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Maureen N. Waswa, George M. Mwenda, Nancy K. Karanja, Paul Woomer and Fred Baijukya Effectiveness of Kenyan Rhizobia Strains.

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Presentation on theme: "Maureen N. Waswa, George M. Mwenda, Nancy K. Karanja, Paul Woomer and Fred Baijukya Effectiveness of Kenyan Rhizobia Strains."— Presentation transcript:

1 Maureen N. Waswa, George M. Mwenda, Nancy K. Karanja, Paul Woomer and Fred Baijukya Effectiveness of Kenyan Rhizobia Strains on Soybean

2 Justification Biodiversity and economic potential of African rhizobia is largely unexplored Potential exists for native rhizobia to outperform exotic commercial strains Objectives Identify elite native rhizobia for soybean Compare these rhizobia to standard industry strains

3 Steps in establishing rhizobia as elite strains Bio-prospecting and isolation Authentication and preliminary screening Effectiveness testing under greenhouse conditions Competitive abilities in potted soil Field testing 208 isolates 186 isolates 100 isolates 24 isolates 6 isolates

4 Greenhouse growth systems employed in this study Leonard jars with sand media Three liter pots with rhizobia-free vermiculite Growth pouches for MPN Three liter pots with site soils non-quantitative, abandoned as unnecessary clean controls, quantitative separation of isolates rapid estimation of native populations effective isolates tested for competitive abilities

5 Greenhouse effectiveness testing of 100 isolates in 3 liter pots with rhizobia-free vermiculite Clockwise: early N response (top), sterile irrigation system (upper right), effectiveness differences (lower right) and experimental overview (lower left).

6 Effectiveness testing under greenhouse conditions Strains were compared by Effective Index and then assigned to four categories ( USDA 110)

7 isolateEIHostSource AEZ NAK CowpeaCoastal plain NAK Eriosema sp.Coastal plain NAK SoybeanSemi-arid upland NAK CowpeaCoastal plain NAK soybeanSub-humid midland NAK soybeanSub-humid midland USDA USA (Industry standard) The best “candidate elite strains” emerging for greenhouse effectiveness testing

8 Clockwise: experimental overview (top right), MPN (lower left)and nodulation of test strain (lower right).

9 IsolatesEIHostSource AEZ NAK SoybeanSemi-arid upland NAK SoybeanSemi-arid upland NAK890.78SoybeanSemi-arid upland NAK840.84SoybeanSemi-arid upland NAK SoybeanSemi-arid upland NAK SoybeanSemi-arid upland USAD USA (Industry standard) The best promising elite strains emerging for greenhouse competitive ability testing

10 EI-Vermiculite 0.40 EI-Soil USDA110 Less competitive, highly effective. Less competitive, less effective. competitive, less effective. competitive, highly effective.

11 Competitive, highly effective Less competitive, highly effective Competitive, less effective Less competitive, less effective NAK115NAK9NAK12NAK10 NAK128NAK83NAK122NAK30 NAK89NAK135NAK84 NAK96NAK139NAK117 NAK127NAK146NAK144 NAK149NAK152NAK176 (SCcv) NAK176 (SB19)NAK160NAK179 (SCcv) NAK179 (SB19)NAK161 8%33%38%21%

12 Conclusion and recommendation For strains to be elite, they must be screened for genetic stability, satisfactory growth and survival under inoculant manufacturing conditions. Preliminary testing of promising native strains have been done and further testing continues at the field with six promising native isolates. Competitive, highly effective and less competitive, highly effective: field testing should be done in different soils and environment. Isolates NAK179 and NAK176 performed well on promiscuous but not on specific soybean: Different inoculants maybe required for different soybean genotypes.

13 Test strain + Nitrogen - Nitrogen

14

15 ACKNOWLEGEMENT CIAT-TSBF through N2 AFRICA for funding the study. University of Nairobi for hosting the study. Organizers of ISFM conference.

16 THANK YOU


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