DENV-4 ( ) -> E (T 365 I) = Pacific clade (Li et al., 2010; Cao-Lormeau et al., under revision) -> other substitutions on E = specific to strains from Tonga, Kiribati, WF (but only one representative) -> full genome sequencing (on going work...)

22 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 Weekly epidemiological reports Regional syndromic surveillance net-work (PPHSN) Physicians sentinel network Laboratory surveillance Real time RT-PCR (Taqman multiplex) type specific NS1 diagnostic test (ELISA) IgM diagnostic test Vector control unit Local surveillance (ex. FP & NC) IV - Surveillance Pilot study on the use of filter papers for the surveillance of the DENV serotypes circulating in PICTs (ILM, IPNC, QUT, SPC)

23 Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011 Institut Pasteur de Nouvelle Calédonie (NC) Pr Suzanne Chanteau Dr Myrielle Dupont-Rouzeyrol Mrs Olivia O’Connor Queensland University of technology (Australia) Pr John Aaskov Direction de la Santé (FP) Dr Henri-Pierre Mallet Mrs Elise Daudens Medical analysis lab, Sia hospital (Wallis) Dr Jean François Yvon Université de la Polynésie française (FP) Pr Marcel Le Pennec Institut Louis Malardé (FP) Research lab. in medical virology Mrs Claudine Roche Mrs Maite Aubry Mr Jérôme Viallon Dr Vaea Richard Medical analysis lab. Dr Didier Musso Dr Stéphane Lastère Mrs Anita Teissier Research lab. In medical entomology Dr Hervé Bossin Mr Jérôme Marie Acknowledgements Secretariat of the Pacific Community (NC) Mrs Salanieta Elbourne Grants: Fonds Pacifique (2008, 2009, 2010); Agence Nationale pour la Recherche (ANR 09 MIEN ) Asia-Pacific Institute of Tropical Medicine and Infectious Diseases (Hawaii) Pr Duane J Gubler Dr Shannon Bennett

24 Mauruuru! Gracias! Thank you! Institut Louis Malardé, Papeete, French Polynesia ECTMIH Barcelona - October 4, 2011


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