Presentation on theme: "The 10th International Congress of the Asian Society of Clinical Pathology and Laboratory Medicine September 10-11, 2009 in Ulaanbaatar Mongolia."— Presentation transcript:
The 10th International Congress of the Asian Society of Clinical Pathology and Laboratory Medicine September 10-11, 2009 in Ulaanbaatar Mongolia.
Rapid detection and identification of causal agents is essential for timely actions to control of outbreaks and successful clinical management of the disease cases.
However the traditional methods of virology are time-consuming and costly, making them less successful for practicising epidemiologists and clinicians, especially to control short- duration infections like influenza.
In this report we share with you our results in the last 2 years with real-time Reverse- transcriptase (r-RT) PCR for this purpose.
In our laboratory, we have been introduced real time PCR since 2008. The study aim is to compare r-RT PCR and traditional method to satisfy epidemiological and clinical needs. We are trying to find out sensitive, rapid and cheaper methods for the reliable detection of A (H3, H1) and B influenza viruses.
Samples –565 clinical nasal samples from patients with ILI collected in ISSSs from Baganuur District, UB and Selenghe aimag in 2008/2009 cold season. r-RT-PCR –Instrument(ABI 7300) –RNA purification (QUIAGEN mini RNA kit) –Primer (Invitrogen®A (H1), A(H3), B, M? –Probe? ! (TaqMan) –Master-mix (Invitrogen) Traditional method –Influenza virus isolation (MDCK cell culture) –Serological tests(HA and HIT)
Specimen collection Virus Isolation on MDCK cell culture Subtyping: HIT ~6-7days Detection: HA