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Tracing Neuronal Cell Development and Maturation in the Mouse Spinal Cord Kelly Probst Dr. Michael Gross Laboratory.

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Presentation on theme: "Tracing Neuronal Cell Development and Maturation in the Mouse Spinal Cord Kelly Probst Dr. Michael Gross Laboratory."— Presentation transcript:

1 Tracing Neuronal Cell Development and Maturation in the Mouse Spinal Cord Kelly Probst Dr. Michael Gross Laboratory

2 Sensory Information  Somatosensory  Visual  Auditory  Olfactory  Gustatory  Vestibular

3  Exteroceptive Touch, Temperature, Pain  Proprioceptive Body Position  Interoceptive Status of Viscera Somatosensory Information

4 Sensory Information Flow Brain Spinal Cord Body Visceral Afferents

5 AA AA Dorsal Root Ganglion (DRG)) Spinal Cord Spinal Cord Innervation by Sensory Neurons

6 Spinal Relay Interneurons Thalamus Hindbrain Cerebellum Sensory Neuron Relay Interneuron Synaptic Connection

7 Ventricular Zone Mantle Zone S M G1 G2 PMG0 Neurons Born (E10.5 to E13.5)

8 Early Embryonic Interneuron Populations VZ MZ Isl1-2 = I3 Foxd3 = I2 Lbx1 = I4

9 Project Objective Match adult ascending tracts with embryonic neural tube populations Identify when in development these relay axonal projections reach the brain ?

10 Backfill Analysis Flour-Dextran Contralateral Ipsilateral

11 Embryo Dissection Hindbrain C1 (Atlas) Cervical Vertebrae

12 Fill TMR-dextran Fill Filled Neuron

13 Incubation

14 Spinal Cord/Brain Dissection Fill Site Brachial Enlargement Cervical Region Abdominal Region Lumbar Region and Enlargement Hindbrain-Spinal Cord Junction

15 E18.5 Backfilled Spinal Cord 1 cm Section 27 (27 sections X 100  m = 2.7 mm) Dorsal Ventral

16 E17.5 Backfilled Spinal Cord 1 cm Section 10 (10 sections X 100  m = 1.0 mm) Dorsal Ventral

17 E16.5 Backfilled Lbx1 +/- Spinal Cord 1 cm Section 28 (28 sections X 40  m = 1.1 mm) Dorsal Ventral

18 Conclusions By embryonic day 16.5, the axons of some relay interneurons have reached the hindbrain A subset of these relay interneurons express Lbx1 and therefore are derived from the I4, I5, or I6 embryonic populations

19 Future Goals Backfill younger spinal cords Backfill from specific brain regions Co-label backfills with more marker combinations

20 Acknowledgements Dr. Michael Gross Dr. Kevin Ahern Howard Hughes Medical Institute


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