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Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems Geraldine Doran Martin Cormican Interim Salmonella Reference Laboratory Ireland.

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Presentation on theme: "Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems Geraldine Doran Martin Cormican Interim Salmonella Reference Laboratory Ireland."— Presentation transcript:

1 Molecular Typing of Salmonella enterica Serovars using Two PFGE Systems Geraldine Doran Martin Cormican Interim Salmonella Reference Laboratory Ireland

2  Serovars S. Kedougou S. Bredeney  Systems Pharmacia Gene Navigator Bio-Rad CHEF Mapper (PulseNet) Molecular Typing of S.enterica Serovars using two PFGE Systems

3  Pharmacia: 4 Subtypes  CHEF Mapper:7 Subtypes S. Kedougou Results

4 Molecular Typing of S.enterica Serovars using two PFGE Systems Comparison of 2 Systems  TimePharmacia6 Days CHEF Mapper4 Days (3)  ReproducibilityBoth reproducible  Result QualityCHEF MapperEasier to intrepret Standardised

5 PFGE of S. Kedougou using Pharmacia λ A1 A1 A2 λ A3 A3 A3 A4 λ ? A4 A4 A1 λ kb

6 PFGE of S. Kedougou using CHEF Mapper λ A1 A6 A2 λ A3 A3 A5 A4 λ A5 A5 A7 A1 λ kb

7 PFGE of S. Kedougou CHEF MapperPharmacia kb Λ A1 A1 A2 λ A3 A3 A3 A4 λ A4 A4 A4 A1 λ λ A1 A1 A2 λ A3 A3 A3 A4 λ A4 A4 A4 A1 λ

8 S Kedougou Pharmacia Reproducibility λ A1 A1 A2 λ A3 A3 A3 A4 λ ? A4 A4 A1 λ λ A1 A1 A2 λ A3 A3 A3 A4 λ A4 A4 A4 A1 λ Run 1Run kb

9 PFGE of S. Bredeney kb λ A A2 A3 λ B C D E λ F G λ A A2 A3 B C D E F G λ CHEF MapperPharmacia

10 Dendogram of S. Kedougou using Bionumerics Software

11 CHEF MapperQuicker Easier to Intrepret More Reproducible Standardised (PulseNet) Conclusions


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