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LOGO Twinkle Medicine Hunter Of Cancer LZU-China iGEM Team 2013.

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Presentation on theme: "LOGO Twinkle Medicine Hunter Of Cancer LZU-China iGEM Team 2013."— Presentation transcript:

1 LOGO Twinkle Medicine Hunter Of Cancer LZU-China iGEM Team 2013

2 LOGO Content Introduction Experimental Human Practice Results & Discussion

3 LOGO Background: About Cancer Estimated Number of Persons Alive in the U.S. Diagnosed with Cancer in 2006 (N = 11.4 M survivors) Estimated Number of Female Cancer Survivors in the U.S. in 2006 (N = 6.2 M survivors) Chap.1 Chap.2 Chap.3 Chap.4 Estimated Number of Persons Alive in the U.S. Diagnosed with Cancer on January 1, 2006 by Time From Diagnosis and Gender (Invasive/1st Primary Cases Only, N = 11.4 M survivors

4 LOGO Background: About Cancer Chap.1 Chap.2 Chap.3 Chap.4 What cause cancer? Cancer cells come from normal cells, what changed? Gene mutation

5 LOGO Chap.1 Chap.2 Chap.3 Chap.4 Background: Overview of NF-κB classical signaling pathway Activated NFkB were found in almost 70% cancer cells. NFkB signaling pathway has become a variety of malignant tumor therapy and drug discovery is an important target.

6 LOGO Chap.1 Chap.2 Chap.3 Chap.4 Background: Overview of NF-κB classical signaling pathway NF-κB Signaling Pathway FADD IκB-α NF-κB p65/50 NF-κB p65/50 IκB-α cIAP1/2 Bcl-xL/2 Survivin ICAM-1 Cyclin-D1 VEGF Gene expression Cytoplasm PKC Nucleu IKKs PMA TNF-α IL-1 TNFR IL-1R MEKKS TRADD RIP TRAFs TRAF6 TAK1 MyD88 IRAK P P P IκB-α Ub P Proteasome

7 LOGO Background: Negative feedback loop of NF-κB Chap.1 Chap.2 Chap.3 Chap.4 Negative feedback loop

8 LOGO Background: Goal How to Change the situation Chap.1 Chap.2 Chap.3 Chap.4 Traditional : LOW accuracy Mice live short/ Can’t take long-term detection ?

9 LOGO Experimental: Device Promoter: 4 copies of κB Binding sequence Chap.1 Chap.2 Chap.3 Chap.4 IκB+GFP Terminator Pref Suff pSB1C3

10 LOGO Experimental: Design Cancer Why IκBα exists? Chap.1 Chap.2 Chap.3 Chap.4 NF-κB signaling pathway feedback loop dynamis To last a long term detection Sacrifice less mice

11 LOGO Experimental: Device Promoter: 4 copies of κB Binding sequence Chap.1 Chap.2 Chap.3 Chap.4 IκB+GFP Terminator Pref Suff pSB1C3

12 LOGO Background: Goal Chap.1 Chap.2 Chap.3 Chap.4 NF-κB signal is inhibited. Oscillation represents drug’s duration time. Fluorescent light Time

13 LOGO Experimental: Results & Discussion: Results Chap.1 Chap.2 Chap.3 Chap.4 DU-145 IκBα GAPDH shRNA- IκBα - +

14 LOGO Experimental: Results & Discussion: Results Chap.1 Chap.2 Chap.3 Chap.4 DU145 Cell DU-145 IκBα GAPDH

15 LOGO Experimental: Results & Discussion: Future Goals 1. Making our drug screening system work better. 1. Improve specifity: building the fine- adjusted in other cells. 2. Shorten twinkle cycle-time: show some features in pharmacokinetics of drugs. 3. Achieve semiquantitative measure: detect the light intensity of GFP and make a standard curve. Chap.1 Chap.2 Chap.3 Chap.4

16 LOGO Experimental: Results & Discussion: Application 1. Making our drug screening system work better. 1. How to do: inject DU-145 in which have a moderate expression level of IκB. 2. Long-term drug evaluation system: tumor grow slowly, mice survive longer. 3. Sensitive system: observe twinkle instead o measuring the size of tumor. Chap.1 Chap.2 Chap.3 Chap.4

17 LOGO Experimental: Chap.1 Chap.2 Chap.3 Chap.4 Human Practice Our team had a interchange with Peking iGEM team and BIT iGEM team in August. We shared our own idea to each other, and offered advice for the problem. Our Team’s member Li Xiaomeng had been honoured to visit the BIT iGEM 2013 Team’s lab. And help them to characterize and construct a part of Cr sensor.

18 LOGO Experimental: Results & Discus Chap.1 Chap.2 Chap.3 Chap.4 Human Practice We held two promotion meetings concerning iGEM for all students in our school and Lanzhou No.1 Meddle School successfully. Meanwhile, the opening day of iGEM lab has been excellently work out with the amazing enthusiasm in this summer vacation.

19 LOGO Acknowledgements Ph.D Jinbo Yang, Huyuan Feng & Their Labs Institute of Cancer Biology and Drug Screening of Lanzhou University School of Life Science of Lanzhou University Chap.1 Chap.2 Chap.3 Chap.4

20 LOGO THANKS!


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