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PALEOSALT 2008 Bivalves - VUB Rémy Mas, Fabrice Servaes, Maité Bauwens, Céline Poulain, Philippe Claeys, Eddy Keppens, Frank Dehairs.

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Presentation on theme: "PALEOSALT 2008 Bivalves - VUB Rémy Mas, Fabrice Servaes, Maité Bauwens, Céline Poulain, Philippe Claeys, Eddy Keppens, Frank Dehairs."— Presentation transcript:

1 PALEOSALT 2008 Bivalves - VUB Rémy Mas, Fabrice Servaes, Maité Bauwens, Céline Poulain, Philippe Claeys, Eddy Keppens, Frank Dehairs

2 Objectives Understanding impact of external controls (environmental conditions) on Trace Elements and Isotopes incorporation in bivalve shells  Aquarium experiment Development of the δD salinity proxy applied to organic matter in bivalve shells  In situ work : Scheldt estuary and Auray river

3 Aquarium Experiments 1 Sampling –05 Feb 07 –Mytilus edulis –knokke Acclimation Same temperature and salinity as used for experiment Experiment –Start 19 Feb 07 –End 30 April 07 Knokke

4 T1 T2 S1 S2 S1 S2 F2 F1 F2 F1 F2 T1 S2 F2 Adult Juvenile Aquarium Experiments 2 –Temperature T1  8°C ; T2  16°C –Salinity S2  28 (sea water) δ 18 O mean = ‰ δ 13 C DIC = -0,36 ‰ S1  18 (diluted water) δ 18 O mean = ‰ δ 13 C DIC = -0,37 ‰ - Food : Tetracelmis suecica; δ 13 C=-30‰ F1=8mgDW/week/org. F2=16mgDW/week/org. - Age : Juvenile (<1year) Vs ‘Adult’ (~1 year old)

5 Aquarium Experiments 3 Growing (∆L of the shell) Difference statistically very significant Difference statistically significant Difference statistically not significant dL shell Student testCountAveragep Temperature T1S1F2 Vs T2S1F T1S2F2 Vs T2S2F E-06 Salinity T1S1F2 Vs T1S2F T2S1F1 Vs T2S2F T2S1F2 Vs T2S2F E-06 Food T2S1F2 Vs T2S1F T2S2F2 Vs T2S2F Age AT1S2F2 Vs T1S2F Temperature ∆LSalinity ∆L

6 Aquarium Experiments 4 C isotopic composition of mussel tissues d13C tissues Student testCountAveragep Temperature T1S1F2 Vs T2S1F T1S2F2 Vs T2S2F Salinity T1S1F2 Vs T1S2F T2S1F1 Vs T2S2F T2S1F2 Vs T2S2F Food T2S1F2 Vs T2S1F T2S2F2 Vs T2S2F Age AT1S2F2 Vs T1S2F Difference statistically very significant Difference statistically significant Difference statistically not significant d15N tissues Student testCountAveragep Temperature T1S1F2 Vs T2S1F T1S2F2 Vs T2S2F Salinity T1S1F2 Vs T1S2F T2S1F1 Vs T2S2F T2S1F2 Vs T2S2F Food T2S1F2 Vs T2S1F T2S2F2 Vs T2S2F Age AT1S2F2 Vs T1S2F N isotopic composition of mussel tissues

7 Aquarium Experiments 5 Carbonate –Sampling with microdrilling 1cm 300μm 800μm Drill line

8 Adulte s δ 18 O s = 1.20  T calc = 9.5 Juvenile δ 18 O s =1.07  T calc = 10.0 T1=8°C S2=28 δ 18 0 w = Wanamaker et al T(°C)= *(δ 18 O s -δ 18 O w )+0.17(δ 18 O s -δ 18 O w )² Adult Juvenile

