Presentation on theme: "Protein Synthesis. E. coli Ribosome -70S particle, MW ~2.5 x 10 6 -dissociable into small (30S) and large (50S) subunits -30S contains 16S RNA, 21 polypeptides."— Presentation transcript:
E. coli Ribosome -70S particle, MW ~2.5 x 10 6 -dissociable into small (30S) and large (50S) subunits -30S contains 16S RNA, 21 polypeptides -50S contains 5S, 23S RNA + 31 polypeptides “Although the ribosome has been crystallized…it is such a complex entity that it will be many years before its structure is known in molecular detail” - Voet and Voet, Biochemistry 1995
Puromycin “…is known to interfere with protein formation by interfering with the function of RNA in the cells involved. In goldfish studied by Bernard W. Agranoff at the University of Michigan long term memory was obliterated when the fish were given minute injections of puromycin. Since short-term memory is not much affected, it is concluded that the antibiotic interferes with the process by which memory becomes fixed in the brain” - Merck Index 1968 Tyrosyl-tRNA Puromycin Inhibition of Protein Synthesis
Protein Components of the Spliceosome -~10-220 kD -structural roles, functional roles -conserved (core), unique -non-snRNP “Comprehensive proteomic analysis of the human spliceosome.” Nature 419, 182-185 (2002).