Presentation is loading. Please wait.

Presentation is loading. Please wait.

단독생활형 사냥벌의 생활특성과 독액의 산업적 이용

Similar presentations


Presentation on theme: "단독생활형 사냥벌의 생활특성과 독액의 산업적 이용"— Presentation transcript:

1 단독생활형 사냥벌의 생활특성과 독액의 산업적 이용
Solitary Wasp Venom 단독생활형 사냥벌의 생활특성과 독액의 산업적 이용 곤충분자생물학 및 독성학 연구실 백 지 형

2 Solitary Wasp Venom 1. Introduction
2. Taxonomy and Ecology of Solitary Wasp 3. Venom of Solitary Wasp 4. Industrial and Medical Application

3 Ovipositor and Sting

4 Acute Management Antihistamines (oral)
Severe Moderate Mild Systemic Adrenaline (intramuscular) Chlorpheniramine (intramuscular or slow intravenous) Hydrocortisone (intramuscular or slow intravenous) Antihistamines (intramuscular) Hydrocortisone (intramuscular) Inhaled β2 agonist (if asthma) Inhaled adrenaline (if laryngeal oedema) Antihistamines (oral or intramuscular) Antihistamines (oral) Local Treatment Type of reaction

5 Solitary Wasp Solitary Wasp Solitary Subsocial Predatory Parasitic
Pollination Killer Collector

6 Eumenidae 호리병벌과 Anterhynchium flavomarginatum Smith 10-21 mm

7 Eumenidae 호리병벌과 Orancistrocerus drewseni Saussure, 1857

8 Subsocial Eumenidae 줄무늬감탕벌
       줄무늬감탕벌      벌목[膜翅目] 호리병벌과의 곤충. 학명 : Orancistrocerus drewseni 분류 : 벌목[膜翅目] 호리병벌과 분포지역 : 한국 ·일본 ·중국 크기 : 몸길이 약 18mm 몸길이 약 18 mm이다. 몸 빛깔은 흑색인데 등황색의 점무늬가 있다. 몸 전체에 대형의 점각이 촘촘히 있고 가운뎃가슴등판과 작은방패판에 불규칙한 세로주름이 많다. 암컷 성충은 진흙으로 물건의 사이에 육아실을 만드는데 미완성 단계에서는 출입구를 연통같이 만들지만 나중에 제거한다. 그리고 육아실 위에 진흙을 덧칠하여 보강한다. 독방은 처음 만든 속에 있는 것이 크고 여기서 자란 벌은 반드시 암컷이 된다. 유충의 먹이로는 잎말이나방류 ·명나방류 ·밤나방류의 유충을 마비시켜 저장한다. 6∼9월에 많이 활동한다. 한국 ·일본 ·중국에 분포한다.

9 Solitary Eumenidae 한국황슭감탕벌 koreanum Sk. Yamane
       한국황슭감탕벌      벌목[膜翅目] 호리병벌과의 곤충. 학명 : Anterhychium flavomarginatum koreanum Sk. Yamane 분류 : 벌목[膜翅目] 호리병벌과 크기 : 몸길이 약 18mm 암컷은 흑색이다. 머리와 가슴에 점각이 밀포되었고 암컷의 머리방패는 길이가 폭보다 길고, 암수 모두에서 이마방패 정단부의 함입은 좁고 얕다. 머리방패는 넓게 밝은 노랑색이다. 더듬이 사이의 한 개의 작은 점과 자루마디 앞면은 유백색이다. 앞가슴의 변두리에는 중단된 황색줄이 있고 어깨판에는 적갈색 점이 있고 날개는 흐렸으며 그 앞슭은 암색이다.기본적으로 제 1-2배마디 등판 뒷슭에 노란색띠무늬가 있다. 한국에는 원아종과 한국황슭감탕벌(ssp. koreanus)의 두 아종이 있다. 몸길이는 15-18㎜정도이다.

10 Subsocial vs. Solitary Wasp
Subsocail Wasp Begins provisioning just before egg hatching Continues until the larva grows to instars 3-5, for about one week. Solitary Wasp Lays an egg and then provisions for 1-2 days After sealing with a mud wall, starts the second oviposition

11 Subsocial vs. Solitary Wasp
Emerge from mid to late Jun Oviposition begins ca. 4 days after emergence until late Aug 2nd generation emerge in Aug, oviposit until mid Oct 4. Prepupae diapause in cells Females: 1st (2-2.5 months) 2nd (1-1.5 months) 6. One female lays 6-8 eggs Emerge from Jun to Jul 2. Mate soon after emergency 3. Oviposit from mid Aug to late Oct 4. Prepupae diapause in cells 5. Females: 2-3 months 6. One female lays eggs

12 Subsocial vs. Solitary Wasp
Life Cycle Egg 3-4 days Larva 5-9 days Overwintering Prepupa 8-9 months Pupa 5-10 days Solitary Prepupa 20-30 days Pupa 5-10 days Egg 3-4 days Larva 5-9 days Subsocial