9 T2 S2 F2 δ 18 O s =  T calc = 15.8 T2 S2 F1 δ 18 O s =  T calc = 17.3 T2=16°C S2=28 δ 18 O w = -0.31

10 T2 S1 F2 δ 18 O s =  T calc = 4.8 T2=16°C S1=18 δ 18 O w = Problem T2 S1 F1 δ 18 O s =  T calc = 5.5

11 Aquarium Experiments 6 Trace Elements (Ba, Sr, Mg, Mn…) – New machine : laser UP193FX(Fast excimer) Merchantec 193nm. coupled to a ICP-MS Thermo X series 2. (Summer 2008)

12 Scheldt & Auray 1 Sampling LOCMARIAQUER BONO 1 km 10 km KN HF GR OS Mytilus edulis Ruditapes philippinarum

13 Scheldt & Auray 2

14 Ruditapes philippinarum Shell organic matrix Mytilus edulis Shell organic matrix Scheldt & Auray 3 Sea δ 13 C & δ 15 N from bivalves –Tissues –Shell OM

15 Scheldt & Auray 4 Lipids –GC-MS Extraction of total lipids (modified Bligh & Dyer extraction) from bulk shell (without decarbonatation) Derivatization : Silylation BSTFA 60°C, 30min Method GC : –Column : DB-5 30m, Ø 0.25mm –50°C (2min)300°C (15min) 4°C/min

16 Mytilus edulis Ruditapes

17 Fatty acids Sterols Me 038 KN 17:0 18:0 19:0 20:0 21:0 15:0 19:1 Mytilus edulis

18 Scheldt & Auray 5 In the near future : GC-MS –Precise identification –Quantitative measurement GC-IRMS –δ 13 C measurements –Comparison of δ 13 C of specific compounds with estuarine gradient

19 Perspectives Improvement of Mollusk shell Lipids extraction : Collaboration project with B. Farre; University Paris Sud; BIOCALC project; EUROCORES Program Deuterium analyses on specific lipidic compounds in collaboration with Dr. Stefan Schouten, Royal NIOZ

20 Thank you

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22 R TimeIntensityFormulaProb C8H18F3NOSi270.9Acetamide, 2,2,2-trifluoro-N,N-bis(trimethylsilyl) C9H22OSi29.7Methoxydi(tert-butyl)silane C6H14O2Si27.2Silanol, trimethyl-, propanoate C10H26O3Si ,6,9-Trioxa-2,10-disilaundecane, 2,2,10,10-tetramethyl C12H32O3Si387.5Trimethylsilyl ether of glycerol C10H22N2O2Si270.92,4(1H,3H)-Pyrimidinedione, dihydro-1,3-bis(trimethylsilyl) C21H24O212Benzene, 1,1'-(1-methylethylidene)bis[4-(2-propenyloxy) C13H20OSi17.94-Trimethylsilyloxy-4-phenylbut-1-ene C17H36O2Si93.5Tetradecanoic acid, trimethylsilyl ester C18H38O2Si68.5n-Pentadecanoic acid, trimethylsilyl ester C19H38O2Si80Palmitelaidic acid, trimethylsilyl ester C19H40O2Si94.2Hexadecanoic acid, trimethylsilyl ester C21H42O2Si31trans-9-Octadecenoic acid, trimethylsilyl ester C21H44O2Si94.7Octadecanoic acid, trimethylsilyl ester C23H50OSi30.53,7,11,15-Tetramethyl-hexadecanol, trimethylsilyl ether C27H56O4Si Monooleoylglycerol trimethylsilyl ether C27H56O2Si56.6Tetracosanoic acid, trimethylsilyl ester C30H54OSi61.2Cholesterol trimethylsilyl ether C31H50P253.6Methylenebis(2,4,6-triisopropylphenylphosphine)

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25 Aquarium Experiments Tissue Dry Weight after the end of the experiment Soft Tissue Dry Weight Student testCountAveragep Temperatur e T1S1F2 Vs T2S1F T1S2F2 Vs T2S2F Salinity T1S1F2 Vs T1S2F T2S1F1 Vs T2S2F T2S1F2 Vs T2S2F Food T2S1F2 Vs T2S1F T2S2F2 Vs T2S2F Age AT1S2F2 Vs T1S2F Difference statistically very significant Difference statistically significant Difference statistically not significant Difference before experiment


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