Presentation is loading. Please wait.

Presentation is loading. Please wait.

Is the methoprene tolerant gene transcribed during mosquito metamorphosis? Jayson Jones Mentor: Dr. James Nishiura Brooklyn College Harlem Children Society.

Similar presentations


Presentation on theme: "Is the methoprene tolerant gene transcribed during mosquito metamorphosis? Jayson Jones Mentor: Dr. James Nishiura Brooklyn College Harlem Children Society."— Presentation transcript:

1 Is the methoprene tolerant gene transcribed during mosquito metamorphosis? Jayson Jones Mentor: Dr. James Nishiura Brooklyn College Harlem Children Society 2006 http://entomology.ucdavis.edu/.../1-A%20gambiae-1.jpg

2 Malaria  An infection caused by the protozoa of the genus Plasmodium.  Parasites invade the red blood cells causing them to burst.  Symptoms include chills, fever, and flu like symptoms.

3 Malaria in the U.S. FMalaria has been eradicated since 1951. FCases of malaria in the U.S. are due to traveling. F1,324 cases of malaria, with four deaths, occurred in 2004. Four of those deaths were not due to travel. FMalaria has been eradicated since 1951. FCases of malaria in the U.S. are due to traveling. F1,324 cases of malaria, with four deaths, occurred in 2004. Four of those deaths were not due to travel.

4

5 Malaria Worldwide  Forty percent of the population in the world live in areas where malaria is transmitted. (South America, Africa, etc.)  300-500 million cases occur each year.  About 1.5-2.7 million people die due to malaria each year, and most of them are African children.  About 3000 children under the age of five contract malaria each day.  Forty percent of the population in the world live in areas where malaria is transmitted. (South America, Africa, etc.)  300-500 million cases occur each year.  About 1.5-2.7 million people die due to malaria each year, and most of them are African children.  About 3000 children under the age of five contract malaria each day.

6 Anopheles Gambiae http://www.micromacro.co.uk/science/large_images/sc3025.jpg

7 Aedes Aegypti

8

9 Metamorphosis Four stages of development in larva, called instars Metamorphosis starts in the fourth instar From larva-pupa-mosquito

10 Juvenile and Ecdysone hormones  Juvenile and ecdysone hormones help mosquitoes develop  Juvenile Hormone maintains the larval stage of development.  Ecdysone causes molting  Juvenile Hormone and Ecdysone Hormone work together  Juvenile and ecdysone hormones help mosquitoes develop  Juvenile Hormone maintains the larval stage of development.  Ecdysone causes molting  Juvenile Hormone and Ecdysone Hormone work together

11 Juvenile Hormone / JH0 C 19 H 32 O 3 / JH I C 18 H 30 O 3 / JH II C 17 H 28 O 3 / JH III C 16 H 26 O 3 (most commonly found) / JHB3 C 16 H 26 O 4 / JH0 C 19 H 32 O 3 / JH I C 18 H 30 O 3 / JH II C 17 H 28 O 3 / JH III C 16 H 26 O 3 (most commonly found) / JHB3 C 16 H 26 O 4

12 Methoprene Juvenile Hormone analog Placed in water where egg rafts are located IGR-insect growth regulator C 19 H 34 O 3 Juvenile Hormone analog Placed in water where egg rafts are located IGR-insect growth regulator C 19 H 34 O 3

13 Methoprene Tolerant Gene Resists the effects of Methoprene No one knows exactly when it is transcribed and no one has studied its transcription in mosquitoes before.

14 WHAT IS THE POINT? Learning if the methoprene tolerant gene is transcribed during metamorphosis will lead to future studies on the mysterious gene and hopefully future discoveries relating to mosquitoes and malaria prevention.

