2It consists of the following structures: The urinary system is the main excretory system, removing the majority of physiological wastes.It consists of the following structures:Kidneys x 2Ureters x 2Urinary bladderUrethraApart from the elimination of wastes, the kidneys also play a vital role in several other homeostatic functions, eg BP, blood volume, electrolyte balance, blood pH, water balance.(c)Eleshia Howell, 2012.
4The kidneys produce urine that contains metabolic waste products, including the nitrogenous compounds Urea and Uric acid, excess ions and some drugs.Urine is stored in the bladder and excreted by the process of micturition via the urethra.(c)Eleshia Howell, 2012.
5KidneysLie on the posterior abdominal wall, one on each side of the vertebral column, behind the peritoneum and below the diaphragm.The extend in length from T12 – L3, receiving some protection superiorly from the ribcage.The right kidney is usually slightly lower than the left (displaced by large Liver above).They are ‘bean’ shaped, weigh approx 150g and are embedded in a mass of fat which helps to protect them and hold them in position.(c)Eleshia Howell, 2012.
6A sheath of fibrous connective tissue, the renal fascia, encloses both the kidney and the renal fat. The adrenal glands sit atop the kidneys; affect kidney function by the secretion of hormone Aldosterone which helps to regulate the osmolarity of blood plasma.Viewed with the naked eye, there are 3 distinct areas of tissue seen in the kidney.Outer fibrous capsuleCortex – reddish-brown layerMedulla – innermost layer, pale conical shaped striations known as renal pyramids(c)Eleshia Howell, 2012.
7A hilum is present on the concave medial border, where the renal blood and lymph vessels, the ureter and nerves enter.The renal pelvis is the funnel-shaped structure that collects urine formed by the kidney. The walls of the pelvis contain smooth muscle are lined with transitional epithelial cells. Intrinsic propulsion of urine via the calyces (plural of calyx) to the renal pelvis then on to the ureter occurs without nervous system control.(c)Eleshia Howell, 2012.
9On a microscopic level, the kidney is composed of about 1-2million functional units called nephrons, and a smaller number of collecting ducts.The collecting ducts transport urine through the pyramids to the calyces and renal pelvis, giving the pyramids their striped appearance.A small amount connective tissue, containing blood vessels, nerves and lymph vessels supports the collecting ducts.(c)Eleshia Howell, 2012.
10The NephronConsists of a tubule, closed at one end; the other end opens into a collecting tubule.The closed end forms a cup-shaped structure known as the glomerular capsule or Bowman’s capsule, which almost completely encloses a network of tiny arterial capillaries ~ the Glomerulus.The rest of the nephron is about 3cm long and is described in 3 parts...(c)Eleshia Howell, 2012.
11Proximal convoluted tubule Medullary loop (Loop of Henle) Distal convoluted tubule (which leads into collecting duct).The collecting ducts unite, forming larger ducts that empty into the minor Calyx(c)Eleshia Howell, 2012.
13The kidneys receive about 20% of the cardiac output After entering the kidney at the hilum the renal artery divides into smaller arteries & arterioles. In the cortex, an afferent arteriole enters each glomerular capsule and then subdivides into a cluster of tine arterial capillaries, forming the glomerulus.Between these capillary loops are modified connective tissue cells, called mesangial cells, which are capable of a number of functions.Among the functions of mesangial cells is their ability to generate prostaglandins and mediators of inflammation (eg interleukins); sythesis of cytokines; uptake of immune complex molecules (eg. immunoglobulins, phagocytes). The smooth muscle modifications of the cells allow them to respond to vasoactive agents, modifying glomerular filtration locally.(c)Eleshia Howell, 2012.
14Location of Mesangial cells (c)Eleshia Howell, 2012.
15The blood vessel leading away from the glomerulus is the efferent arteriole. The AFFERENT vessel has a larger diameter to increase the pressure inside the glomerulus, driving filtration across the capillary walls.The efferent vessel divides into a second set of capillary network, wrapping around the renal tubules to maintain a supply of oxygen and nutrients to the local tissue, as well as removing waste products.(c)Eleshia Howell, 2012.
