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Comparison of Three Sources of CO 2 (Electrochemical Generation, Propane Combustion and Compressed Gas Cylinder) for Mosquito Surveillance Daniel L. Kline.

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Presentation on theme: "Comparison of Three Sources of CO 2 (Electrochemical Generation, Propane Combustion and Compressed Gas Cylinder) for Mosquito Surveillance Daniel L. Kline."— Presentation transcript:

1 Comparison of Three Sources of CO 2 (Electrochemical Generation, Propane Combustion and Compressed Gas Cylinder) for Mosquito Surveillance Daniel L. Kline USDA-ARS, CMAVE Gainesville, FL

2 CO 2 Short History 1922 Rudolfs reported that carbon dioxide was a mosquito attractant 1934 Headlee reported that delivering CO 2 gas over a New Jersey light trap for only 2 hr each evening increased the mosquito catch by % 1942 Reeves & Hammon were two of the first to seriously propose the addition of CO 2 to light traps to increase mosquito catches. Not all species are attracted equally by the addition of CO 2.

3 Available Sources Human and animal sources Dry ice Compressed (pressurized) gas cylinders Propane combustion Solid impregnate technology Yeast fermentation –Anaerobic –Aerobic

4 Available Sources Sodium bicarbonate/acid drip method Sterno combustion Fuel cells

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8 Coleman MD-2500

9 USDA CSREES grant award No , entitled “Electrochemical Carbon Dioxide Generator for Mosquito Surveillance” Dr. Henri Maget Med-e-Cell San Diego, CA

10 SBIR Phase I Overall Objective Determine the feasibility of using Med-e- Cell’s electrochemical CO 2 generation technology for mosquito surveillance.

11 Phase I Specific Objective Develop an experimental CO 2 generator capable of producing up to 200 ml of CO 2 per minute.

12 By applying voltage across an electrochemical cell containing a carboxylic acid (e.g. oxalic acid), the following reactions take place: Anode reaction: HOOC-COOH 2 CO 2 + 2H + + 2e - Cathode reaction: 2H + + 2e - H 2 Overall process: HOOC-COOH 2 CO 2 + H 2 Electrochemical Generation of CO 2

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14 Prototype Carbon Dioxide Generator The generator geometry is 6.5 x 5 x 4 cm and weighs less than 100 grams The first prototype generator operated from an AC/DC converter at 5 Amps and 3.8 volts producing 9 liters of CO 2 /hr for a period of 4 hours (150 ml/min); consumed 67 grams of oxalic acid The cost to operate the device at that rate and duration would be about 25 cents for the organic acid and 1 cent in power The size of the generator can be increased (or decreased) and the production rate of CO 2 also

15 Other Prototype Features No valves, manifolds, etc. Can be started manually (switch) or by timer The rate can be increased/decreased at will by adjusting the current The system price is in the vicinity of $100 The system is battery compatible The system is ideally suited for solar energy, since it is a low voltage device

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20 Mosquitoes Released in Each Cage Ochlerotatus taeniorhynchus (500) Aedes aegypti (500)

21 Large Cage Studies

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23 SBIR Phase II Plans Develop an integrated monitoring system which would include: –The electrochemical CO 2 generator and octenol dispenser as described in this talk –A way to generate moisture and heat as a by- product of the electrochemical process –Electronic time-release circuitry to start/stop the CO 2 and to deliver pulses of CO 2 –A solar power source to allow complete system autonomy


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