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4.7 (b) To investigate the influence of carbon dioxide concentration on the rate of photosynthesis
Before you start get or make a copy of this Table of results NaHCO 3 concentration (No. of bubbles / min) Trial 1Trial 2Trial 3Average
Step 1 Fill each boiling tube with a different concentration sodium hydrogencarbonate solution and label.
Step 2 Put the boiling tubes in a water bath at 25°C until they reach this temperature.
Step 3 Cut the stem of the elodea at an angle. Remove several leaves from the cut end of the stem.
Step 4 Hook a paper clip onto the end of the elodea. Drop the elodea, stem up, into the boiling tube containing the 0.02% solution.
Step 5 Place the boiling tube containing the elodea into a water bath at 25°C.
Step 6 Switch on the light source and place the water bath containing the elodea 15 cm from the lamp. Wait for 5 minutes.
Step 7 Observe bubbles being released from the cut end of the stem. Count and record the number of bubbles released per minute. Repeat twice and get an average.
Step 8 Repeat the experiment using the different concentrations of sodium hydrogencarbonate solution.
Table of results: NaHCO 3 concentration (No. of bubbles / min) Trial 1Trial 2Trial 3Average
Expected Graph Rate of Photosynthesis Carbon dioxide concentration
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