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Symposium on Light in Horticulture, Tsukuba, Japan Development of LED Lids for Tissue Culture Lighting Wei FANG*, National Taiwan Univ. Chi-Chung CHEN,

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Presentation on theme: "Symposium on Light in Horticulture, Tsukuba, Japan Development of LED Lids for Tissue Culture Lighting Wei FANG*, National Taiwan Univ. Chi-Chung CHEN,"— Presentation transcript:

1 Symposium on Light in Horticulture, Tsukuba, Japan Development of LED Lids for Tissue Culture Lighting Wei FANG*, National Taiwan Univ. Chi-Chung CHEN, Nano Bio light Technology Co. Yung-Yi LEE, National Museum of Natural Science Ming-Yih CHANG, National Ilan University Symposium on Light in Horticulture, Tsukuba, Japan Nov. 15-19, 2009

2 Symposium on Light in Horticulture, Tsukuba, Japan LEDLED L ight up the E co-friendly D ream in Horticulture

3 Symposium on Light in Horticulture, Tsukuba, Japan 1.Distance from lamp to plant is long. 2.Uniformity on cultural bed is poor. 3.Space utilization is low (maybe not a problem for research oriented lab.)

4 Symposium on Light in Horticulture, Tsukuba, Japan 4.Usable life span of TFL lamp is short. 7.Relative quantum yield is low, due to too much green light given by TFL. 6.Cost to remove extra heat is high. 5.Ability to keep consistent output is poor.

5 Symposium on Light in Horticulture, Tsukuba, Japan Relative quantum yield

6 Symposium on Light in Horticulture, Tsukuba, Japan Purposes of the study 1. To develop a TC vessel with LED light source attached. 2. To develop a TC cart for photo-phyto related research using light source developed. 3. To develop a TC system for mass production of TC plantlets using carts developed.

7 Symposium on Light in Horticulture, Tsukuba, Japan Absorption spectrums of chlorophyll and phytochrome Chl.a 430, 662 nm Chl.b 453, 642 nm Phytoch.R 660 nm Phytoch.FR 730 nm 660+5nm 450+3nm 730+5nm 525+3nm Absorption spectrum W avelength of LEDs selected

8 Symposium on Light in Horticulture, Tsukuba, Japan Results and Discussion In 2008, development of –TC vessel with double lids –6 types of outer lids with different spectrums ( essential 6 ) –3 stages light intensity control In 2009 Q1,2 –6 spectrums + 2 with IR (essential 6+2)

9 Symposium on Light in Horticulture, Tsukuba, Japan Spectral contribution of Essential 6 + 2 14 B 401~500nm G 501~600nm R 601~700nm IR 701~800nm CW (5500 K) 26 %46262 WW (2700 K) 1045414 8R1B 1387 7R1G1B 13780 3R3B3IR 464212 6R 100 6B 100 6IR 100

10 Symposium on Light in Horticulture, Tsukuba, Japan Noted that 8R1B: ratio of number of LED chips used for the particular color R: 601~700 nm, G: 501~600 nm B: 401~500 nm, IR: 701~800 nm 8R1B: quantum ratio 87 : 13 7R1G1B: 80 : 7 : 13 3R3B3IR: 42 : 46 : 12

11 Symposium on Light in Horticulture, Tsukuba, Japan can be autoclaved transparent Poly-Carbonate 1. A TC vessel with double lids was developed. Inner lid outer lid

12 Symposium on Light in Horticulture, Tsukuba, Japan 2. Upper lid of the vessel contains 6 LED lamps, each with 6 chips

13 Symposium on Light in Horticulture, Tsukuba, Japan Distance from light source to plant was greatly reduced. 3.Elec. Consumption can be reduced. OthersThis research

14 Symposium on Light in Horticulture, Tsukuba, Japan 4.Light distribution highly uniform inside vessel Others This research

15 Symposium on Light in Horticulture, Tsukuba, Japan Double the number of layers compare with traditional bench 5.High space utilization vertically

