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Ethanol extraction Sarsaparilla / SGR Total extract of Sarsaparilla (ST) Petroleum ether- soluble (SP) Ethyl acetate- soluble (SE) n-Butanol- soluble (SB)

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Presentation on theme: "Ethanol extraction Sarsaparilla / SGR Total extract of Sarsaparilla (ST) Petroleum ether- soluble (SP) Ethyl acetate- soluble (SE) n-Butanol- soluble (SB)"— Presentation transcript:

1 Ethanol extraction Sarsaparilla / SGR Total extract of Sarsaparilla (ST) Petroleum ether- soluble (SP) Ethyl acetate- soluble (SE) n-Butanol- soluble (SB) Water-soluble (SWT) Precipitant (SWP)Supernatant (SW) Sequential solvent extractions A B MCF-7 BGC-823 Figure S1 ** *** ** *** ** *** * * ** *** ** *** * C

2 Figure S2 PG HepG2 PC3 HT-29AGSH1299T24 B A ** *** * * * * * * * * ** *** * * ** *** ** *** ** * * * *

3 Figure S3 A PBS CTX SW ** Inhibitory Rate 47.9% H22 C Con SW 3.5  g/  l MDA-MB-231 B ** * * 48h72h MDA-MB-231 D LC3 Ⅱ /DAPI MDA-MB AGS SW %S SW %S HT-29 SW %S  g/  l

4 A D E ConH2O2H2O2 SW 1hSW 24h DCFH-DA Overlay B *** AGSHT-29 *** AGS *** HT-29 ConH2O2H2O2 SW 1hSW 24h Figure S4 AGS HT-29 AGSHT-29 * * * C ConL-NMMASW 3hSW+L-NMMA DAF-FM DA Overlay ConSW 1hSW 3hSW 24h HT-29 AGS * F

5 Figure S5 ** AGS - - 20 - - U0126(  M) SW(  g/  l) p-ERK1/2 ERK1/2  -actin AGS kDa AB


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