Presentation on theme: "Human Respiratory Syncytial Virus (RSV) is the most common cause of bronchiolitis and pneumonia among infants and children, with almost everyone having."— Presentation transcript:
Restriction enzyme analysis of multivalent gene on agarose gel Lane-1: 1kb ladder Lane-2: RFM2G cut with Nco I Lane-3: pET-32 cut with Nco I and Sal I Lane-4: RFM2G cut with Nco I and Sal I Lane-5: 100kb ladder 1 2 3 4 5
CLONING PROTEIN EXPRESSION PROTEIN PURIFICATION IMMUNIZATION
E. coli BL21 cells pET-32 with FM2G Protein expression
Multivalent gene cloned into pET-32a vector Successful transformation Successful protein expression Confirmation analyses identified the created protein Immunization testing in various specimens is presently ongoing
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