1. DArT-based linkage map of the
Solanum tuberosum ssp andigena accession LOP-868 (CIP )
Cultivar Name : Alca Tarma
Chromosomes I to XII
Maria V. Caraza Salas
Elisa J. Mihovilovich Castro
Mariela Aponte Villadoma
Merideth Bonierbale

2. Notes
Population size =174 dihaploid progenies derived from haploidization of LOP 868 with haploid inducer lines IVP-101 and of Solanum phureja Juz. et Bukasov
Total DArT markers mapped = 521
Mean DArT markers per chromosome = 43
Number of SSR markers mapped = 45
Mean SSR markers per chromosome = 4
Additionally:
A major gene denoted Rladg for resistance to PLRV identified in this accession mapped on chromosome V (Velásquez et al. 2007)
An AFLP marker P13M mapped close to the major gene for PLRV resistance
Position of QTL for late blight resistance identified by association mapping in a Solanum t. andigena-derived B1 Population on chromosomes I, II, III; and IX
Total length of the tetraploid map = cM

3. Linkage map construction
The genetic map was constructed using the statistical program TetraploidMap (Hackett and Luo 2003)
Marker order was established by the simulated annealing algorithm of TetraploidMap at starting T = 10, a cooling factor of = 0.85 and M = 100n random changes at each T°
Chromosome assignment and polarity was established by mapping SSR markers and using “sol genomics network” maps; Potato meta-QTL consensus map and Solanum phureja diploid map 2010, as reference

4. References
Hackett CA, Luo ZW (2003) TetraploidMap: construction of a linkage map in autotetraploid species. J Hered 94(4):358–359 Velásquez AC, Mihovilovich E, Bonierbale M. (2007) Genetic characterization and mapping of major gene resistance to potato leafroll virus in Solanum tuberosum ssp. andigena. Theor Appl Genet 114 (6):

5. Chromosome I
QTL for late blight resistance found in B1 population by association mapping

6. Chromosome II
QTL for late blight resistance found in B1 population by association mapping

7. Chromosome III
QTL for late blight resistance found in B1 population by association mapping

8. Chromosome IV

9. Chromosome V

10. Chromosome VI

11. Chromosome VII

12. Chromosome VIII

13. Chromosome IX
QTL for late blight resistance found in B1 population by association mapping

14. Chromosome X

15. Chromosome XI

16. Chromosome XII