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COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE.

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Presentation on theme: "COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE."— Presentation transcript:

1 COMPARATIVE STUDY BETWEEN THE EFFECT OF HOMEOPATHIC AND ALLOPATHIC MEDICINES IN EXPERIMENTALLY INDUCED PATHOLOGY IN LABORATORY ANIMALS ETUDE COMPARATIVE DE L’ACTION DE MEDICAMENTS HOMEOPATHIQUES ET ALLOPATHIQUES SUR UNE PATHOLOGIE EXPERIMENTALE CHEZ DES ANIMAUX DE LABORATOIRE Authors: Dr. Adrian ALECU, Dr. Mariana ALECU, Dr. Romeo BREZEANU, Dr. Gabriela MARCUS, student Adriana COJOCARU University of Medicine and Pharmacy Targu-Mures ROMANIA

2 Aim To assess the effect of homeopatic remedies in laboratory animals in placebo controled studies Do homeopatic remedies act through placebo effect?

3 Material and method Mice and rats from the bio-base (experimental station) of the University of Medicine and Pharmacy Targu-Mures Various induced experimental pathology followed by treatment –homeopathic, –allopathic –combined, homeopathic and allopathic

4 Experiments The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision The antitrauma effect of the remedy Arnica in laboratory mice The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde Homeopathic treatment of grade I burns in rats with Apis and Belladonna

5 The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

6 Previous studies made around the world with homeopathic Arnica –contradictory results experimental studies with Staphysagria - missing What are the benefits in surgery? Our hypothesis – the need of administration of the remedy early after surgery

7 Material and method 75 Wistar rats, weight g 5 groups of 15 animals The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

8 Material and method Surgery technique Step 1 - tranquilization of the rats with ether The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

9 Material and method Surgery technique Step 2 - preparing the surgery zone by depilation and disinfection The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

10 Material and method Surgery technique Step 3 - performing a deep incision on the external side of the thigh, following the line of the femur until the appearance of the bone The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

11 Material and method Surgery technique Step 4 - skin suture in 3 separate points with nonresorbable silk thread The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

12 Material and method Treatment Each rat was treated through a plastic probe, starting immediately after surgery and daily administration afterwards until the complete healing of the incision The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

13 Material and method Treatment The homeopathic remedies were dissolved in water, 1 pellet in 1 ml water Group 1 - control group, sugar water as placebo, Group 2 - Arnica CH 7, Group 3 - Arnica CH 30, Group 4 - Staphysagria CH 7, Group 5 - Staphysagria CH 30. The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

14 Material and method the length of the remaining incision was measured each day the number of days necessary for the complete healing was noted for each rat The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

15 Results The rats treated homeopathically, both remedies and both dilutions, showed a shorter healing time (15-16 days) than the placebo group (21-22 days), with a statistically significant difference (p<0,001, t-test) The effect of the homeopathic remedies Arnica and Staphysagria upon the healing time of the surgical incision

16 The antitrauma effect of the remedy Arnica in laboratory mice

17 40 AKR2 white mice, 2 groups of 20 mice: control, Arnica Kocher clamp trauma at the level of the posterior limb –fractures –swelling –pain = protection of the affected limb, adynamia Assesment of the variation of the dorsoplantar diameter

18 Results Control group –Persistent swelling more than 180 minutes –adynamia –Protection of the limb Arnica CH7 group –Moderate swelling, gone in less than 105 minutes (p < 0,001) –Good general state –Ability to use the affected limb, even fractured –normal activity The antitrauma effect of the remedy Arnica in laboratory mice

19 The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

20 Pilocarpine – parasympathomimetic effect – salivation in mice Atropine (Atropa Belladonna) - parasympatholytic effect, blocking of the muscarinic receptors, imposibility for pilocarpine to produce salivation Belladonna in homeopatic dilution – does it compensate the atropinic intoxication?

