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Nucleotides and DNA Structure C483 Spring 2013. 1. Purine(s) which are found mainly in both deoxyribonucleotides and ribonucleotides are A) thymine and.

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Presentation on theme: "Nucleotides and DNA Structure C483 Spring 2013. 1. Purine(s) which are found mainly in both deoxyribonucleotides and ribonucleotides are A) thymine and."— Presentation transcript:

1 Nucleotides and DNA Structure C483 Spring 2013

2 1. Purine(s) which are found mainly in both deoxyribonucleotides and ribonucleotides are A) thymine and cytosine. B) cytosine and uracil. C) cytosine. D) guanine and cytosine. E) adenine and guanine. 2. The abbreviation dGp indicates A) 5' deoxyguanylate. B) 3' deoxyguanylate. C) 3', 5' deoxyguanylate. D) 5', 3'deoxyguanylate. E) dGMP 3. Much of the stability of the double stranded helical DNA structure is the result of A) hydrogen bonding between purines. B) the phosphodiester backbone. C) Ionic nucleobase attraction. D) the stacking interactions between base pairs.

3 All five histones are rich in ________ amino acid residues whose positive charges allow binding to the sugar-phosphate backbone of DNA. A) lysine and alanine B) lysine and arginine C) leucine and alanine D) leucine and arginine Which does not apply to most bacterial DNA? A) Circular. B) Relaxed. C) Not packed into nucleosomes. D) Supercoiled. Which best describes the structure of a nucleosome core particle? A) A histone octamer with DNA threaded through its center. B) About 50 bp of DNA associated with one histone H1 molecule. C) One nucleosome plus one histone H1 and linker DNA. D) A histone octamer wrapped approximately two times around with DNA.

4 Nucleic Acid Structure Nucleobase Nucleoside Nucleotide Nucleic acid Chromatin Chromosome

5 Base Structure Purines and pyrimidines Aromatic Tautomers H-bonding

6 Nucleosides Ribonucleosides and deoxyribonucleoside Purine = osine; pyrimidine = idine (watch cytosine)

7 Nucleotides Phosphorylated on 2’, 3’, or 5’ 5’ unless noted Draw these: – dA – ADP – ppAp – ApAp pA is normally called _______ or _______________

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9 Polynucleotides Phosphate diesters polyanion directionality 5’  3’ Abbreviation is pdApdGpdTpdC tetranucleotide

10 Double Stranded DNA Chargaff’ Rule: %A =%T and %G = %C (C + G) not necessarily equal to (A + T)

11 Complementary Base Pairs Mismatching may occur with tautomers

12 Antiparallel Inaccuracy of two- dimensional drawing: bases are perpendicular to the paper “ladder” H-bonding

13 Helix Maximization of base pair stacking More compact Major and minor groove How do we explain major/minor grooves?

14 Major/Minor Groove Many pictures show ladder with backbone at 180 o Actually a distorted ladder with poles closer to each other, on one side

15 Weak Forces Stabilize Double Helix Stacking interactions (vdW forces) Hydrogen bonding Hydrophobic effect Charge-charge

16 Denaturation Melting point Melting curve UV-absorption cooperative

17 A/T Rich and G/C Rich strands GC rich strands harder to denature due to STACKING (not H-bonds) Cooperativity due to initial unstacking, which exposes bases to water, which destabilizes H- bonds, which leads to further denaturation

18 Supercoiling Bacterial DNA Closed, circular DNA Topology and topoisomerases

19 Eukaryotic DNA Chromatin 8000x packing ratio – Nucleosomes (10x) – 30nm chromatin fiber (4x) – RNA/Protein scaffold holds loops (200x condensation of DNA length

20 Nucleosomes Beads on string Histones form octamer Core particle

21 Unpacking Histones serve as negative supercoiling Histone acyltransferases (HATS) Necessary for expression

22 Chromosome Scaffold of RNA and protein 30nm fibers are looped many times Picture of histone- depleted chromosome: DNA strands have fallen off of scaffold

23 Answers 1.E 2.B 3.D 4.B 5.B 6.D


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