17The walls of the glomerulus and its capsule are a single layer of flattened epithelial cells, with the glomerular walls being more permeable than those of other capillaries.The remainder of the nephron and collecting duct are formed by single layer of simple squamous epithelium.Blood vessels of the kidneys have both sympathetic and parasympathetic nerve supply, controlling renal blood vessel diameter and blood flow (independently of autoregulation*)(c)Eleshia Howell, 2012.
18Functions of the Kidney Formation of Urine.The composition of urine reflects the exchange of substances between the nephron and blood in the renal capillaries. Waste products of protein metabolism are excreted, electrolyte levels are controlled and pH (acid-base balance) is maintained by the excretion of hydrogen ions.(c)Eleshia Howell, 2012.
19There are 3 processes involved in the formation of urine: Filtration – occurs in the semipermeable walls of the glomerulus and capsule. Some substances pass through while others remain in the capillaries. Filtration transpires due to the difference in hydrostatic pressure between the blood in glomerulus (higher) and osmostic pressure of the blood in the capsule (lower). Approx 125ml / min or 180L a day is filtered by both kidneys, but less than 1% (1- 1.5L) is excreted as urine.(c)Eleshia Howell, 2012.
21Renal blood flow is protected by a mechanism called autoregulation, whereby renal blood flow is maintained at a constant pressure across a wide range of systolic blood pressures (from mmHg). It operates independently from ANS control; therefore only inherent changes to blood pressure in the renal arteries will have an impact, eg fluctuating levels of metabolites (such as prostaglandin). In severe shock, when systolic pressure falls below 80- autoregulation fails and renal blood flow & hydrostatic pressure decreases, impairing filtration in nephrons.(c)Eleshia Howell, 2012.
22Selective Reabsorption Most reasborption from the filtrate back into the blood takes place in the proximal convoluted tubule (which is lined with microvilli). Materials essential to the body are reabsorbed here (water, electrolytes, glucose). Some reabsorption is passive and some requires active transport. Only % of filtrate reaches the loop of nephron where most of the water, sodium & chloride is absorbed, leaving approx 15-20% left to reach the distal convoluting tubule.Substances such as glucose and proteins are not usually seen in the urine as they have all been reabsorbed. Only present if there is excessive blood level of the substance. Reabsorption of nitrogenous waste is very limited, which is why there is urea, uric acid and creatinine present in urine.(c)Eleshia Howell, 2012.
23The kidney’s maximum capacity for reabsorption of a substance is known as the renal threshold. This eventuates if all the mechanisms for transport are ‘occupied’ or the tubules are overloaded.The renal threshold for substances varies according to body need at any particular time and in some cases, is regulated by hormonesParathyroid hormone – calcium, phosphateAnti-diuretic hormone – increases water reabsorptionAldosterone – increases reabsorption of water & sodium, and excretion of potassiumAtrial Natriuretic Peptide – decreases absorption of water & sodiumANP is secreted by the atria in response to stretching of the atrial wall.(c)Eleshia Howell, 2012.
24Tubular Secretion Substances not required and foreign materials, such as drugs, may not be cleared from the blood by filtration (because of the short time it remains in the glomerulus) so are cleared by secretion from the peritubular capillaries into the convoluted tubuled for excretion in the urine.(c)Eleshia Howell, 2012.
25Summary of urine formation p336Summary of urine formation(c)Eleshia Howell, 2012.
26Composition of Urine: Water 96% Urea 2% Uric acid Creatinine Ammonia SodiumPotassiumChloridesPhosphatesSulphatesOxalates2%(c)Eleshia Howell, 2012.
27Urine should be clear (not cloudy) and amber in colour A healthy adult passes 1000 – 1500ml / daySpecific gravity is normally between 1020 – ~ this will vary according to fluid intake (as will the colour & amount).Urine production is decreased during sleep and exercise.Specific gravity measures urine density, or the ability of the kidney to concentrate or dilute the urine over that of plasma.(c)Eleshia Howell, 2012.
