1. Fall 2005 LAB #11: MAMMALIAN ANATOMY Histology, Fetal Pig Dissection

2. Histology The slides you will be examining Photos by T. Chubb/J. Trout

3. Epithelial Tissue

4. Kidney (Simple Cuboidal Epithelium) 40 x100x 400x

5. Kidney (Simple Cuboidal Epithelium) 40 X 400 X 100 X This is from a slightly different area of the kidney

6. Esophagus Stratified Squamous Epithelium (non-keratinized) 40 X 100 X 400 X

7. Connective Tissue

8. Bone 100 X 400 X Central canal All the little fine black lines are the caniliculi osteocyte

9. Blood http://www.gihealth.com/images/imgIronDeficiency2.jpg

10. Muscle Tissue

11. Skeletal Muscle 40 X 40 X (ls) 100 X (ls) 400 X (ls) Cross section do not use nucleus

12. Cardiac Muscle 100 x 400 x 40 x nucleus

13. Smooth Muscle 40 x 100 x 400 x (cs) 400 x (ls)

14. Nervous Tissue

15. 100x 400x Cell body of neuron

16. Fetal Pig Dissection

17. Dissection: Definition: The process of careful separation for detailed examination & analysis. TAKE YOUR TIME! Look BEFORE you cut!

18. Fetal Pig After external examination, make your initial cuts as shown (follow your lab manual). http://www.ekcsk12.org/science/lelab/incisionsfetalpig.gif

19. Internal Anatomy Following the lab manual, carefully dissect your specimen. Find all features listed on your “Checklist of Structures”. http://home.satx.rr.com/christenson/Churchill%20Biology/dissec13.jpg

20. Fetal Heart vs. Adult Heart Is there a difference? Why is there a difference? ….Think about it!

21. NORMAL HEART (Adult) NORMAL HEART www.mirage-samoyeds.com/ heart.htm

22. PATENT DUCTUS ARTERIOSIS www.mirage-samoyeds.com/ heart.htm

23. DEMO: Sheep Heart Dissection External Examination Use the demonstration sheep heart to complete your “Heart Diagram” Handout. http://science.tjc.edu/images/preserved_heart/cut_heart_anterior_label.jpg

24. DEMO: Sheep Heart Dissection, (cross section view) http:// www.sunyniagara.cc.ny.us/val/sheep-heart-3.jpg

25. DEMO: Sheep Brain (lateral view) http://www.sunyniagara.cc.ny.us/val/sheep-brain-lateral.jpg

26. DEMO: Sheep Brain (inferior view) http://www.sunyniagara.cc.ny.us/val/sheep-brain-inferior.jpg

27. TODAY’S PLAN: 1.Observe histology slides (8). Sketch & label on your “Tissue Types Handout”. (use your lab manual as a guide, Ex. 32). 2.Fetal Pig Dissection: done in pairs. Follow handout & use your manual, Ex. 33, 34*, 35. Identify all structures on the “Checklist of Structures for the Fetal Pig Dissection”. 3. Observe DEMOs: Sheep Heart, Brain, Shark.

28. Post-Lab Assignment: To be completed TODAY: (1) histology slide sketches, (2) I.D. pig anatomy, (3) adult heart diagram. To be turned in NEXT WEEK: Lab Manual Questions Exercise 34, pages 573-574.

29. CLEAN UP! Dispose of all specimens in the RED BIOHAZARD BAG/BOX provided. Wash all dissection tools & set out to dry. Wipe down your lab bench. Wash your hands before leaving.