15 Materials Tweezers Test tubes PBS (cleaning solution) TRI-Reagent (denaturing agent) Slides Microscope pipets Tweezers Test tubes PBS (cleaning solution) TRI-Reagent (denaturing agent) Slides Microscope pipets

16 Methods and Procedure  Separate mosquito larva of all stages into individual test tubes. (640 in total)  Collect larva of different ages  Isolate their midguts through dissection.  Extract RNA from the midguts  Run reverse transcriptase  Real Time PCR

17 Isolating the midguts  5 midguts at a time-10 midguts/test tube  Cut at third abdominal region  Cut the head off  As the midgut comes shooting out make a small incision and remove the peritrophic membrane.  Pull the midgut out and preserve in TRI- Reagent and stored in the freezer  Dissections every 3hrs http://www.landcareresearch.co.nz/r esearch/biodiversity/invertebratespr og/invertid/images/Mosquito_Larva 1

18 Results No results yet. We hope that the methoprene tolerant gene is transcribed during metamorphosis because it would be one step closer in discovering how to prevent malaria. No results yet. We hope that the methoprene tolerant gene is transcribed during metamorphosis because it would be one step closer in discovering how to prevent malaria.

19 References FFlatt, Thomas, Tadeusz J. Kawecki. “Pleiotropic effects of methoprene tolerant (Met), a gene involved in juvenile hormone, on life history traits in Drosphilia melanogastar.” Genetica 122 (2004): 141-160. FMiura, Ken. Masahito Oda, Sumiko Makita, Yasuo Chinzei. “Characterization of the Drosphila Methoprene tolerant gene product.” FEBS Journal 272 (2005). FWilson, Thomas G, Yoram Yerushalmi,, David M. Donnell, Linda L. Restifo. “Interaction between Hormonal Signaling pathways in Drosphila melanogaster as Revealed by Genetic Interaction between Methoprene Tolerant and Broad Complex.” Genetics Society of America (2006). FMinkoff, Charles III., Thomas G. Wilson. “The Competetive ability and Fitness Components of the Methoprene Tolerant (Met) Drosphila Mutant Resistant to Juvenile Hormone Analog Insecticides.” Genetics Society of America (1992). F“Plasmodium falciparum genome projects.” Wellcome Trust Sanger Institute. 21 Aug. 2006..http://www.sanger.ac.uk/Projects/P_falciparum F“Latest United States Malaria Disease Surveillance Report-A need for Continuing Vigilance.” Centers for Disease Control and Prevention. May 26th, 2006. 21 Aug 2006..http://www.cdc.gov/malaria FFlatt, Thomas, Tadeusz J. Kawecki. “Pleiotropic effects of methoprene tolerant (Met), a gene involved in juvenile hormone, on life history traits in Drosphilia melanogastar.” Genetica 122 (2004): 141-160. FMiura, Ken. Masahito Oda, Sumiko Makita, Yasuo Chinzei. “Characterization of the Drosphila Methoprene tolerant gene product.” FEBS Journal 272 (2005). FWilson, Thomas G, Yoram Yerushalmi,, David M. Donnell, Linda L. Restifo. “Interaction between Hormonal Signaling pathways in Drosphila melanogaster as Revealed by Genetic Interaction between Methoprene Tolerant and Broad Complex.” Genetics Society of America (2006). FMinkoff, Charles III., Thomas G. Wilson. “The Competetive ability and Fitness Components of the Methoprene Tolerant (Met) Drosphila Mutant Resistant to Juvenile Hormone Analog Insecticides.” Genetics Society of America (1992). F“Plasmodium falciparum genome projects.” Wellcome Trust Sanger Institute. 21 Aug. 2006..http://www.sanger.ac.uk/Projects/P_falciparum F“Latest United States Malaria Disease Surveillance Report-A need for Continuing Vigilance.” Centers for Disease Control and Prevention. May 26th, 2006. 21 Aug 2006..http://www.cdc.gov/malaria

20 Acknowledgements Dr. James Nishiura Dr. Sat Bhattacharya Harlem Children Society Memorial Sloan Kettering Cancer Center Ms. Belynda Rivers Mr. Greg Johnson Ms. Dacota Stewart Dick Mr. David Connelly Dr. McEntee Ms. Kathryn Ray Gateway Institute Brooklyn College


Download ppt "Is the methoprene tolerant gene transcribed during mosquito metamorphosis? Jayson Jones Mentor: Dr. James Nishiura Brooklyn College Harlem Children Society."

Similar presentations


Ads by Google