28Kidney Function cont’d Water Balance & Urine OutputThe source of most body water is dietary food & fluid, with only a small amount of ‘metabolic water’ produced during metabolic processes.Water is excreted in urine, air, faeces and sweat.The balance between fluid intake and output is controlled by the kidneys. The minimum amount of urinary output (required to excrete body waste) is 500ml / day.(c)Eleshia Howell, 2012.
29Urinary volume in excess of the minimum is controlled mainly by ADH released from the posterior pituitary.Sensory nerve cells (osmoreceptors) in the hypothalamus, when stimulated, refer impulses to the posterior pituitary to release ADH, ie if the blood is becoming more concentrated, ADH output is increased, increasing the reabsorption of water from the distal convoluted tubules and collecting ducts. This negative feedback mechanism maintains normal blood osmotic pressure (and therefore sodium & water concentrations).(c)Eleshia Howell, 2012.
30Electrolyte BalanceAn electrolyte is any substance containing free ions that make the substance electrically conductive. The primary ions of electrolytes are sodium (Na+), potassium (K+), calcium (Ca2+), magnesium (Mg2+), chloride (Cl−), hydrogen phosphate (HPO42−), and hydrogen carbonate (HCO3−). The electric charge symbols of plus (+) and minus (−) indicate that the substance is ionic in nature and has an imbalanced distribution of electrons. Sodium is the main electrolyte found in extracellular fluid and is involved in fluid balance and blood pressure control.(c)Eleshia Howell, 2012.
31Our bodies require a subtle and complex electrolyte balance between the intracellular and extracellular environment. In particular, the maintenance of precise osmotic gradients of electrolytes is important. Such gradients affect and regulate the hydration of the body as well as blood pH, and are critical for nerve and muscle function. Various feedback mechanisms exist to keep the concentrations of different electrolytes under tight control.(c)Eleshia Howell, 2012.
32Both muscle tissue and neurons are considered electric tissues of the body. Muscles and neurons are activated by electrolyte activity between the interstitial fluid, and intracellular fluid. Electrolytes may enter or leave the cell membrane through specialized protein structures embedded in the plasma membrane called ion channels. eg, muscle contraction is dependent upon the presence of calcium (Ca2+), sodium (Na+), and potassium (K+). Without sufficient levels of these key electrolytes, muscle weakness or severe muscle contractions may occur.(c)Eleshia Howell, 2012.
33Electrolyte balance is maintained by oral, or in emergencies, intravenous (IV) intake of electrolyte-containing substances, and is regulated by hormones, generally with the kidneys flushing out excess levels. Electrolyte homeostasis is regulated by hormones such as antidiuretic hormone, aldosterone and parathyroid hormone. Serious electrolyte disturbances, such as dehydration and overhydration, may lead to cardiac and neurological complications and, unless they are rapidly resolved, will result in a medical emergency.(c)Eleshia Howell, 2012.
34Renin-angiotensin-aldosterone System: Changes in the concentration of electrolytes in the body fluids may be due to changes in:Body water contentElectrolyte levelsRenin-angiotensin-aldosterone System:Sodium is a normal constituent of urine and the amount excreted is regulated by the hormone Aldosterone (secreted by adrenal cortex).Cells in the afferent arteriole of the nephron release the enzyme Renin in response to sympathetic stimulation, low blood volume or low arterial blood pressure.(c)Eleshia Howell, 2012.
35Renin converts the plasma protein Angiotensin (produced by the liver) to angiotensin1. ACE (angiotensin converting enzyme) secreted by the lungs & proximal convoluting tubules, converts angio1 to angio2 which is a powerful vasoconstrictor and increases BP. Water and sodium absorption is increased, in turn increasing blood volume. This, then, leads to reduced renin secretion (negative feedback) and balance is restored.(c)Eleshia Howell, 2012.
36Calcium balanceRegulation of calcium levels is achieved by co- ordinated secretion of parathyroid hormone and calcitonin (thyroid).The distal collecting tubules reabsorb more calcium in response to PTH and reabsorb less in response to calcitonin secretion.(c)Eleshia Howell, 2012.
