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PALEOSALT 2008 Bivalves - VUB Rémy Mas, Fabrice Servaes, Maité Bauwens, Céline Poulain, Philippe Claeys, Eddy Keppens, Frank Dehairs.

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Presentation on theme: "PALEOSALT 2008 Bivalves - VUB Rémy Mas, Fabrice Servaes, Maité Bauwens, Céline Poulain, Philippe Claeys, Eddy Keppens, Frank Dehairs."— Presentation transcript:

1 PALEOSALT 2008 Bivalves - VUB Rémy Mas, Fabrice Servaes, Maité Bauwens, Céline Poulain, Philippe Claeys, Eddy Keppens, Frank Dehairs

2 Objectives Understanding impact of external controls (environmental conditions) on Trace Elements and Isotopes incorporation in bivalve shells  Aquarium experiment Development of the δD salinity proxy applied to organic matter in bivalve shells  In situ work : Scheldt estuary and Auray river

3 Aquarium Experiments 1 Sampling –05 Feb 07 –Mytilus edulis –knokke Acclimation Same temperature and salinity as used for experiment Experiment –Start 19 Feb 07 –End 30 April 07 Knokke

4 T1 T2 S1 S2 S1 S2 F2 F1 F2 F1 F2 T1 S2 F2 Adult Juvenile Aquarium Experiments 2 –Temperature T1  8°C ; T2  16°C –Salinity S2  28 (sea water) δ 18 O mean = -0.31 ‰ δ 13 C DIC = -0,36 ‰ S1  18 (diluted water) δ 18 O mean = -2.85 ‰ δ 13 C DIC = -0,37 ‰ - Food : Tetracelmis suecica; δ 13 C=-30‰ F1=8mgDW/week/org. F2=16mgDW/week/org. - Age : Juvenile (<1year) Vs ‘Adult’ (~1 year old)

5 Aquarium Experiments 3 Growing (∆L of the shell) Difference statistically very significant Difference statistically significant Difference statistically not significant dL shell Student testCountAveragep Temperature T1S1F2 Vs T2S1F26101.532.230.00012 T1S2F2 Vs T2S2F28102.002.923.4E-06 Salinity T1S1F2 Vs T1S2F2681.532.000.02027 T2S1F1 Vs T2S2F110 2.242.820.00057 T2S1F2 Vs T2S2F210 2.232.922.1E-06 Food T2S1F2 Vs T2S1F110 2.232.240.94732 T2S2F2 Vs T2S2F110 2.922.820.25535 Age AT1S2F2 Vs T1S2F2781.691.460.06985 Temperature ∆LSalinity ∆L

6 Aquarium Experiments 4 C isotopic composition of mussel tissues d13C tissues Student testCountAveragep Temperature T1S1F2 Vs T2S1F21415-22.07 0.98877 T1S2F2 Vs T2S2F217 -21.92-22.080.39633 Salinity T1S1F2 Vs T1S2F21417-22.07-21.920.43207 T2S1F1 Vs T2S2F11419-21.92-21.930.90682 T2S1F2 Vs T2S2F21517-22.07-22.080.95761 Food T2S1F2 Vs T2S1F11514-22.07-21.920.19737 T2S2F2 Vs T2S2F11719-22.08-21.930.3391 Age AT1S2F2 Vs T1S2F21417-22.23-21.920.08727 Difference statistically very significant Difference statistically significant Difference statistically not significant d15N tissues Student testCountAveragep Temperature T1S1F2 Vs T2S1F214177.339.360.02165 T1S2F2 Vs T2S2F217 7.867.150.54666 Salinity T1S1F2 Vs T1S2F214177.337.860.66162 T2S1F1 Vs T2S2F114198.947.190.13884 T2S1F2 Vs T2S2F217 9.367.150.01496 Food T2S1F2 Vs T2S1F117149.368.940.45873 T2S2F2 Vs T2S2F117197.157.190.97474 Age AT1S2F2 Vs T1S2F214179.427.860.08854 N isotopic composition of mussel tissues

7 Aquarium Experiments 5 Carbonate –Sampling with microdrilling 1cm 300μm 800μm Drill line

8 Adulte s δ 18 O s = 1.20  T calc = 9.5 Juvenile δ 18 O s =1.07  T calc = 10.0 T1=8°C S2=28 δ 18 0 w = -0.31 Wanamaker et al. 2007 T(°C)=16.19-4.69*(δ 18 O s -δ 18 O w )+0.17(δ 18 O s -δ 18 O w )² Adult Juvenile

9 T2 S2 F2 δ 18 O s = -0.23  T calc = 15.8 T2 S2 F1 δ 18 O s = -0.54  T calc = 17.3 T2=16°C S2=28 δ 18 O w = -0.31

10 T2 S1 F2 δ 18 O s = -0.17  T calc = 4.8 T2=16°C S1=18 δ 18 O w = -2.85 Problem T2 S1 F1 δ 18 O s = -0.33  T calc = 5.5

