Presentation on theme: "CAVIMC 040 mAb Linear Epitope Mapping Shaunna Shen/Georgia Tomaras, CAVIMC 24 mAb + 2 negative control tested: 20 µg/ml (default), 5, 10, or 40 µg/ml (for."— Presentation transcript:
CAVIMC 040 mAb Linear Epitope Mapping Shaunna Shen/Georgia Tomaras, CAVIMC 24 mAb + 2 negative control tested: 20 µg/ml (default), 5, 10, or 40 µg/ml (for repeats/confirmation). Peptide sequences on slide: 15-mers overlapping by 12, covering full length consensus Env gp160 of HIV clade A, B, C, D, group M, and CRF 1 & 2. Slide Lots used: JPT 1948 (default) and JPT 2049 (for repeats/confirmation).
X-axis: Peptide#, ordered from N’ to C’ on gp160. Y-axis: Signal intensity, as determined by image analysis of the chip scans. Colors of bars represent different clades/group/CRFs of HIV Env. Background subtraction: 5-fold the signal intensity for nonspecific peptide binding. Results are summarized in slide 3 as a table. Graphs of data for each mAb follow (Note: In the graphs, the y-axis scales differ based on response magnitude). Binding intensity values per clade for each mAb with positivity calls are shown in a table after the graph. CAVIMC 040 mAb Linear Epitope Mapping
N10-U1 Signal Intensity Values Peptide #Clade AClade BClade CClade DGroup MCRF 1CRF µg/ml, Lot # JPT2049, PMT540; SXS Note: “Breakage” of the gp41 ID epitope at peptide #192 and 193 is a known slide quality issue. The epitope is very likely a continuous one. Background subtraction: 5-fold nonspecific peptide binding.