2Mycotoxins are natural chemicals produced by certain fungi(molds) which occur as contaminants of some food crops, either in the field or during post-harvest storage. Cereals, nuts, fruits and foods derived from these crops are the most likely to contain mycotoxins. mycotoxins are toxic to humans and animalsThree types of fungi are the major producers of mycotoxins – Fusarium, Pencillium, Aspergillus. Within each type of fungus, particular species may be mycotoxigenicor mycotoxin-producing
3While hundreds of mycotoxins have been identified from a very large number of fungi, only mycotoxins have been associated with potential toxicity to animals and humansAspergilluspenicilliumfusarium
4Types of mycotoxinsThere are over 300 mycotoxins but the commonly occurring ones in food and feed. about 20 mycotoxins occur in food at levels and frequency to be of food safety concern.Common Members of the Mycotoxin FamilyAflatoxins, Fumonisin, Ochratoxins, Patulin, Zearalenone, FumonisinsTrichothecenes(Type A: DAS, T-2), Patulin, Moniliform, SterigmatocystinCyclopiazonic acid, Kojic acid, Maltoryzine, ß-nitropropionic acid Aspergillic acid, Penicillic acid, Roquefortine C
6mycotoxin occurrence in foods Mycotoxin is produced by a fungus under special conditions of moisture and temperature in :1- Mould damaged foodstuffs including agricultural products like cereals, oilseeds, fruits and consumer foods such as peanut butter2-Residues in animal tissues and products e.g. milk, meat, dairy produce3-Mould ripened foods e.g. cheeses, meats4-Fermentation products e.g. microbial proteins and enzymes.Juices e.g. apple juice
8AflatoxinsToxins produced by the fungi Aspergillus flavus and Aspergillus parasiticusFour primary aflatoxins, named B1, B2, G1 and G2 plus two additional metabolic products, M1 and M2. Aflatoxins occur in nuts, cereals and rice underconditions of high humidity and temperature andpresent a risk to human and animal health
9Aflatoxins: Human, Animal, and Environmental Interactions Fungal growth / aflatoxin productionContamination of human & animal foodAnimal consumptionHuman consumptionEnvironment: extreme drought, moisture, heat, compromised plantsBreast milkIn utero
10Mechanism of toxicologic damage Because of aflatoxins’ common occurrence in feedstuffs, feeds and milk products. Although the oral route is the main contamination means, inhalation may also occur as a result of people or animals being exposed to the grains’ dust. After respiratory exposure, AfB1 may appear in the blood more quickly than after oral exposure. Nevertheless,. Following ingestion, aflatoxin B1 is efficiently absorbed in the intestinal tract, of which the duodenum appears to be the major site of absorption. Due to the particle’s low molecular weight, the main mechanism of absorption of mycotoxin, as suggested by several authors, is passive diffusion, in which no efflux pumps or transporters are involved.,The main metabolizing organ for aflatoxin is the liveaflatoxins exert their toxic effects.
