Presentation on theme: "New cells for new purposes: use of gingival fibroblast for arterial repair Antoine LAFONT, MD, PHD, FESC, Hopital Europeen Georges Pompidou INSERM U849."— Presentation transcript:
New cells for new purposes: use of gingival fibroblast for arterial repair Antoine LAFONT, MD, PHD, FESC, Hopital Europeen Georges Pompidou INSERM U849 PARIS-DESCARTES University FRANCE E 00-16 Inserm
AAA: which target ? Loss of smooth muscle cells Destruction of elastin network Increased activity of MMP-9 Inflammatory reaction
AAA: why cell therapy ? To restore cells To restore elastin network To control MMP-9 activity
AAA: which cell ? Gingiva: embryo-like repair –A model for arterial repair –No scar/no inflammation Gingival fibroblast: –Responsible for gingival tissue healing
HYPOTHESIS Plasticity of HGF considered as embryo like cells Autologous HGF transferred in aneurysmal wall should modify the pathological remodeling : – enlarging remodeling: aneurysm –Contractil remodeling: stenosis
METHODOLOGY Cell Culture Artery Culture Animal model –1- Healthy arteries –2- Stenosis model (Lafont A, 1995) –3- Aneurism model (Anidjar S, 1992, 1994).
HGF injection in aneurysmal arteries: Perls X 5 Perls X 10
Conclusions MMP-9: a real target to specifically treat AAA Gingival fibroblast: -able to inhibit MMP-9 expression via TIMP-1 stimulation -easy to collect/amplify Cell tansplantation: appropriate for AAA
ACKNOWLEDGEMENTS: Bernard Coulomb, CR1 Bruno Gogly, PU-PH Camille Brasselet, PHU, post-doc Eric Durand, PHU, post-doc Sylvie Séguier, MCU-PH Ludovic Couty, ingénieur de recherche Mathilde Lemitre, ITA Dominique Urbain, ITA Karima Ichegour, Agnès Bodineau, AHU, doctorante 2ème année Marco Giacci, DEA Benjamin Fournier, doctorant 2ème année Adrien Naveau, doctorant 3ème année Nicoletta Reynald, doctorante 2ème année
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