13 Venom of Wasp Vespoidea Masaridae Vespidae – Venom studies Eumenidae
Feed on plants Feed their offspring with nectar and pollen Vespidae – Venom studies The true vespid wasps kill their prey Feed their offspring with flesh of the killed prey Eumenidae Long-term, non-lethal paralysis

14 Components of Venom Honey Bee Hornet, Yellow jacket and Wasp
Peptides and Amines - Mellittin - Apamin - MCD-Peptide - Histamin - Dopamin - Serotonin - Norepinphrin Enzymes - Phospholipase A - Hyaluronidase - Acidic phosphatase - 58 enzymes Hornet, Yellow jacket and Wasp Mellittin-like peptides Bombolitin: Bumblebee venom (17 amino acid amphiphilic alpha-helix) Mastoparan: Wasp venom (14 amino acid amphiphilic alpha-helix) crabrolin: Hornet venom (13 amino acid amphiphilic alpha-helix) Antigen 5

15 Clinical Effects of Venom

16 Mellitin의 작용기작 Mellitin is the chief toxin with a helical structure by virtue of which it can form pores within cell membranes causing structural disruption. Toxin dispersion is facilitated by hyaluronidase which is known to increase capillary permeability. Corneal avascularity results in a high concentration of toxins with delayed washout

17 Venom of Vespid Active Amines
Serotonin, Histamine, Tyramine and Catecholamine Major pain-producing principles Polyamines (putrescine, spermidine and spermine) - spider and scorpion venom Kinins (혈관 확장, 평활근의 수축 또는 저혈압 등을 일으키는 내외성 펩티드) Pain-producing wasp peptides Bradykinin-like peptides (snake) Release histamine from mast cells Mastoparan Mast cell degranulating peptides Granule- and Histamine-releasing peptides Allergens Acid phosphatas, Hyaluronidase and Phospholipase Nonenzymatic protein (Antigens 5) – The most active allergen

18 Venom of Vespid Neurotoxins Mandaratoxin (Vespid neurotoxin) Toxin
signature Amino acid a.a Origin Action philanthotoxin Philanthus triangulum inhibition of neuromuscular transmission by block of the postsynaptic glutamate receptors. alpha-pompilidotoxin α-PMTX RIKIGLFQDLSKL 13 Anoplius samariensis Slow or block conformational change of the Na+ channels beta-pompilidotoxin β-PMTX RIKIGLFQDLSRL Slows the inactivation of Na+ channels on rat hippocampal CA1 interneurons bradykinins BK RPPGFSPFR 9 Megacampsomerus prismatica nicotinic acetylcholine receptor blocking threonine6-bradykinin Thr6-BK RPPGFTPFR protopolybia-MP I Mastoparan INWLKLGKKVSAIL 14 Protopolybia exigua hemolytic mastoparan protopolybia-MP II INWKAIIEAAKQAL non-hemolytic mast cell degranulator toxin protopolybia-MP III mastoparan INWLKLGKAVIDAL anoplin GLLKRIKTLL 10 antimicrobial activity crabrolin FLPLILRKIVTAL Vespa crabro

19 Antibiotics Philanthus triangulum, Fabricius 1775
(Hymenoptera: Specidae), Bee-Wolf

20 Kaltenpoth et al., 2005. Current Biology, 15, pp. 475-479
Secretion of White Substance by a Beewolf Female in the Brood Cell After entering the brood cell, the beewolf female moves its body laterally and secretes massive amounts of white substance from its specialized antennal glands. The particles are smeared to the ceiling of the cell. The whole process takes about 30 s. Uptake of White Substance by a Beewolf Larva in the Brood Cell The larva is apparently searching for the white substance and ingests parts of it.

21 Antibiotics

22 Application of Venom New Insecticide Drug
Quistad, G.B., Reuter, C.G., Skinner, W.S., Dennis, P.A., Suwanrumpha, S., Fu, E.W., Paralytic and insecticidal toxins from the funnel web spider, iHololena curta. iToxicon 29, Bloomquist, J.R., Kinne, L.P., Deutsch, V., Simpson, S.F., Mode of action of an insecticidal peptide toxin from the venom of a weaving spider (iDiguetia canities). Toxicon 34, Drug Harvey, A.L., Bradley, K.N., Cochran, S.A., Rowan, E.G., Pratt, J.A., Quillfeldt, J.A., Jerusalinsky, D.A., What can toxins tell us for drug discovery? Toxicon 36, Heading, C.E., Conus peptides and neuroprotection. Curr. Opin. Invest. Drugs 3, Molecular Mechanism of Neurotransmission

23 Related Documents Wasp Venom New Insecticides Pain Killer Anesthetic
Antibiotics Medical Industrial Immuno-therapy


Download ppt "단독생활형 사냥벌의 생활특성과 독액의 산업적 이용"

Similar presentations


Ads by Google