16 Symposium on Light in Horticulture, Tsukuba, Japan 6.No pollution from light next to each other.

17 Symposium on Light in Horticulture, Tsukuba, Japan Side dividers prevent pollution from others

18 Symposium on Light in Horticulture, Tsukuba, Japan 7.Light quality adjustable Blue9R1BCW RedWW 8RGB Change the Lid, change the light quality

19 Symposium on Light in Horticulture, Tsukuba, Japan 8.Separate intensity control of each layer 8 x 5 = 40 vessels/layer x 8 layers = 320 vessels /cart

20 Symposium on Light in Horticulture, Tsukuba, Japan 9.Timer controlled D/N cycle

21 Symposium on Light in Horticulture, Tsukuba, Japan Plug and play 10.Easy to use

22 Symposium on Light in Horticulture, Tsukuba, Japan Each vessel has separate power input

23 Symposium on Light in Horticulture, Tsukuba, Japan 11.Great tool for Experiment Allowing 5 replicates x 64 treatments 5 8 8

24 Symposium on Light in Horticulture, Tsukuba, Japan Traditional lab.E-Light Density 138 vessels/m 2 4992 / (4.5 x 8) 482 vessels/m 2 320 / (1.07 x 0.62) Productivity ratio13.5 Power consumption 2560W (80 × 32) 1100W Power consumption ratio2.51 12.Highly efficient for mass production

25 Symposium on Light in Horticulture, Tsukuba, Japan Results and Discussion (cont.) In 2009 Q1,2 (cont.) –with 10 stages intensity control (0~60 micro-mol/m 2 /s) –Fine tune on light intensities of different lids

26 Symposium on Light in Horticulture, Tsukuba, Japan Giant E-Light v1 (2008 HortiFair) 3 stages intensity control

27 Symposium on Light in Horticulture, Tsukuba, Japan Giant E Light v2a (2009Q1) 10 stages intensity control 六種光蓋在十段光量控制下之比較 Intensities of 10 stages of LED Lids showing 6 types of LED lids (Essential 6)

28 Symposium on Light in Horticulture, Tsukuba, Japan Previous research (unpublished) E-Light prototype system was used Potato seedlings were grown under different light quantity and quality R B CW W LidsPAR All R 84 All B 86 CW 66 WW 75

29 Symposium on Light in Horticulture, Tsukuba, Japan Fine tune on light intensities of different lids 1 2543 6 7 F98 6 micro mole/m 2 /s increment per stage Giant E Light v2b (2009Q3)

30 Symposium on Light in Horticulture, Tsukuba, Japan Results and Discussion (cont.) In 2009 Q3 –20 spectrums, ‘Excellent 20’ –Design of a TC system for mass production using Giant E Light

31 Symposium on Light in Horticulture, Tsukuba, Japan Excellent 20

32 Symposium on Light in Horticulture, Tsukuba, Japan Mass production using 40 ft. Cargo container 38 carts/cargo, 12160 vessels/cargo 462 vessels/m 2

33 Symposium on Light in Horticulture, Tsukuba, Japan Results and Discussion (cont.) In 2009 Q3 (cont.) –Development of Mini E Light

34 Symposium on Light in Horticulture, Tsukuba, Japan Mini E-Light 2 layers –12 vessels per layer –24 vessels per cart 10 stages intensity control 2 timers for D/N cycle control

35 Symposium on Light in Horticulture, Tsukuba, Japan L66-W40-H80 cmL107-W62-H195 cm 110VAC/12A 220VAC/5.5A 110VAC/0.61A 220VAC/0.3A 40 /layer x 8 layers 12 /layer x 2 layers

36 Symposium on Light in Horticulture, Tsukuba, Japan Examples of using E-Light for photo-phyto related research 20 types of light quality 10 Intensity levels Timer(s) for D/N cycle

37 Symposium on Light in Horticulture, Tsukuba, Japan Examples of using E-Light for photo-phyto related research Group of Lids for research

38 Symposium on Light in Horticulture, Tsukuba, Japan Group of Lids for research: 3R3B in common 3R4B2IR 3R5B1IR 3R6B3R6B 3R3B3IR +3B +2B1IR +3IR +1B2IR

