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CAPTEX T2. EFFECT OF THE DIFFERENT MYCOTOXINS IN ON THE DIFFERENT FARMED ANIMALS SPECIES Poultry 1.Broilers 2.Layers 3.Breeder Sheep Goats Fish Shrimp.

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Presentation on theme: "CAPTEX T2. EFFECT OF THE DIFFERENT MYCOTOXINS IN ON THE DIFFERENT FARMED ANIMALS SPECIES Poultry 1.Broilers 2.Layers 3.Breeder Sheep Goats Fish Shrimp."— Presentation transcript:

1 CAPTEX T2

2 EFFECT OF THE DIFFERENT MYCOTOXINS IN ON THE DIFFERENT FARMED ANIMALS SPECIES Poultry 1.Broilers 2.Layers 3.Breeder Sheep Goats Fish Shrimp Mycotoxins Aflatoxin (AFB) Fumonisin (FB) Ochratoxin (OTA) T-2 Toxin (T-2) Zearalenone (ZEN) Vomitoxin or Deoxynivalenol (DON)

3 MYCOTOXIN CONCENTRATIONS IN COMPLETE FEED IN EUROPE FEEDAFFUMONISINOTAT2DONZEA % positive samples Av. Concentration (ppb) (318 samples from 10 countries, 2012)

4 3 different strategies to counteract a broad spectrum of mycotoxins 1.Preventing further mold growth 2.Adsorption by minerals 3.Biotransformation by enzymatic activity HOW DOES CAPTEX T2 DEACTIVATES MYCOTOXINS (MODE OF ACTION)

5 Sodium Propionate  acts as a mold inhibitor to prevent mold growth and proliferation  minimizes risk of having mycotoxin-producing molds further proliferating in feed.  has no activity against mycotoxins, but, always, when you ascertain mycotoxins presence, there is still a residual presence of molds. 1. PREVENTING FURTHER MOLD GROWTH

6 Minimum inhibitory concentration of propionic acid (g/kg diet) on bacteria and molds 1. PREVENTING FURTHER MOLD GROWTH

7  Through a number of screening studies the best aluminosilicate adsorbents with regard to mycotoxin deactivation and safe application where selected.  A premium blend of minerals was designed that as part of CAPTEX T2 product guarantees maximum, pH-independent activity at an inclusion rate as low as 1 kg/ton without removing essential nutrients from the diet. 2. ABSORPTION BY MINERALS

8 CAPTEX T2 contains 2 main aluminosilicates 1.Calcium Bentonite (montmorillonite) 2.Zeolite (clinoptilolite) 2. ADSORPTION BY MINERALS

9  originally created from the breakdown (weathering) of volcanic ash.  is a silicate with a layered polar crystalline microstructure which adsorbs organic substances either on its external surfaces or within its interlaminar spaces.  Used to bind mainly Aflatoxin. BENTONITE / MONTMORILLONITE 2. ABSORPTION BY MINERALS

10 MOLECULAR STRUCTURES OF BENTONITE (MONTMORILLONITE) 2:1 clay structure: one octahedral sheet sandwiched between two tetrahedral sheets The mycotoxins that are bound by the montmorillonite are those that can physically enter into the interlayer space. The width of the interlayer space is 0.25 to 0.7 nanometers in the dry state and 1 nanometer in the hydrated state. Aflatoxins and ochratoxins can enter into this space 2. ABSORPTION BY MINERALS

11  is of (sea or lake) sedimentation origin with a unique, complex crystalline structure.  honeycomb (tetrahedral) framework of cavities and channels act like cages, trapping mycotoxins.  Used to deactivate Zearalenone, Ochratoxin and Fumonisin. ZEOLITES / CLINOPTILOLITE 2. ABSORPTION BY MINERALS

12 MOLECULAR STRUCTURES OF CLINOPTILOLITE 3-dimentional crystalline Structure with an 8-ring and 10-ring channels has a cage-like structure, with pores and channels running through the crystal. The cage and surrounding mineral carries a net negative charge, making it one of the few negatively charged minerals found in nature 2. ABSORPTION BY MINERALS

