Presentation on theme: "Genetic Terms Gene - a unit of inheritance that usually is directly responsible for one trait or character. Allele - an alternate form of a gene. Usually."— Presentation transcript:
1Genetic TermsGene - a unit of inheritance that usually is directly responsible for one trait or character.Allele - an alternate form of a gene. Usually there are two alleles for every gene, sometimes as many a three or four.Homozygous - when the two alleles are the same.Heterozygous - when the two alleles are different, in such cases the dominant allele is expressed.
2Genetic TermsDominant - a term applied to the trait (allele) that is expressed irregardless of the second allele.Recessive - a term applied to a trait that is only expressed when the second allele is the same (e.g. short plants are homozygous for the recessive allele).Phenotype - the physical expression of the allelic composition for the trait under study.Genotype - the allelic composition of an organism.Punnett squares - probability diagram illustrating the possible offspring of a mating.
5Two alleles modelLet us assume a gene containing two mutually exclusive alleles:A1,A2.There are three possible combinations:A1/A1,A1/A2 and A2/A2, with initial frequencies: u,v and w.u+v+w=1Let us compute their density after one division
6First generation frequency Offsprings Mating type 2uv 0.5A1/A1+ 0.5A1/A2A1/A1xA1/A22uwA1/A2A1/A1xA2/A2U2A1/A1A1/A1xA1/A1V20.25A1/A1+0.5A1/A A2/A2A1/A2xA1/A22vw0.5A1/A2+0.5A2/A2A1/A2xA2/A2w2A2/A2A2/A2xA2/A2
7In the next generation Hardy-Weinberg Equilibrium A1/A1=u2+uw+0.25v2=(u+0.5v)2=p12A2/A1=uv+2uw+0.5v2+vw=2(u+0.5v)(w+0.5v)=2p1p2A2/A2=0.25v2+vw+w2=(w+0.5v)2=p22
8Third generation frequency Offsprings Mating type 2p13p2 0.5A1/A1+ 0.5A1/A2A1/A1xA1/A2p12p22A1/A2A1/A1xA2/A2p14A1/A1A1/A1xA1/A14p12p220.25A1/A1+0.5A1/A A2/A2A1/A2xA1/A22p1p230.5A1/A2+0.5A2/A2A1/A2xA2/A2p24A2/A2A2/A2xA2/A2
9X linked lociIf an allele is present in the X chromosome, the situation is more complicated.If qn is the allele frequency in women in the generation n, and rn the allele frequency in mens.Rn=qn-1Qn=0.5qn-1+0.5rn-12/3qn+1/3rn=2/3(0.5qn-1+0.5rn-1)+1/3qn-1=2/3qn-1+1/3rn-1=pQn-p=-0.5(qn-1-p)
11When the Hardy-Weinberg Law Fails to Apply To see what forces lead to evolutionary change, we must examine the circumstances in which the Hardy-Weinberg law may fail to apply. There are five:mutationgene migrationgenetic driftnonrandom matingnatural selection
12MutationThe frequency of gene B and its allele b will not remain in Hardy-Weinberg equilibrium if the rate of mutation of B -> b (or vice versa) changes.By itself, mutation probably plays only a minor role in evolution; the rates are simply too low. But evolution absolutely depends on mutations because this is the only way that new alleles are created. After being shuffled in various combinations with the rest of the gene pool, these provide the raw material on which natural selection can act.
13Gene MigrationMany species are made up of local populations whose members tend to breed within the group. Each local population can develop a gene pool distinct from that of other local populations.However, members of one population may breed with occasional immigrants from an adjacent population of the same species. This can introduce new genes or alter existing gene frequencies in the residents. In many plants and some animals, gene migration can occur not only between subpopulations of the same species but also between different (but still related) species. This is called hybridization. If the hybrids later breed with one of the parental types, new genes are passed into the gene pool of that parent population. This process, is called introgression.
