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Solitary Wasp Venom 단독생활형 사냥벌의 생활특성과 독액의 산업적 이용 곤충분자생물학 및 독성학 연구실 백 지 형.

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Presentation on theme: "Solitary Wasp Venom 단독생활형 사냥벌의 생활특성과 독액의 산업적 이용 곤충분자생물학 및 독성학 연구실 백 지 형."— Presentation transcript:

1 Solitary Wasp Venom 단독생활형 사냥벌의 생활특성과 독액의 산업적 이용 곤충분자생물학 및 독성학 연구실 백 지 형

2 Solitary Wasp Venom 2. Taxonomy and Ecology of Solitary Wasp 2. Taxonomy and Ecology of Solitary Wasp 3. Venom of Solitary Wasp 3. Venom of Solitary Wasp 4. Industrial and Medical Application 4. Industrial and Medical Application 1. Introduction 1. Introduction

3 Ovipositor and Sting

4 Acute Management Severe Moderate Mild Systemic Adrenaline (intramuscular) Chlorpheniramine (intramuscular or slow intravenous) Hydrocortisone (intramuscular or slow intravenous) Antihistamines (intramuscular) Hydrocortisone (intramuscular) Inhaled β2 agonist (if asthma) Inhaled adrenaline (if laryngeal oedema) Antihistamines (oral or intramuscular) Antihistamines (oral) Local TreatmentType of reaction

5 Solitary Wasp Solitary Subsocial Predatory Parasitic Pollination Killer Collector

6 Anterhynchium flavomarginatum Smith mm Eumenidae 호리병벌과

7 Orancistrocerus drewseni Saussure, 1857

8 줄무늬감탕벌 벌목 [ 膜翅目 ] 호리병벌과의 곤충. 학명 : Orancistrocerus drewseni 분류 : 벌목 [ 膜翅目 ] 호리병벌과 분포지역 : 한국 · 일본 · 중국 크기 : 몸길이 약 18mm 몸길이 약 18 mm 이다. 몸 빛깔은 흑색인데 등황색의 점무늬가 있다. 몸 전체에 대 형의 점각이 촘촘히 있고 가운뎃가슴등판과 작은방패판에 불규칙한 세로주름이 많 다. 암컷 성충은 진흙으로 물건의 사이에 육아실을 만드는데 미완성 단계에서는 출 입구를 연통같이 만들지만 나중에 제거한다. 그리고 육아실 위에 진흙을 덧칠하여 보강한다. 독방은 처음 만든 속에 있는 것이 크고 여기서 자란 벌은 반드시 암컷이 된다. 유충의 먹이로는 잎말이나방류 · 명나방류 · 밤나방류의 유충을 마비시켜 저장 한다. 6 ∼ 9 월에 많이 활동한다. 한국 · 일본 · 중국에 분포한다. Subsocial Eumenidae

9 한국황슭감탕벌 벌목 [ 膜翅目 ] 호리병벌과의 곤충. 학명 : Anterhychium flavomarginatum koreanum Sk. Yamane 분류 : 벌목 [ 膜翅目 ] 호리병벌과 크기 : 몸길이 약 18mm 암컷은 흑색이다. 머리와 가슴에 점각이 밀포되었고 암컷의 머리방패는 길이가 폭 보다 길고, 암수 모두에서 이마방패 정단부의 함입은 좁고 얕다. 머리방패는 넓게 밝은 노랑색이다. 더듬이 사이의 한 개의 작은 점과 자루마디 앞면은 유백색이다. 앞가슴의 변두리에는 중단된 황색줄이 있고 어깨판에는 적갈색 점이 있고 날개는 흐렸으며 그 앞슭은 암색이다. 기본적으로 제 1-2 배마디 등판 뒷슭에 노란색띠무늬 가 있다. 한국에는 원아종과 한국황슭감탕벌 (ssp. koreanus) 의 두 아종이 있다. 몸 길이는 ㎜정도이다. Solitary Eumenidae

10 Subsocail Wasp - Begins provisioning just before egg hatching - Continues until the larva grows to instars 3-5, for about one week. Solitary Wasp - Lays an egg and then provisions for 1-2 days - After sealing with a mud wall, starts the second oviposition Subsocial vs. Solitary Wasp

11 Solitary - 황슭감탕벌 Solitary - 황슭감탕벌 1.Emerge from mid to late Jun 2.Oviposition begins ca. 4 days after emergence until late Aug after emergence until late Aug 3.2 nd generation emerge in Aug, oviposit until mid Oct oviposit until mid Oct 4. Prepupae diapause in cells 5.Females: 1 st (2-2.5 months) 2 nd (1-1.5 months) 2 nd (1-1.5 months) 6. One female lays 6-8 eggs Subsocial - 줄무늬감탕벌 Subsocial - 줄무늬감탕벌 1.Emerge from Jun to Jul 2. Mate soon after emergency 3. Oviposit from mid Aug to late Oct 4. Prepupae diapause in cells 5. Females: 2-3 months 6. One female lays eggs

