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The 10th International Congress of the Asian Society of Clinical Pathology and Laboratory Medicine September 10-11, 2009 in Ulaanbaatar Mongolia.

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Presentation on theme: "The 10th International Congress of the Asian Society of Clinical Pathology and Laboratory Medicine September 10-11, 2009 in Ulaanbaatar Mongolia."— Presentation transcript:

1 The 10th International Congress of the Asian Society of Clinical Pathology and Laboratory Medicine September 10-11, 2009 in Ulaanbaatar Mongolia.

2 Rapid detection and identification of causal agents is essential for timely actions to control of outbreaks and successful clinical management of the disease cases.

3 However the traditional methods of virology are time-consuming and costly, making them less successful for practicising epidemiologists and clinicians, especially to control short- duration infections like influenza.

4 In this report we share with you our results in the last 2 years with real-time Reverse- transcriptase (r-RT) PCR for this purpose.

5 In our laboratory, we have been introduced real time PCR since The study aim is to compare r-RT PCR and traditional method to satisfy epidemiological and clinical needs. We are trying to find out sensitive, rapid and cheaper methods for the reliable detection of A (H3, H1) and B influenza viruses.

6 Samples –565 clinical nasal samples from patients with ILI collected in ISSSs from Baganuur District, UB and Selenghe aimag in 2008/2009 cold season. r-RT-PCR –Instrument(ABI 7300) –RNA purification (QUIAGEN mini RNA kit) –Primer (Invitrogen®A (H1), A(H3), B, M? –Probe? ! (TaqMan) –Master-mix (Invitrogen) Traditional method –Influenza virus isolation (MDCK cell culture) –Serological tests(HA and HIT)

7 Specimen collection Virus Isolation on MDCK cell culture Subtyping: HIT ~6-7days Detection: HA

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10 Specimen collection RNA isolation r-RT-PCR 4-5 hours

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17 r-RT-PCR 6 times more sensitive 30 times quicker 2.5 times cheaper > Traditional method (virus isolation on cell culture) for influenza virus detection

18 On the basis of the obtained results r-RT_PCR is recommended for routine influenza virus surveillance.

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