Presentation on theme: "Physiological Variation in Vascular Reactivity of Breast Tissue over the Menstrual Cycle Demonstrated by Optical Tomography Katz MS 1, Hardin RE 1, Franco."— Presentation transcript:
Physiological Variation in Vascular Reactivity of Breast Tissue over the Menstrual Cycle Demonstrated by Optical Tomography Katz MS 1, Hardin RE 1, Franco NA 1, Smeraldi AD 2, Klemer DP 3, Schmitz CH 3, Pei Y 3 Graber HL 3, Barbour RL 3 1 Department of Surgery, SUNY Downstate Medical Center 2 Department of Vascular Surgery, Staten Island University Hospital 3 Department of Pathology, SUNY Downstate Medical Center Biomedical Optics 2004 Miami Beach, Florida April 14-17, 2004
Optical Tomography Group Technology Data Analysis Applications Breast Cancer Limb Brain NEC Time series imaging Time multiplexed DC illumination Fast reconstruction Image enhancement techniques Signal Analysis Quantitative Functional Imaging
Breast Cancer Cancer is the second most common cause of death. (22%) Breast cancer is the most common type of cancer in females. (211,300). The second most common cause of cancer death in females. (40,110) Most common cause of cancer death in African- American females.
Menstrual Cycle Estrogen Breast Enlargement Ductal Growth Progesterone Ductal Maturation Breast Swelling
Clinical Research Badwe et al: First study demonstrating correlation between long term survival and phase of menstruation. Follicular Phase Luteal Phase 3-12 d. after LMP.0-2, 13-32 d. after LMP. 54% 10 yr survival 84% Badwe RA, Gregory WM, Chaudary MA, Ricahrds MA, Bentley AE, Rubens RD, Fentimen IS, “Timing of Surgery During Menstrual Cycle and Survival of Premenopausal Women with Operable Breast Cancer”, Lancet, 1991,337;1:1261-1264.
DYNOT Breast Imager
Materials and Methods 5 Pre-menopausal healthy women were enrolled. Each women was measured 3-4 times during the menstrual cycle. Subject were followed for 1-3 months. Subjects were imaged for 1500 consecutive seconds. 500 second baseline (2 time points/second). Repeat Valsalva maneuver of 40mm Hg for 30-40 seconds. 200 second rest period. Subjects had each breast imaged separately using 25 source x 29 detector array. Data analysis consisted of applying various signal processing routines to the image time series.
Criteria for Selection of Provocation Effects entire breast. Repeatable. Serves as a useful discriminator.
Mean Time Series
Pre-Menopausal Day 1Day 12Day 19Day 28 Day 1Day 12Day 19Day 28 Oxy-Hemoglobin Deoxy-Hemoglobin
Pre-Menopausal Day 5Day 12Day 17Day 26 Day 1Day 12Day 19Day 28 Oxy-Hemoglobin Subject #1 Subject #2
Pre-Menopausal Day 1Day 12Day 19Day 28 Day 5Day 12Day 17Day 26 Deoxy-HemoglobinSubject #1 Subject #2
Post Menopausal Day 1Day 8Day 14Day 26 Oxy-Hemoglobin Deoxy-Hemoglobin
Percentage of Variance Percentage of Variance
Percentage of Variance Day 1Day 12Day 19Day 28 Oxygenated Hemoglobin Oxy-Hemoglobin
Phase 1 Day 1Day 12Day 19Day 28 % of SD Pairs 1- 0- 1- 0- 1- 0- 1- 0- 0 50 100 % of SD Pairs Oxy-Hemoglobin
Spatial Map of Onset of Maximal Response Right Breast P1 Left Breast P1 Right Breast N2 Left Breast N2 Day1 Day19 Day12
Conclusions Vascular response of breast to the Valsalva maneuver is mainly coherent and repeatable. Response profile to Valsalva maneuver varies over the menstrual cycle. Largest variation in profile is seen with deoxy-hemoglobin whereas largest variation in rate is seen with oxy-hemoglobin.