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Caffeine and Niacin Levels in Energy Drinks By: Liz Knisley & Kelley Smith.

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Presentation on theme: "Caffeine and Niacin Levels in Energy Drinks By: Liz Knisley & Kelley Smith."— Presentation transcript:

1 Caffeine and Niacin Levels in Energy Drinks By: Liz Knisley & Kelley Smith

2 Background Information  Caffeine toxicity results when a person takes in mg of caffeine  Symptoms: irritability, nervousness, tremors, and insomnia  Caffeine can become lethal when 1000 mg or more is consumed  Symptoms: seizures or aspiration  Detrimental effect on epilepsy control  Pregnant women consuming 6 or more cups a day can result in miscarriage or low fetal birth weight

3 Background Information  Niacin is a lipid-altering drug  Mainly known to decrease cholesterol levels  Main side effect of niacin is flushing of the skin  If 80 mg is consumed at one period of time it increases the risk of muscle damage  Supplements are usually extended release

4 Purpose and Method  Purpose : to determine whether the amount of caffeine and niacin in a specific energy drink was dangerous to the human body and in what amount.  Method : use the HPLC to test different contents of energy drinks and compare them to standards run of both caffeine and niacin

5 What We Did…  Ran standards of caffeine and niacin on HPLC  Ran diluted energy drinks to gain spectra that could be compared to the standards  Monster (16 fl. oz.)  Red Bull (8.4 fl. oz.)  5 Hour Energy (1.93 fl. oz.)  Mt. Dew Kickstart (16 fl. oz.)  Rock Star: energy x2 (16 fl. oz.)

6 HPLC  Caffeine Standards  50 ppm, 40 ppm, 30 ppm, 20 ppm, and 10 ppm  Niacin Standards  50 ppm, 40 ppm, 30 ppm, 20 ppm, and 10 ppm  Energy drinks were all diluted to 1:8

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14 Errors  Ran standards originally to compare caffeine to previous ran standards  Instrument was set to 254 nm  Niacin was not actually 250 mg like it said on bottle  Had to reweigh out the niacin for the stock solution

15 Set Backs  Originally wanted to make a 1:2 dilution of each energy drink  Spectra did not come close to the standards  Tried to dilute to 1:4 and still was off the chart  Finally a dilution of 1:8 worked  Originally wanted to use taurine but the lab did not carry it

16 Conclusion  When wanting more for your money Monster and Rockstar (energy x2) are the way to go  Mt. Dew Kickstart comes in second  Red Bull comes in third  5 hour energy is last  You would have to consume Monsters or Rockstar at a consecutive times to die from caffeine intake  Mt. Dew Kickstarts  Red Bulls  Hour Energys

17 Future Work  Test more energy drinks such as NOS that contain warning labels for levels of caffeine  Run less diluted standards of niacin so that we can compare the results  Run other compounds associated with energy drinks such as taurine

18 Citations Alpert, P. The Health Lowdown on Caffeine. Home Health Care Management Practice. [Online] 2012, 24, /156.full.pdf+html (accessed May 09, 2013). Hochholzer, W., Berg, D., Giugliano, R. The Facts Behind Niacin. Ther Adv Cardiovasc Dis. [Online] 2011, 5, (accessed May 09, 2013).


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