13 MOLECULAR STRUCTURES OF BENTONITE (CLINOPTILOLITE) Because of its cage-like structure and negative charge, clinoptilolite has the ability to draw and trap within and on itself positively charged toxic particles that fit into the pores and channels of the cage It acts as molecular sieves and absorb substances of a low-molecular compounds (mycotoxins) Used to adsorb Zearalenone, Ochratoxin and Fumonisin, Deoxynivalenol 2. ABSORPTION BY MINERALS

14  Belongs to a new generation of mycotoxin deactivators, which are classified as highly purified and activated clays.  The minerals undergo a special process that involves;  Physical treatment (Micronisation)  Chemical treatment which greatly increases its adsorbent efficiency CAPTEX 2. ABSORPTION BY MINERALS

15  The high affinity of binding is due to the fact that the product is modified by the micronisation process to be extremely fine, 150,000 particles/gram.  This provides for a larger surface area that increases the possibility of interacting with mycotoxins.  Though extremely fine, caution is given not to have any particles size of less than 5 microns in order to avoid dustiness as well as inhalation issues with the people that are handling the product. PHYSICAL TREATMENT AND ACTIVE SURFACE OF CAPTEX T2 2. ABSORPTION BY MINERALS

16  CEC (Cationic Exchange Capacity) is important as it explains the water absorption capacity of the product.  The lower the CEC the better it is, as its water absorption capacity decreases. In the other hand, if CEC is below of 35 then it starts losing its affinity to mycotoxins.  CEC over 100, means that the product has high in water absorption capacity and consequently some nutrients can be trapped.  CAPTEX T2 has a CEC of approximately 55. IDEAL!! CHEMICAL TREATMENT AND CEC OF CAPTEX T2 2. ABSORPTION BY MINERALS

17 CAPTEX T2 DOES NOT BIND EITHER NUTRIENT OR DRUGS! MAXIMUM SIZE OF CAPTEX T2 HOLES IS 50 MICRONS Name of compoundMinimum particle size (microns) VITAMIN A350 VITAMIN D3200 VITAMIN B12120 FOLIC ACID120 IRON SULFATE250 ZINC SULFATE160 TYLOSINE150 AMOXYCILLIN150 Name of mycotoxins Maximum particle size (microns) AFLATOXIN1 ZEARALENONE1 OCHRATOXIN1 FUMONISIN1 T21 VOMITOXIN1 2. ABSORPTION BY MINERALS

18 These treatments enable the minerals to form a stable irreversible complex that immobilizes the target mycotoxins in the gastrointestinal tract of animals and by reducing their bio- availability, they are prevented from being absorbed through the gut and into the blood circulation, so thus eliminated through faeces. 2. ABSORPTION BY MINERALS PHYSICAL & CHEMICAL TREATMENT

19  is the enzymatic degradation of mycotoxins that leads to non- toxic metabolites. In this case, Chitinase is this hydrolytic enzyme capable of deactivating mycotoxins by degrading their molecules.  Chitinase is incorporated into yeast cells by our patented process and becomes active only at intestinal level when yeast cells are lyzed by the intestinal enzymes and cell content is released.  Chitinase is able to form non toxic de-epoxy metabolite by removing oxygen from the epoxide group of the trichothecene mycotoxin.  This action mimics the detoxifying process carried out by carboxylesterase (a microsomal enzyme from liver) that selectively hydrolyses the C-4 acetyl group of T-2 toxin. 3. BIOTRANSFORMATION

20 Molecule of T-2 and what happens to it after Biotransformation by Chitinase 3. BIOTRANSFORMATION

21 BIOTRANSFORMATION OF T-2 TOXIN This action mimics the detoxifying process carried out by carboxylesterase (a microsomal enzyme from liver) that selectively hydrolyses the C-4 acetyl group of T-2 toxin to yield HT-2 toxin 3. BIOTRANSFORMATION