37The normal pH of urine is between 4.5 - 8. pH BalanceThe cells of the proximal convoluted tubules secrete hydrogen ions to maintain normal blood pH.In the filtrate they combine with a number of buffers, which neutralise highly acidic / alkaline substances and excrete excess ions into the urine.The normal pH of urine is betweenA diet high in animal proteins tends to produce more acidic urine (low pH) than vegetarians.(c)Eleshia Howell, 2012.
38UretersThese are the tubes that carry urine from the kidneys to the urinary bladderThey are approx 25-30cms long, 3mm diameter.Each ureter is continuous with the renal pelvis of the kidney it extends from, passing down through the abdominal cavity, behind the peritoneum, in front of the psoas muscle before passing obliquely through the posterior wall of the bladder.(c)Eleshia Howell, 2012.
40The walls of the ureters consist of 3 layers of tissue: The entry of the ureters into the posterior wall of the bladder helps to prevent backflow of urine ~ the pressure of accumulated urine in the bladder compresses the opening of the ureters, as does the muscular contraction of the bladder during micturition.The walls of the ureters consist of 3 layers of tissue:Outer fibrous layer, continuous with capsule of kidneyMiddle smooth muscle layerInner mucosal layer composed of transitional epithelium.(c)Eleshia Howell, 2012.
41Function of Ureters:Propulsion of urine from kidneys to bladder by peristaltic contraction of smooth muscle layer. This is an intrinsic property, not under autonomic control. Peristalsis originates from a ‘pacemaker’ in the minor calyx of the kidney, producing several waves a minute, sending little spurts of urine into the bladder.(c)Eleshia Howell, 2012.
42Urinary BladderThe bladder is roughly pear-shaped, but becomes more oval as it fills with urine, distending into the abdominal cavity as it fills to capacity.The bladder opens into the urethra at its lowest point, the neck.The peritoneum covers only the superior surface, but in females it surrounds the uterus posteriorly; in males it surrounds the rectum.(c)Eleshia Howell, 2012.
43The bladder wall is composed of 3 layers: Outer loose connective tissue, containing blood & lymph vessels & nervesMiddle interlacing smooth muscle & elastic tissueInner mucosal layerWhen empty, the inner lining is arranged in folds (rugae) which expand as bladder fills with urine.At ml we become aware of the need to urinate.Total capacity is approx 600ml.Internal urethral sphincter controls flow of urine from the bladder (involuntary control).(c)Eleshia Howell, 2012.
45UrethraA canal extending from the neck of the bladder to the exterior of the body (external urethral orifice – voluntary control)Longer in males than femalesIn males has dual function with reproductive system.In females, the urethra extends down behind the symphysis pubis and exits superior to the vaginal opening.(c)Eleshia Howell, 2012.
46MicturitionWhen ml of urine accumulates in the bladder, afferent autonomic nerve fibres sensitive to stretch are stimulated.In infants, this initiates the spinal reflex, causing instant urination.When nervous system is fully developed, the micturition reflex is stimulated but sensory impulses also reach the brain, creating an awareness to urinate. By learned and conscious effort, the external urethral sphincter & pelvic floor muscles can inhibit micturition.In infants, the need to urinate reaches only the spinal level. As nervous system develops, the impulses are sent to cerebral cortex and voluntary inhibition can occur.(c)Eleshia Howell, 2012.
47Involuntary loss of urine is known as incontinence. Over-distension of the bladder is painful and can be overridden by involuntary relaxation of the external urethral sphincter, allowing a small amount of urine to escape the bladder.Involuntary loss of urine is known as incontinence.(c)Eleshia Howell, 2012.
48Pathologies of the Kidneys The kidneys have considerable functional reserve meaning that impairment of renal function does not become evident until the equivalent of more than one kidney is lost. Hence the ability for a healthy person to be able to donate one of their kidneys.(c)Eleshia Howell, 2012.
49Oliguria – less than 400ml/day urinary output Terminology:Oliguria – less than 400ml/day urinary outputHaematuria – presence of blood in urineProteinuria – presence of protein in urineAnuria – absence of urineDysuria – pain on passing urineGlycosuria – presence of sugar in urineKetonuria – presence of ketones in urineNocturia – passing urine during the nightPolyuria – unusually large amounts of urineIncontinence – involuntary loss of urineFrequency of micturition – sml amounts often(c)Eleshia Howell, 2012.
