Presentation on theme: "Extraction of Martinella obovata bark Naomi Bryner."— Presentation transcript:
Extraction of Martinella obovata bark Naomi Bryner
Overview - Genus Bignoniaceae family 120 genera, 800 species Found in Central and South America Tropical rainforests Versatile plant Horticulture, food, crafts, timber, dyes, rituals, and medicine
Overview - Species Eye medication 13 ethnolinguistic groups 8 S. American countries Amazon Indian tribes ‘yuquilla’ From fleshy root bark Stripped, pounded, and strained Applications for curing conjunctivitis Martinelline Martinellic acid
Research goals Investigate processes for pre-extraction treatment of root bark Develop a protocol for extraction Perform FCC and identify useful fractions via TLC Isolate martinelline and martinellic acid Characterize the compounds via spectroscopic methods
Pre-extraction treatment Dr. Halligan’s provisions John Beck @ SBC Stripping bark from twigs and branches Tedious & timely 37.3 g bark material obtained Milling the plant material Blender & solvent
Extraction Merck Research Labs 1995 CH 2 Cl 2 /MeOH (1:1) Bark & solvent Sat @ rt overnight Filtered out solids Evaporated to drier conditions 6.09 g slimy residue
TLC Analysis – Hex/EtOAc (4:1) Treated with vanillin stain F1 F2 F4 F3 F6F5 Solvated fractions 111 to 160 turned from pale green to pale then dark yellow. The transition occurred at F6, making F6 visually difficult to classify as either color.
Fraction summary F1: Fractions 3-12 Blue/black on TLC plate Rf = 0.69 Oily yellow, 0.9508 g F2: Fractions 35-49 Pink on TLC plate Rf = 0.33 Pale green powder, 1.0286 g F3: Fractions 73-84 Pink/red on TLC plate Rf = 0.13 Thin yellow film, 0.0471 g F4: Fractions 111-125 Discard – same Rf as F5 & F6 Much fainter on TLC plate Solvated fraction pale in color F6: Fractions 126-130 Pink/red on TLC plate Rf = 0.10 Dark green powder, 0.2404 g F5: Fractions 131-160 Pink/red on TLC plate Rf = 0.08 Yellow/tan powder, 0.9731 g
Final thoughts Conclusions IR analysis was inconclusive NMR study was cut short F5 & F6 comparison May be multiple compounds present May apply to more fractions Future Work HPLC to complete isolation UV-vis Identify! LC-MS Complete NMR Antibacterial properties Potency Killing mechanism
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