11Mechanism of toxicologic damage At first stage, aflatoxin is oxidized into several hydroxylated metabolitesreduction to aflatoxicol and hydroxylation to epoxide (acutely toxic, mutagenic, and carcinogenic)Aflatoxin binds to guanine in deoxyribonucleic acid (DNA), inhibiting the segnal for the formation of messenger ribonuceic acid (RNA), interruption of protein synthesis leads to deficies of structural protein, enzymes,lipid acceptor protein, the long term effects of impaired protein synthesis include hepatic steatosis and avariety of metabolic and fanction derangments
12Effect of aflatoxin on health * Teratogenic* ImmunosuppressiveMany times enhanced in the presence of hepatitis B and C viruses.* Carcinogenic
13Aflatoxin Symptoms 1- Onset of high fever 2- Progressive jaundice 3- Liver damage4- Liver cancer5- Abdominal Pain6- Vomiting7- Convulsions8- Edema and Pulmonary Edema9- Hemorrhaging10- Disruption of food digestion, absorption or metabolism
14T-2 mycotoxinT-2 mycotoxins belong to the ‘trichothecene' group, and are mainly produced by Fusarium type moulds. These moulds develop on cereals in the fields, especially when the weather is wet and relatively cold during flowering. Although it can occur naturally from moldy, improperly stored gains.It cause1- Hematopoietic effects,2- Immunosuppression,3- Gastro-intestinal effects,4- Neurotoxic effects (restlessness; lack of reflexes; nervous syndrome),5- Dermotoxicity(necrosis),
15Absorption and Mechanism of action In general, absorption of T2 occurs very rapidly within the digestive tract and is widely distributed in many tissues and organs. Currently, there is no evidence for accumulation in tissues or transmission to milk or eggs.T2 toxin are inhibition of protein synthesis, thought to be the most important effect, inhibition of DNA synthesis, impairment of ribosome function, T2 bind to active polysomes and ribosomes inhibition of mitochondrial protein synthesis, immunosuppression, allowing secondary and opportunistic bacterial infections and possibly delayed hypersensitivity
16Breathing T2 mycotoxin in an aerosol form * Ingestion possible methods of exposure* InhalationBreathing T2 mycotoxin in an aerosol form* IngestionEating or drinking food or water contaminated with T2 mycotoxin* ContactAbsorption through the skinpossible symptomsInhalation: Nose/throat pain Nasal discharge Itching Sneezing Coughing up blood Chest painIngestion: Stomach pain Diarrhea Vomiting Flu-like symptoms
18OchratoxinOchratoxins are metabolites of Aspergillus ochraceus and Penicillium verrucosum in temperate regions and are present in a large variety of feeds and foods. There are four ochratoxins (A, B, C, and D) and the major mycotoxin among this group is ochratoxin A (OTA). This toxin is a contaminant of cereals, beans and other plant products. The most significant effect of ochratoxins in farm animals is nephrotoxicity. It is also immunosuppressive, teratogenic and carcinogenic.Ochratoxin is found in a large variety of foods including wheat, corn, soybeans, oats, barley, coffee beans, meats and cheese. Barley is thought to be the predominant source
19Absorption and Mechanism of action ochratoxin is absorbed from the gastrointestinal tract depending on the different species. The small intestine has been shown to be the major site of absorption and maximal absorption occurrs in the jejunum.Ochratoxins bind rapidly to serum albumin and are distributed in the blood mainly in bound form, Generally, the toxin has a long biological half life due to its high rate of binding to serum protein but there are differences between speciesIt primarily accumulates in the kidneys followed by the liver, muscle and fat. Due to this accumulation there has been a concern over the potential carryover into meat.
20Effects and signes of ochratoxin in the body 1- Hematopoietic effects (hematological disorders, blood in urine and faeces)2- Increased water consumption; kidney and bladder dysfunction3- Immunosuppression4- Gastro-intestinal effects (diarrhea : vomiting, dehydration & depression
22Hepatitis B is an infectious disease caused by the hepatitis B virus (HBV) which affects the liver It was originally known as "serum hepatitis Many people have no symptoms during the initial infection Some develop a rapid onset of sickness with vomiting ,yellow skin, dark urine and abdominal pain. Often these symptoms last a few weeks] and rarely result in death. It may take 30 to 180 days for symptoms to begin .Less than 10% of those infected develop chronic hepatitis B. In those with chronic disease cirrhosis and liver cancer may eventually develop.The virus is transmitted by exposure to infectious blood or body fluids. Infection around the time of birth is the most frequent way hepatitis B is acquired in areas of the world where the disease are the most frequent routes of infection Other risk factors include working in a healthcare setting, blood transfusions , dialysis, sharing razors or toothbrushes with an infected person, travel in countries where the infection rate is high, and living in an institution Tattooing and acupuncture led to a significant number of cases in the 1980s; however, this has become less common with improved sterility. The hepatitis B viruses cannot be spread by holding hands, sharing eating utensils or drinking glasses, kissing, hugging, coughing, sneezing, or breastfeeding. It is 50 to 100 times more infectious than HIV