50Several types, not all display inflammatory properties. GLOMERULONEPHRITIS:Several types, not all display inflammatory properties.In many cases, immune complexes damage the glomeruli (the antigen-antibody complex has formed in the kidney and lodged in walls of glomerulus, causing inflammatory response, impairing function).Extent may be diffuse or focalMay also be caused by infection, systemic conditions (eg Lupus, endocarditis), medication, disease.(c)Eleshia Howell, 2012.
51Can be the cause or the result of renal disease HYPERTENSION:Can be the cause or the result of renal diseaseClassified as Essential (benign or malignant) and Secondary. Both affect the kidneys when there is renal blood vessel damage, causing ischaemia. The reduced blood flow stimulates the renin-angiotensin-aldosterone system, which further raises blood pressure.Benign ~ gradual, progressive damage to glomeruli.Malignant ~ causes arteriosclerosis, affecting the nephrons.(c)Eleshia Howell, 2012.
52ACUTE PYELONEPHRITIS: Secondary ~ caused by long-standing kidney disease, leading to chronic renal ischaemia, worsening hypertension and renal failure.ACUTE PYELONEPHRITIS:Acute bacterial infection of renal pelvis & calyces, spreading to the kidney substance causing formation of small abscesses.May be caused by upward spread of microbes from urinary tract / bladder or by a bloode- borne infections.(c)Eleshia Howell, 2012.
53More common in males, and over 30yo age RENAL CALCULI“stones” form in the kidneys and bladder when mineral salts in the urine form solid crystals.More common in males, and over 30yo ageIf too big to pass through ureters or urethra, they can obstruct outflow, causing kidney damage and be extremely painful (renal colic). They may also cause damage to epithelial lining, causing haematuria, fibrosis & stricture (as healing occurs).(c)Eleshia Howell, 2012.
55RENAL FAILURE: Acute – sudden and severe reduction in the glomerular filtration rate and kidney function, often reversible over days / weeks if treated. Oliguria or anuria accompanied by metabolic acidosis due to the retention of hydrogen ions; electrolyte imbalance; accumulation of nitrogenous waste products; possible water retention. May occur as a result of damage to the kidneys, obstruction of outflow or reduced blood flow.(c)Eleshia Howell, 2012.
56RENAL FAILURE: Chronic – occurs when the renal reserve is lost and there is irreversible damage to approx 75% or more of nephrons. Onset is usually slow, progressing over years, with the main cause being diabetes, hypertension and glomerulonephritis. End-Stage – death is likely without renal replacement therapy (eg haemodialysis, peritoneal dialysis, kidney transplant). The excretory functions of the kidneys are lost, acid-base balance cannot be maintained and the endocrine functions of the kidneys disrupted.(c)Eleshia Howell, 2012.
57URINARY TRACT INFECTION (UTI) Infection of any part of the urinary tractUreteritisCystitisUrethritisMay spread upwards causing pyelonephritis or kidney damage.Inflammation of the epithelium causes small haemorrhages; sensory nerve endings become hyperstimulated causing frequency of urination; urine appears cloudy, sometimes ‘pink’ and has an unpleasant odour. Lower abdo pain often present.Predisposing factors – females (shorter urethra), proximity to anus, sexual intercourse may cause trauma & transfer of microbes; hormones can cause relaxation of the smooth muscle ~ possible kinking; pressure of foetus during pregnancy. Males – enlarged prostate can cause obstruction.(c)Eleshia Howell, 2012.
58URINARY INCONTINENCE Stress incontinence ~ leakage of urine occurs when intra-abdominal pressure is raised, eg coughing, laughing, sneezing, lifting. Usually caused by weakness in pelvic floor muscles & pelvic ligaments. Urge Incontinence ~ leakage follows a sudden and intense urge to void and there is inability to delay passing urine. Commonly causes by UTI, calculi, tumours. Overflow incontinence ~ overfilling of the bladder (obstruction or neurological abnormality, eg stroke, spinal cord injury, MS.(c)Eleshia Howell, 2012.