Presentation is loading. Please wait.

Presentation is loading. Please wait.

Food Allergies.  Most prevalent food allergy in the US.  1.5 million people suffer from the allergy.  Exposure to a small amount of peanut protein.

Similar presentations


Presentation on theme: "Food Allergies.  Most prevalent food allergy in the US.  1.5 million people suffer from the allergy.  Exposure to a small amount of peanut protein."— Presentation transcript:

1 Food Allergies

2  Most prevalent food allergy in the US.  1.5 million people suffer from the allergy.  Exposure to a small amount of peanut protein can trigger a potentially fatal reaction. How small? A standard peanut has about 200 mg of protein, and studies have shown that some people have an allergic reaction to just 2 mg—or around 1% of one peanut. Peanut Allergy

3  Peanut allergies do not cause problems with the first exposure to peanuts.  The immune system develops antibodies as a first reaction to the peanut protein.  The next time the body comes in contact with these proteins, the antibodies attack the peanut proteins.  This induces the allergic reaction which can be caused by direct contact, cross- contact and even inhalation.

4  With subsequent exposure, when the IgE antibodies on the mast cells make contact with the allergens, the mast cells release chemicals such as histamine into the bloodstream.  The histamine causes such allergic reactions as a runny nose, tearing eyes, sore throat, diarrhea, skin rashes, or difficulty breathing and swelling.  A severe reaction is termed anaphylaxis. Loss of consciousness or even death may ensue if not treated..

5  Food Allergen Labeling and Consumer Protection Act (FALCPA) 1/1/06 requires all packaged foods comply with its food allergen labeling requirements. Food Allergen Labeling and Consumer Protection Act  Eight major food allergens (peanut products as well as milk, egg, fish, crustacean shellfish, tree nuts, wheat and soybeans) must be labeled in language that is easy to understand.

6 Detecting the peanut protein in food Products exempt from plain English labeling rules: foods that are not regulated by the FDA, cosmetics and personal care products, prescription and over ‐ the ‐ counter medications or supplements, pet food, toys and crafts. *The FDA has exempted peanut oil from being labeled as an allergen.foods that are not regulated by the FDA

7 Links to the Real World HIV, Mad Cow, SARS Biodefense Cancer treatment Drug and steroid testing Pregnancy / Reproduction GMO ELISA E nzyme- L inked I mmuno s orbant A ssay

8 ELISA Antibody Structure Light chain Heavy chain Disulfide bonds

9 ELISA Antibody Structure

10 The more antigen the darker the color

11 ELISA Kit Workstation Inventory Reagents: Yellow tubesTest samples (#1-#3)2 Violet tube (+)Positive control1 Blue tube (-)Negative control1 Green tube (PA)Primary antibody1 Orange tube (SA)Secondary antibody1 Lab Equipment and Supplies: Microplate strips, pipettor, pipette tips, transfer pipette, wash buffer, paper towels, marking pen 1 2 3

12 ELISA E nzyme- L inked I mmuno s orbant A ssay

13 ELISA Kit Results

14 What Are The Reagents? And What Function Do They Perform? PBS: Phosphate buffered saline – provides stable buffered environment to maintain antibody structure Tween 20: Nonionic detergent – removes non- specifically bound proteins to reduce background and blocks protein binding sites on the polystyrene Microplates: Polystyrene – proteins absorb (bind) by hydrophobic bonds to the polystyrene

15 What Are The Reagents? And What Function Do They Perform? Antigen: Chicken gamma globulin Primary antibody: Polyclonal anti-chicken antibody made by rabbits Secondary antibody: Polyclonal anti-rabbit antibody made by goats linked (conjugated) to horseradish peroxidase (HRP) Enzyme substrate: 3,3’,5,5’ – tetramethylbenzidine (TMB) – a colorless solution that when oxidized by HRP turns blue

16

17  The reaction is an abnormal immune system response to things that are normally harmless.  When one is allergic, the immune system incorrectly identifies the substance as harmful to one's body. (ie. certain foods, medicines, plant pollen, or dust, are called allergens.)  To protect the body, on first contact, the immune system produces immunoglobulin E antibodies which become attached to mast cells. Allergic Reaction


Download ppt "Food Allergies.  Most prevalent food allergy in the US.  1.5 million people suffer from the allergy.  Exposure to a small amount of peanut protein."

Similar presentations


Ads by Google