11 Aquarium Experiments 6 Trace Elements (Ba, Sr, Mg, Mn…) – New machine : laser UP193FX(Fast excimer) Merchantec 193nm. coupled to a ICP-MS Thermo X series 2. (Summer 2008)

12 Scheldt & Auray 1 Sampling LOCMARIAQUER BONO 1 km 10 km KN HF GR OS Mytilus edulis Ruditapes philippinarum

13 Scheldt & Auray 2

14 Ruditapes philippinarum Shell organic matrix Mytilus edulis Shell organic matrix Scheldt & Auray 3 Sea δ 13 C & δ 15 N from bivalves –Tissues –Shell OM

15 Scheldt & Auray 4 Lipids –GC-MS Extraction of total lipids (modified Bligh & Dyer extraction) from bulk shell (without decarbonatation) Derivatization : Silylation BSTFA 60°C, 30min Method GC : –Column : DB-5 30m, Ø 0.25mm –50°C (2min)300°C (15min) 4°C/min

16 Mytilus edulis Ruditapes

17 Fatty acids Sterols Me 038 KN 17:0 18:0 19:0 20:0 21:0 15:0 19:1 Mytilus edulis

18 Scheldt & Auray 5 In the near future : GC-MS –Precise identification –Quantitative measurement GC-IRMS –δ 13 C measurements –Comparison of δ 13 C of specific compounds with estuarine gradient

19 Perspectives Improvement of Mollusk shell Lipids extraction : Collaboration project with B. Farre; University Paris Sud; BIOCALC project; EUROCORES Program Deuterium analyses on specific lipidic compounds in collaboration with Dr. Stefan Schouten, Royal NIOZ

20 Thank you

21

22 R TimeIntensityFormulaProb. 5.1542855000C8H18F3NOSi270.9Acetamide, 2,2,2-trifluoro-N,N-bis(trimethylsilyl)- 9.1758533636C9H22OSi29.7Methoxydi(tert-butyl)silane 16.2863595912C6H14O2Si27.2Silanol, trimethyl-, propanoate 17.56588324608C10H26O3Si275.703,6,9-Trioxa-2,10-disilaundecane, 2,2,10,10-tetramethyl- 18.8333368116C12H32O3Si387.5Trimethylsilyl ether of glycerol 22.5831632980C10H22N2O2Si270.92,4(1H,3H)-Pyrimidinedione, dihydro-1,3-bis(trimethylsilyl)- 25.85101882128C21H24O212Benzene, 1,1'-(1-methylethylidene)bis[4-(2-propenyloxy)- 28.4347217040C13H20OSi17.94-Trimethylsilyloxy-4-phenylbut-1-ene 35.0686691232C17H36O2Si93.5Tetradecanoic acid, trimethylsilyl ester 37.4957155768C18H38O2Si68.5n-Pentadecanoic acid, trimethylsilyl ester 39.19138714112C19H38O2Si80Palmitelaidic acid, trimethylsilyl ester 39.86434806720C19H40O2Si94.2Hexadecanoic acid, trimethylsilyl ester 43.59113528232C21H42O2Si31trans-9-Octadecenoic acid, trimethylsilyl ester 44.22276898496C21H44O2Si94.7Octadecanoic acid, trimethylsilyl ester 46.4926053202C23H50OSi30.53,7,11,15-Tetramethyl-hexadecanol, trimethylsilyl ether 54.3240401216C27H56O4Si264.21-Monooleoylglycerol trimethylsilyl ether 55.4816958486C27H56O2Si56.6Tetracosanoic acid, trimethylsilyl ester 60.5245148768C30H54OSi61.2Cholesterol trimethylsilyl ether 64.6951135540C31H50P253.6Methylenebis(2,4,6-triisopropylphenylphosphine)

23 17.56 25.85 37.49 39.19 35.06 28.43 22.58 18.83

24 39.86 43.59 44.22 46.49 54.32 55.48 60.52 64.69

25 Aquarium Experiments Tissue Dry Weight after the end of the experiment Soft Tissue Dry Weight Student testCountAveragep Temperatur e T1S1F2 Vs T2S1F25824.4223.380.69138 T1S2F2 Vs T2S2F28923.0324.570.6243 Salinity T1S1F2 Vs T1S2F25824.4223.030.68471 T2S1F1 Vs T2S2F17920.0026.48 0.0892 8 T2S1F2 Vs T2S2F28923.3824.57 0.6598 6 Food T2S1F2 Vs T2S1F18723.3820.000.24168 T2S2F2 Vs T2S2F19924.5726.480.56811 Age AT1S2F2 Vs T1S2F26845.7823.030.0001 Difference statistically very significant Difference statistically significant Difference statistically not significant Difference before experiment


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