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Chapter 15 Enzyme Specificity and Regulation
15.1 Specificity Is the Result of Molecular Recognition 15.1.1 Enzyme Specificity Structural specificity Stereospecificity
15.1.2 Hypothesis of Enzyme Action the “lock and key” hypothesis the “induced fit” hypothesis
15.1.3 Active Site of Enzyme The specific site on the enzyme where substrate binds and catalysis occurs is called the active site.
15.2 Allosteric Regulation Feedback inhibition (or regulation) A B C D Z E1 E2 E3 En A B C D Y E Z E1 E2 E3 E4En E3* E4*En*
Allosteric activator Allosteric inhibitor CO 2 + Glu + ATP CTP N-carbamoylaspartate Asp ATCase Carbamoyl phosphate Activation Inhibition
15.3 Reversible covalent modification
15.4 Proteolytic Activation
15.4.1 Pancreatic Proteases Trypsinogen Enteropeptidase Trypsin Chymotrypsinogen Proelastase Chymotrypsin Elastase
15.4.2 Blood Clotting Cascade
15.5 Regulation of Enzyme Synthesis and breakdown 15.6 Isozyme
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