12 Egg 3-4 days Egg 3-4 days Larva 5-9 days Larva 5-9 days Overwintering Prepupa 8-9 months Overwintering Prepupa 8-9 months Pupa 5-10 days Pupa 5-10 days Prepupa days Prepupa days Egg 3-4 days Egg 3-4 days Larva 5-9 days Larva 5-9 days Pupa 5-10 days Pupa 5-10 days Solitary Subsocial Subsocial vs. Solitary Wasp Life Cycle

13 Venom of Wasp VespoideaMasaridae Feed on plants Feed their offspring with nectar and pollen Vespidae – Venom studies The true vespid wasps kill their prey Feed their offspring with flesh of the killed preyEumenidae Long-term, non-lethal paralysis

14 Components of Venom Peptides and Amines - Mellittin - Apamin - MCD-Peptide - Histamin - Dopamin - Serotonin - Norepinphrin Enzymes Enzymes - Phospholipase A - Hyaluronidase - Acidic phosphatase - 58 enzymes - Mellittin-like peptides Bombolitin: Bumblebee venom (17 amino acid amphiphilic alpha-helix) Mastoparan: Wasp venom (14 amino acid amphiphilic alpha-helix) crabrolin: Hornet venom (13 amino acid amphiphilic alpha-helix) - Antigen 5 Honey Bee Hornet, Yellow jacket and Wasp

15 Clinical Effects of Venom

16 Mellitin 의 작용기작 Mellitin is the chief toxin with a helical structure by virtue of which it can form pores within cell membranes causing structural disruption. Toxin dispersion is facilitated by hyaluronidase which is known to increase capillary permeability. Corneal avascularity results in a high concentration of toxins with delayed washout

17 Venom of Vespid Active Amines Serotonin, Histamine, Tyramine and Catecholamine Major pain-producing principles Polyamines (putrescine, spermidine and spermine) - spider and scorpion venom Kinins ( 혈관 확장, 평활근의 수축 또는 저혈압 등을 일으키는 내외성 펩티드 ) Pain-producing wasp peptides Bradykinin-like peptides (snake) Release histamine from mast cellsMastoparan Mast cell degranulating peptides Granule- and Histamine-releasing peptidesAllergens Acid phosphatas, Hyaluronidase and Phospholipase Nonenzymatic protein (Antigens 5) – The most active allergen

18 Venom of Vespid Neurotoxins Mandaratoxin (Vespid neurotoxin) ToxinsignatureAmino acida.aOriginAction philanthotoxinPhilanthus triangulum inhibition of neuromuscular transmission by block of the postsynaptic glutamate receptors. alpha-pompilidotoxinα-PMTXRIKIGLFQDLSKL13Anoplius samariensis Slow or block conformational change of the Na+ channels beta-pompilidotoxinβ-PMTXRIKIGLFQDLSRL13Anoplius samariensis Slows the inactivation of Na+ channels on rat hippocampal CA1 interneurons bradykininsBKRPPGFSPFR9Megacampsomerus prismatica nicotinic acetylcholine receptor blocking threonine6-bradykininThr6-BKRPPGFTPFR9Megacampsomerus prismatica nicotinic acetylcholine receptor blocking protopolybia-MP IMastoparanINWLKLGKKVSAIL14Protopolybia exiguahemolytic mastoparan protopolybia-MP IIMastoparanINWKAIIEAAKQAL14Protopolybia exigua non-hemolytic mast cell degranulator toxin protopolybia-MP IIImastoparanINWLKLGKAVIDAL14Protopolybia exigua non-hemolytic mast cell degranulator toxin anoplinGLLKRIKTLL10Anoplius samariensisantimicrobial activity crabrolinFLPLILRKIVTAL13Vespa crabro

19 Philanthus triangulum, Fabricius 1775 (Hymenoptera: Specidae), Bee-Wolf Antibiotics

20 Secretion of White Substance by a Beewolf Female in the Brood Cell After entering the brood cell, the beewolf female moves its body laterally and secretes massive amounts of white substance from its specialized antennal glands. The particles are smeared to the ceiling of the cell. The whole process takes about 30 s. Uptake of White Substance by a Beewolf Larva in the Brood Cell The larva is apparently searching for the white substance and ingests parts of it. Kaltenpoth et al., Current Biology, 15, pp

21 Antibiotics

22 Application of Venom New Insecticide Quistad, G.B., Reuter, C.G., Skinner, W.S., Dennis, P.A., Suwanrumpha, S., Fu, E.W., Paralytic and insecticidal toxins from the funnel web spider, iHololena curta. iToxicon 29, Bloomquist, J.R., Kinne, L.P., Deutsch, V., Simpson, S.F., Mode of action of an insecticidal peptide toxin from the venom of a weaving spider (iDiguetia canities). Toxicon 34, Drug Harvey, A.L., Bradley, K.N., Cochran, S.A., Rowan, E.G., Pratt, J.A., Quillfeldt, J.A., Jerusalinsky, D.A., What can toxins tell us for drug discovery? Toxicon 36, Heading, C.E., Conus peptides and neuroprotection. Curr. Opin. Invest. Drugs 3, Molecular Mechanism of Neurotransmission

23 Related Documents New Insecticides New Insecticides Pain Killer Anesthetic Pain Killer Anesthetic Antibiotics -Medical - Industrial Antibiotics -Medical - IndustrialWaspVenom Immuno- therapy


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