21 Material and method 80 de white mice AKR2 line divided in 4 groups: 1.Assessment of the salivation produced by pilocarpine (administration of pilocarpine). 2.Assessment of the muscarinic receptors blockade by atropine, given pilocarpine (administration of atropine, then pilocarpine). 3.Assessment of supposed compensation of muscarinic blockade of atropine by homeopathic Belladonna CH7 (administration of atropine, then Belladonna CH7, then pilocarpine). 4.Control group, assessment of physiologic salivation (administration of placebo). The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

22 Material and method Step 1 – subcutaneous injection of Atropine 0,1% The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

23 Material and method Step 2 - Belladonna CH7 given by gastric probe The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

24 Material and method Step 3 – tranquilization with ethylic urethan intraperitoneally 1,5 g/kgc The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

25 Material and method Step 4 - subcutaneous injection with 1% clorhidric pilocarpine The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

26 Material and method The mice were put on filter paper The area of salivary impregnation was measured each 10 minutes for 30 minutes The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

27 Results The physiologic salivation was low, nearly immeasurable on the filter paper – group 4 The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

28 Results Pilocarpine produces an abundant salivation (known pharmacologic effect), assessed through the saliva spot area on the filter paper – group 1 The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

29 Results Atropine given before pilocarpine prevented the salivation, which was very low, nearly immeasurable on the filter paper (known pharmacologic effect) – group 2 The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

30 Results If homeopathic Belladonna CH 7 is given after atropine and before pilocarpine, the abundant salivation is present – group 3 The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

31 Results The variation of salivation in the first 30 minutes in the 4 groups The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

32 Discussions In mice the homeopathic remedy Belladonna in CH 7 dilution was able to give the atropinized organism the ability to be stimulated by pilocarpine. It is possible, in theory, that the action of the homeopathic remedy Belladonna CH 7 could be one of the following: –it acted on the muscarinic receptors, determining them to release the atropine molecules and become sensible again to pilocarpine stimulation; –it acted on the atropine molecules, determining them to release the receptors on which they were bound; –it acted on cellular level, determining possible alternative ways for the de processing of the stimulus represented by pilocarpine (activation of new receptors, alternative biochemical or neuroendocrine ways) The compensation of muscarinic blockade provoked by atropine in laboratory animals with the homeopathic remedy Belladonna

33 The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

34 Stricnina (Strychnos nux-vomica L) - adynamia, tonico- clonic convulsions, hypersensitivity to sensorial stimuli, deformation of visual and acoustic perceptions, tachycardia, tachypnea, agitation and finally death. Diazepamul, benzodiazepină - tranquilizing, muscle- relaxant, anticonvulsant Nux Vomica, Strychnos nux vomica seeds dilutions – used in cases of convulsions, aggravated by external stimuli, agitation, hypersensitivity to stimuli

35 Material and method 3 groups of 20 white mice - experimental poisoning - intraperitoneal injection of 1,5 mg/kg strychnine –Group 1 - diazepam 5 mg/kg subcutaneous 1 hour before strychnine –Group 2 - Nux Vomica CH7 orally by plastic probe immediatelly after strychnine –Group 3 - control (placebo), physiologic serum subcutaneous, sugar water by plastic probe The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

36 Material and method Strychnine poisoning: – adynamia – tachycardia – tachypnea – agitation – tonico-clonic convulsions – death The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

37 Material and method We assessed: The time until convulsionsThe time until convulsions The number of animals which had convulsionsThe number of animals which had convulsions The time until deathThe time until death The number of animals which diedThe number of animals which died The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

38 Results The time until convulsions sec Placebo group sec Placebo group sec Diazepam group sec Diazepam group sec homeopathic Nux Vomica group sec homeopathic Nux Vomica group The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

39 Results Convulsions present 100% in the Placebo and Diazepam groupConvulsions present 100% in the Placebo and Diazepam group 70% of mice had convulsions in the homeopathic Nux Vomica group70% of mice had convulsions in the homeopathic Nux Vomica group The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

