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M0120 series (α-mCD86) Binding Assessment on ELISA; M0115 series (α-hCD86) Binding Assessment on ELISA; Mono IL10 series binding assessment on ELISA In-vitro.

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Presentation on theme: "M0120 series (α-mCD86) Binding Assessment on ELISA; M0115 series (α-hCD86) Binding Assessment on ELISA; Mono IL10 series binding assessment on ELISA In-vitro."— Presentation transcript:

1 M0120 series (α-mCD86) Binding Assessment on ELISA; M0115 series (α-hCD86) Binding Assessment on ELISA; Mono IL10 series binding assessment on ELISA In-vitro efficacy evaluation of α-hCD86 end of X-2: IDO activation; In-vitro efficacy evaluation of IL10 end of X-2: pSTAT3 activation;

2 Assessment of M0120 Binding towards mCD86 by ELISA mCD86-AFH Xceptor-2 Anti- hFc-HRP mCD86-AFH Xceptor-2 Anti- hIL10-HRP

3 Assessment of M0120 X2 Binding towards IL10Ra by ELISA hIL10Ra Xceptor-2 Anti- hFc-HRP

4 Assessment of M0120 X2 Binding towards mCD86 on A20 Cell Line Xceptor-2 Anti- hFc-PE A20 CD86

5 Assessment of M0115 X2 Binding towards mCD86 by ELISA hCD86-mIg Xceptor-2 Anti- hFc-HRP

6 Assessment of M0115 X2 Binding towards IL10Ra by ELISA hIL10Ra Xceptor-2 Anti- hFc-HRP

7 pSTAT3 activation Cell line Selection – WIL2S has few IL10R expressed: – HUVEC cell line: Eur J Immunol Jan;37(1): – MC/9 cell line: Good proliferation inhibition by X2. Intracellular staining 1.08% No stain CD19 IL10R

8 IDO activation Cell line Selection: WIL2S Intracellular Staining: – Santa Cruse mAb – Epitomic poly-Ab Time course: 30 min => 24 hour


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