Presentation is loading. Please wait.

Presentation is loading. Please wait.

ES CELLS WERE MAINTAINED IN SERUM - FREE CULTURE WITHOUT FEEDER CELLS. ES CELLS WERE PLATED ONTO GELATIN - COATED PLATES IN N2B MEDIUM AND WERE PASSAGED.

Similar presentations


Presentation on theme: "ES CELLS WERE MAINTAINED IN SERUM - FREE CULTURE WITHOUT FEEDER CELLS. ES CELLS WERE PLATED ONTO GELATIN - COATED PLATES IN N2B MEDIUM AND WERE PASSAGED."— Presentation transcript:

1

2

3 ES CELLS WERE MAINTAINED IN SERUM - FREE CULTURE WITHOUT FEEDER CELLS. ES CELLS WERE PLATED ONTO GELATIN - COATED PLATES IN N2B MEDIUM AND WERE PASSAGED EVERY 2–4 DAYS. D ISSOCIATED CELLS WERE HARVESTED IN N2B MEDIUM, PELLETED, RESUSPENDED IN N2B AND REPLATED DIRECTLY.

4

5 FROM NEW JERSEY, USA TO SCOTLAND. TOTAL COST FOR TWO MONTHS OF WORK, TRAVEL AND REAGENTS : $10,000 TO LEARN ONE EXPERIMENT.

6 SOURCE : BAYER PROJECT TESTING 67 STUDIES PUBLISHED IN REPUTABLE SCIENCE JOURNALS.

7 P OSITION THE METAPHASE SPINDLE AT 3 O ’ CLOCK AND HOLD IT WITH HOLDING PIPETTE. A PPLY PIEZO PULSES TO PENETRATE THE ZONA PELLUCIDA. T OUCH THE METAPHASE PLATE WITH THE ENUCLEATION PIPETTE. A SPIRATE THE SPINDLE AND WITHDRAW THE PIPETTE.

8

9

10

11

12

13

14

15

16 S TANFORD U NIVERSITY N ATIONAL I NSTITUTES OF H EALTH B OSTON U NIVERSITY P RINCETON U NIVERSITY T UFTS U NIVERSITY Y ALE U NIVERSITY S ALK I NSTITUTE U NIVERSITY OF T ORONTO U NIVERSITY OF C HICAGO UCLA D ARTMOUTH C OLLEGE C ORNELL U NIVERSITY E MORY U NIVERSITY W EIZMANN I NSTITUTE N ORTHWESTERN U NIVERSITY K ING ’ S C OLLEGE L ONDON

17

18 SUBSCRIBERS : UNIVERSITIES, COLLEGES AND PHARMA / BIOTECH COMPANIES

19

20 P URDUE U NIVERSITY W EST L AFAYETTE, I NDIANA, USA E STABLISHED M AY 6, ,000 STUDENTS 8,100 POSTGRADUATES 5 S CIENCE D EPARTMENTS 100+ R ESEARCH L ABORATORIES J O VE SUBSCRIBER SINCE 2010

21 D R. T HERESA C ASEY, A SSISTANT P ROFESSOR IN THE D EPARTMENT OF A NIMAL S CIENCES AT P URDUE U NIVERSITY P H.D. FROM U NIVERSITY OF V ERMONT P RACTICING RESEARCHER FOR 20 YEARS 19 PUBLISHED PAPERS IN PEER R ESEARCH INTERESTS : MAMMARY DEVELOPMENT AND LACTATION

22 D R. C ASEY ’ S PROBLEM : “ HOW DO WE ISOLATE THE SUPRACHIASMATIC NUCLEI THROUGH SURGERY ?” D R. C ASEY DISCOVERED THAT ACTIVITY OF CIRCADIAN CLOCK GENES CHANGED DURING THE TRANSITION FROM PREGNANCY TO LACTATION IN OTHER TISSUES. C IRCADIAN CLOCK IS GOVERNED BY THE SUPRACHIASMATIC NUCLEUS ( SCN ) IN THE HYPOTHALAMUS AREA OF THE BRAIN. N EED TO LEARN TO DISSECT THE SCN BRAIN AREA USING SURGERY.

23 D R. C ASEY ’ S SOLUTION : UTILIZE A J O VE VIDEO ARTICLE AS A PRODUCTIVITY TOOL SLICE PREPARATION, ORGANOTYPIC TISSUE CULTURING AND LUCIFERASE RECORDING OF CLOCK GENE ACTIVITY IN THE SUPRACHIASMATIC NUCLEUS. SAVELYEV ET AL, 2011, J O VE (KAROLINSKA INSTITUTE)

24 T HE RESULTS : USING J O VE SAVES RESEARCHERS AND UNIVERSITIES BOTH MONEY AND TIME. F OR RESEARCHERS : D R. C ASEY SPENT TWO WEEKS TO LEARN THE EXPERIMENT. W ITHOUT J O VE IT WOULD HAVE TAKEN HER TWO MONTHS. S AVED SIX WEEKS OF WORK TIME AND $15,000 IN RESEARCH FUNDS ($7,700 REAGENTS, $1,100 TRAVEL AND $6,200 SALARY ) F OR UNIVERSITIES : I F ONLY 10% OF THE 100+ LABORATORIES AT PURDUE COULD BENEFIT FROM A SUBSCRIPTION TO J O VE, SAVINGS OF $150,000 OR MORE COULD BE REALIZED. ROI = A MINIMUM OF 500%

25 “I’ve been doing research for 20 years, and having JoVE makes things so much easier. You can educate yourself on research other scientists are doing around you and get familiarized on a technique before you try it.” - Dr. Theresa Casey

26

27

28

29


Download ppt "ES CELLS WERE MAINTAINED IN SERUM - FREE CULTURE WITHOUT FEEDER CELLS. ES CELLS WERE PLATED ONTO GELATIN - COATED PLATES IN N2B MEDIUM AND WERE PASSAGED."

Similar presentations


Ads by Google