40 Results Placebo group mice died in secPlacebo group mice died in sec Diazepam treated mice lived longer, secDiazepam treated mice lived longer, sec Homeopathic Nux Vomica treated mice showed the longest survival, secHomeopathic Nux Vomica treated mice showed the longest survival, sec The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

41 Results All the mice (100%) in the Placebo and Diazepam group diedAll the mice (100%) in the Placebo and Diazepam group died Homeopatically treated mice with Nux Vomica died in 80%Homeopatically treated mice with Nux Vomica died in 80% The effect of the homeopathic remedy Nux Vomica compared with Diazepam on the manifestations of strychnine poisoning in laboratory mice

42 Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

43 Formaldehyde – mastocyt reaction - histamine Apis - swelling, redness, pain, warmth Hydrocortisone Hemisuccinate (HHS) - steroidal anti-inflammatory Diclofenac - nonsteroidal anti-inflammatory

44 Material and method 70 white mice – local swelling in the posterior limb by subcutaneous formaldehyde 0.5% Dorsoplantar diameter was measured before formaldehyde and then each 15 minutes Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

45 Material and method 7 groups of 10 mice 1.Hydrocortisone Hemisuccinate 2.Diclofenac 3.Apis CH 7 4.Apis CH 15 5.Hydrocortisone Hemisuccinate and Apis CH 7 6.Hydrocortisone Hemisuccinate and Apis CH 15 7.Control group, placebo Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

46 Results Local signs of inflammation –redness –swelling –motor impairment –agitation –protection of the limb Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

47 Results Dorsoplantar diameter evolution in time (average values) Comparison of the anti-inflammatory effect of the homeopathic remedy Apis and allopathic anti-inflammatory medicines in experimental acute inflammation produced by formaldehyde

48 Homeopathic treatment of grade I burns in rats with Apis and Belladonna

49 Homeopathic treatment of grade I burns in rats with Apis and Belladonna Material and method 3 groups of 10 rats Tranquilization with ether Immersion of posterior left limb in hot water at 55°C for 20 seconds

50 Material and method Grade I burns - edema and erithema

51 Material and method Treatment by gastric probe: Group 1 - placebo as sugar water Group 2 - Apis CH 9 Group 3 - Belladonna CH 9

52 Material and method Assessment: –The number of healed rats each day –The average number of days necessary for the complete healing for each group

53 Results Apis CH 9 group showed the fastest healing, followed by the Belladonna CH 9 group The Placebo group showed the longest healing time

54 Results Apis group healed in the days 1-2, with 80% rats in the first day Belladonna group healed in the days 1-3 Placebo group healed in the days 2-4

55 Final conclusions

56 Conclusions (1/4) The homeopathic remedies Arnica and Staphysagria in the dilutions CH 7 and CH 30, given early after surgery, had a significant effect in the reduction of the healing time of the surgical incision in rats The homeopathic remedy Arnica CH 7 fights the effects of mechanic trauma in mice if given immediately after trauma In mice the homeopathic remedy Belladonna in CH 7 dilution was able to give the atropinized organism the capability to be stimulated by pilocarpine, even if the specific receptors were blocked

57 Conclusions (2/4) Homeopatic Nux Vomica CH7 was significantly more efficient than Diazepam in delaying and even preventing the convulsions in the experimental strychnine poisoning. The time until death was significantly prolonged, some of the mice treated homeopathically surviving the experiment The anti-inflammatory effect of the remedy Apis in our experiments had a higher intensity than Hydrocortisone Hemisuccinate and Diclofenac, without being antidoted by them in combinations Apis CH 9 wad more efficient than Belladonna CH 9 in the treatment of grade I burns in rats, both being more efficient than placebo

58 Conclusions (3/4) In all our experiments, the effect of homeopathic remedies was objectively assessed, reproducible and statistically significant Homeopathy is different from placebo Homeopathy acts quickly in acute Homeopathy can be associated with allopathy

59 Conclusions (4/4) Homeopathic research must be extended C'est la guerre!

60 Thank you for you attention


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