14Genetic DriftAs we have seen, interbreeding often is limited to the members of local populations. If the population is small, hardy-Weinberg may be violated. Chance alone may eliminate certain members out of proportion to their numbers in the population. In such cases, the frequency of an allele may begin to drift toward higher or lower values. Ultimately, the allele may represent 100% of the gene pool or, just as likely, disappear from it. Drift produces evolutionary change, but there is no guarantee that the new population will be more fit than the original one. Evolution by drift is aimless, not adaptive.
16Nonrandom MatingOne of the cornerstones of the Hardy-Weinberg equilibrium is that mating in the population must be random. If individuals (usually females) are choosy in their selection of mates the gene frequencies may become altered. Darwin called this sexual selection.Assortative mating -Humans seldom mate at random preferring phenotypes like themselves (e.g., size, age, ethnicity). This is called assortative mating.
17Natural SelectionIf individuals having certain genes are better able to produce mature offspring than those without them, the frequency of those genes will increase. This is simple expressing Darwin's natural selection in terms of alterations in the gene pool. (Darwin knew nothing of genes.) Natural selection results fromdifferential mortality and/ordifferential fecundity.
18Mortality SelectionCertain genotypes are less successful than others in surviving through to the end of their reproductive period.The evolutionary impact of mortality selection can be felt anytime from the formation of a new zygote to the end (if there is one) of the organism's period of fertility. Mortality selection is simply another way of describing Darwin's criteria of fitness: survival.
19Fecundity SelectionCertain phenotypes (thus genotypes) may make a disproportionate contribution to the gene pool of the next generation by producing a disproportionate number of young. Such fecundity selection is another way of describing another criterion of fitness described by Darwin: family size. In each of these examples of natural selection certain phenotypes are better able than others to contribute their genes to the next generation. Thus, by Darwin's standards, they are more fit. The outcome is a gradual change in the gene frequencies in that population.
20Effect of Natural Selection on Gene Frequencies. Let us define the frequency of each genotype in the population as: w, and the initial allele distribution as p and q for A1 and A2.wA1/A1=1-rwA1/A2=1wA2/A2=1-s
21Fitness The average fitness is: W=(1-r)p2+2pq+(1-s)q2=1-rp2-sq2 DP=([(1-r)p2+pq]/W)-p=pq[s-(r+s)p]/W
22Hetrozygote Advantage Pn+1-(s/(r+s))=[(1-r)pn2+pnq]/W -(s/(r+s))=[(1-r)pn2+pnq]-(s/(r+s))W]/W==[(1-r)pn2+pnq]-(s/(r+s)) (1-rpn2-sqn2)]/W= (1-rpn-sqn)/W[pn-(s/(r+s)]The difference decreases to zero only for positive r and s. Thus the scenario in which both alleles can survive is Hetrozygote Advantage
23Recessive diseasesIf r>0, and s=0, the disadvantage appears only homozygotic A1.In this case: pn+1=pn(1-rpn)/(1-rpn2)1/pn+1-1/pn=1/pn[(1-rpn2)/(1-rpn)-1]=[r(1-pn2)/(1-rpn)]1/pn-1/p0=nr
24Fitness SummaryThird fix point is in the range [0,1] only if r and s have the same sign.It is stable only of both r and s are positiveIn all other cases one allele is extinct.If r>0 and s=0 then the steady state is still p=0, but is is obtained with a rate pn=1/(nr+1/p0)
25Balance between Mutation and selection. Mutations can provide a balancing force to selection.Let us assume a mutation rate of m from A2 to A1. The dynamics equation is:DP=[(1-r)p2+pq]/W-p+(1-m)*qAn equilibrium is obtained whenq=[pq+(1-s)q2]/W(1-m)1+m(1-s)/s
26SummaryIn the absence of selection an allele concentration equilibrium is obtained after one generation.In the presence of selection, usually a single allele survives.There are many mechanisms which can lead to the failure of the hardy weinberg equilibrium.