23Sings and symptomsSigns and symptoms of hepatitis B, ranging from mild to severe, usually appear about one to four months after you've been infected. Signs and symptoms of hepatitis B may include:1- Abdominal pain2- Dark urine3- Fever4- Joint pain5- Loss of appetite6- Nausea and vomiting7- Weakness and fatigue8- Yellowing of your skin and the whites of your eyes (jaundice)
24mechanismHepatitis B virus primarily interferes with the functions of the liver by replicating in liver cells, known as hepatocytes . The virions bind to the host cell via the preS domain of the viral surface antigen and are subsequently internalized by endocytosis. HBV-preS-specific receptors are expressed primarily on hepatocytes; however, viral DNA and proteins have also been detected in extrahepatic sites, suggesting that cellular receptors for HBV may also exist on extrahepatic cellsDuring HBV infection, the host immune response causes both hepatocellular damage and viral clearance. Although the innate immune response does not play a significant role in these processes, the adaptive immune response, in particular virus-specific cytotoxic T lymphocytes(CTLs), contributes to most of the liver injury associated with HBV infection. CTLs eliminate HBV infection by killing infected cells and producing antiviral cytokines, which are then used to purge HBV from viable hepatocytes.] Although liver damage is initiated and mediated by the CTLs, antigen-nonspecific inflammatory cells can worsen CTL-induced immunopathology, and platelets activated at the site of infection may facilitate the accumulation of CTLs in the liver.
25transmission 1- sexual contact 2- Blood transfusions 3-re-use of contaminated needles and syringes4- vertical transmition
27prevention A- Prevention of transmission of the virus: 1-Practise safe sex2- Clear up blood or body fluids, using warm water and detergent3-Ensure surgical instruments are disposable or adequately sterilised4-Handle 'sharps' safely5-Do not permit healthcare workers (who are positive for hepatitis B e antigen (HBeAg)) to work in areas where they could be a risk to others.6-Wear goggles if there is risk of infected material splashing into the eye.B- Immunisation
28Noro virus))Norwalk virus IntroductionNoro virus is an RNA virus(taxonomic family calciviridae)that causes approximately %90 of epidemic non-bacterial outbreaks of gastroenteritis around the world.The virus:Formerly called (norwalk like virus)Highly contagiousstable in the enviromentResistant to routine disinfection methodsCarriers may not be symptomatic
29Route of transmission Fecally contaminated food and water. Person-to-person contact.Contaminated surfaces.Aerosols of the virus.
30Symptoms Symptoms usually start within 24-48 hours after exposure. VomitingWatery diarrheaAbdominal crampsHeadache,muscle achesFever(minority)DehydrationUp to %30 may be asymptomatic
31Diagnosisspecific diagnosis of the virus is routinely Polymerase chain reaction (PCR) assays or real-time PCR assays which give results in few hours.these assays are very sensitive and can detect concentrations as low as 10 particles
32preventionThe best way to prevent the spread of Norwalk viruses is by thorough handwashing after toilet use and before preparing or serving foods for at least 15 seconds. In addition, have plenty of rest and stay home until your Norwalk virus symptoms are gone.Other preventive measures are: 1. People who already have a Norwalk virus infection should never prepare food for others. 2. Those who are sick with diarrhea or vomiting should not work in daycare centers or health care facilities until these symptoms have stopped. .
333. Dispose or clean and disinfect infected materials or contaminated surfaces immediately after an incident of Norwalk virus infection in your home. 4. Eat only thoroughly cooked shellfish. Avoid food or water that might be prepared in an unsanitary way such as party foods and raw or uncooked foods. 5. Immediately wash clothing that may be contaminated with Norwalk virus after an illness incident. 6. Avoid handshaking when it's not necessary.
34References1- N. Magan and M.Olsen. (2004). Mycotoxins in food Detection and control (1st ed ). New York.2- Lucic, A., Pavlovic, M., Peraica, M., & Radic, B. (1999). Toxic effects of mycotoxins in humans, 77(7).3- Series, F. (2009). Mycotoxins in Food, (1), 1–134- Lucic, A., Pavlovic, M., Peraica, M., & Radic, B. (1999). Toxic effects of mycotoxins in humans, 77(7).5- Dienstag, J. L. (2008). Hepatitis B Virus Infection. The new England Journal of Medicine.