22  Prior to their inclusion into CAPTEX-T2, Doxal’s Esterified Glucomannans are treated by another Chitinase, an enzyme which is able to reduce, dramatically, their chitin content.  Chitin content is supposed to increase the alkali insolubility of β-glucans and to decrease the cell wall flexibility, so that the toxin molecule has a restricted access to the complexing chemical sites. Not all glucomannans are the same! CAPTEX T2

23 Yeast Sources Total Glucans (%)  (1.3)- glucans (%)  (1.3)- glucans (%) Chitin (%) Saccharomyces cerevisiae Saccharomyces cerevisiae Saccharomyces cerevisiae Saccharomyces cerevisiae Doxal’s S.C Vopato I. Bizzini B Italian Project M.S.T./09/96 GLUCANS AND CHITIN CONTENTS OF VARIOUS SOURCES OF SACCHAROMYCES CEREVISIAE

24 Toxins binding capacity of three feed additives in vitro Each mycotoxin was solved at the level of 50 µg into 200 ml of methanol, and kept under gentle stirring throughout the test period, in a 250 ml volumetric flask. 100 mcg and 250 mcg aliquots of each Clay, Bentonite and CAPTEX- T2 were solved into the flasks, and kept under gentle stirring for 20 minutes; one flask of each mycotoxins was left as blank. After 20 minutes, small aliquots were collected from each volumetric flask and then assayed by High Performance Liquid Chromathography. CAPTEX T2 TRIALS

25 AFLATOXIN CLAY AT 100 MCG7.40MCG CLAY AT 250 MCG2.40MCG BENTONITE AT 100 MCG7.40MCG BENTONITE AT 250 MCG1.00MCG CAPTEX-T2 AT 100 MCG4.05MCG CAPTEX-T2 AT 250 MCG0.00MCG BLANK49.90MCG Toxins binding capacity of three feed additives in vitro CAPTEX T2 TRIALS

26 ZEARALENONE CLAY AT 100 MCG42.60MCG CLAY AT 250 MCG36.60MCG BENTONITE AT 100 MCG27.70MCG BENTONITE AT 250 MCG20.00MCG CAPTEX-T2 AT 100 MCG15.10MCG CAPTEX-T2 AT 250 MCG9.90MCG BLANK49.70MCG Toxins binding capacity of three feed additives in vitro CAPTEX T2 TRIALS

27 OCHRATOXIN CLAY AT 100 MCG6.90MCG CLAY AT 250 MCG3.10MCG BENTONITE AT 100 MCG7.00MCG BENTONITE AT 250 MCG2.95MCG CAPTEX-T2 AT 100 MCG3.60MCG CAPTEX-T2 AT 250 MCG0.20MCG BLANK50.10MCG Toxins binding capacity of three feed additives in vitro CAPTEX T2 TRIALS

28 FUMONISIN CLAY AT 100 MCG47.48MCG CLAY AT 250 MCG46.10MCG BENTONITE AT 100 MCG44.90MCG BENTONITE AT 250 MCG42.50MCG CAPTEX-T2 AT 100 MCG27.40MCG CAPTEX-T2 AT 250 MCG15.05MCG BLANK49.30MCG Toxins binding capacity of three feed additives in vitro CAPTEX T2 TRIALS

29 T-2 TOXIN Activated carbon AT 100 MCG MCG Activated carbon AT 250 MCG MCG CAPTEX-T2 AT 100 MCG MCG CAPTEX-T2 AT 250 MCG MCG BLANK MCG TOXINS BINDING CAPACITY OF CAPTEX ON T-2 TOXIN IN VITRO CAPTEX T2 TRIALS

30 TRIAL IN VIVO POULTRY FIELD - RESULTS Feed consumption Body weight mortality Plasmatic calcium g/dayg%mM/L group A 24,73441,662,180 ± 0,16 group B 16,4265,48,331,64 ± 0,66 group C 23,1326,41,662,09 ± 0,22 CAPTEX T2 TRIALS

31 THANKS FOR THE KIND ATTENTION


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