39 Symposium on Light in Horticulture, Tsukuba, Japan Group of Lids for research: 1R7B in common 2R7B2R7B1R8B1R8B 1R7B1IR +1B+1R +1IR

40 Symposium on Light in Horticulture, Tsukuba, Japan Study on the effects of R/B mix light Lid No.RB [16]9 [01]81 [04]63 [08]36 [12]27 [14]18 [17]9

41 Symposium on Light in Horticulture, Tsukuba, Japan Study on the effects of mono- wavelength vs. white LED light Lid No.RBIR [16] 9 [17] 9 [18] 9 [19] CW (5500 K) [20] WW (2700 K)

42 Symposium on Light in Horticulture, Tsukuba, Japan Study on the effects of R/B mix light with concurrent infrared (1IR or 2IR) Lid No,RBIR [03]711 [05]621 [06]531 [09]351 [13]261 [15]171 Lid No.RBIR [07]432 [10]342

43 Symposium on Light in Horticulture, Tsukuba, Japan Study on the effects of supplemental B, R or R/IR mix light with concurrent blue light (3B in common) Lid No. RBIR [17] 3 + 6 [04] 63 [06] 531 [07] 432 [11] 333

44 Symposium on Light in Horticulture, Tsukuba, Japan Study on the effects of supplemental red, infrared or green light with concurrent red and blue light (7R1B in common) Lid No.RBIRG [01] 7+11 [03] 71 1 [02] 71 1

45 Symposium on Light in Horticulture, Tsukuba, Japan Study on the effects of supplemental red, blue or blue/IR mix light with concurrent red light (3R in common) Lid No.RBIR [08]36 [09]351 [10]342 [11]333 [16]3 + 6

46 Symposium on Light in Horticulture, Tsukuba, Japan Study on the effects of B and IR with concurrent R/B mix light Lid No.RBIR [04]62+1 [05]621 [12]26+1 [13]261 [14]17+1 [15]171

47 Symposium on Light in Horticulture, Tsukuba, Japan Results and Discussion (cont.) In 2009 Q4 –Development of Home growth Light and Control E Light (From LED Lid to LED Panel)

48 Symposium on Light in Horticulture, Tsukuba, Japan Mini E-Light 0~60 umol/m 2 /s Home growth Light 0~180 umol/m 2 /s

49 Symposium on Light in Horticulture, Tsukuba, Japan Home-Growth-Light (0~180 umol/m2/s) LED panel made from LED Lids

50 Symposium on Light in Horticulture, Tsukuba, Japan Home Growth-Light Hardening of TC papaya plantlets Florescent Lamp LED (8R1B)

51 Symposium on Light in Horticulture, Tsukuba, Japan Controlled E-Light With Air conditioner and air curtain

52 Symposium on Light in Horticulture, Tsukuba, Japan LED panel made from LED Lids

53 Symposium on Light in Horticulture, Tsukuba, Japan Conclusions A TC vessel with double lid was developed. Outer lid equipped with LED lamps. Totally, 20 types of lids are available. Groups of lids for specific research are suggested.

54 Symposium on Light in Horticulture, Tsukuba, Japan Conclusions (cont.) Totally, 4 devices were developed: –Giant E-Light (using LED lid) –Mini E-Light (using LED lid) –Home Growth Light (using LED panel) –Controlled E-Light (using LED panel)

55 Symposium on Light in Horticulture, Tsukuba, Japan Conclusions A system using giant E-Light in cargo container for mass production of TC plantlets was proposed.

56 Symposium on Light in Horticulture, Tsukuba, Japan Conclusions (cont.) Posters 18P27, 18P28

57 Symposium on Light in Horticulture, Tsukuba, Japan 2008 Horti Fair

58 Symposium on Light in Horticulture, Tsukuba, Japan 58 51 2008 Horti Fair

59 Symposium on Light in Horticulture, Tsukuba, Japan 2009 Horti Fair

60 Symposium on Light in Horticulture, Tsukuba, Japan


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