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Real-time Monitoring technology of Mass spectrometry Speaker : Achong.

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Presentation on theme: "Real-time Monitoring technology of Mass spectrometry Speaker : Achong."— Presentation transcript:

1 Real-time Monitoring technology of Mass spectrometry Speaker : Achong

2 Outline IMS PTR-MS SIFT-MS Application of IMS IMS-TOF IMS-IMS-TOF Application of PTR-MS PIT-MS

3 Real-time Monitoring Mass spectrometry Environmental science Biochemical and medical diagnosis Workplace safety Security check

4 EI source Analysis of atmospheric air is especially challenging using electron ionization mass spectrometry, because the gas loading of the ion source by N 2 and O 2 (as well as water vapor) can be excessive. 70 ev

5 Real-time Monitoring MS Soft chemical ionization Measurement in Real-Time Without pre-concentration system

6 I. IMS (ion mobility spectrometer) Ion mobility mass spectrometry (Eiceman,1991; Baumbach, 2001) Analytical Chemistry, A390-A397, November 1, 2004

7 Ion mobility (K) Journal of Bimolecular Technique, Vol.13, Issue 2, June, 2002

8 Ion Mobility-Mass Spectroscopy Witt & Bowers: JACS. 2000, 122, 3458.

9 Ion mobility mass spectrometry Advantages: Multiform ion source could be applied. Some isomer could be separated. Real-time measurement Disadvantages: Lower mass resolution Lower sensitivity to allow accurate analysis of complex mixtures. Standard is requisite

10 II. PTR-MS (proton-transfer reaction) Proton-transfer reaction-mass spectrometry (Lagg A, Taucher J, Hansel A, Lindinger W )

11 proton-transfer reaction H 3 O + + R → RH + + H 2 0 [RH + ] = [H 3 O + ] o (1 - e -k[R]t ) = [H ] o [R]kt J. Mass Spectrom Ion Processes, 173 (1998)

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14 PTR mass spectrometry Advantages: Soft ionization and low matrix effect Real-time measurement Low limit of detection (0.5 ppbv for 1-min measurements) Disadvantages: A large number of VOCs could not be identified and quantified.

15 III. SIFT-MS (selected ion flow tube) Adams NG, Smith D Spanel P,Smith D

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17 SIFT mass spectrometry Advantages: application of multiple reagent ions (H 3 O +, NO + and O 2 + ) quantification in real-time discriminate isomers in Real-Time Disadvantages: The kinetic parameters of the reagent ion and the analyte ion must be known. Selected ion flow tube mass spectrometry for no-line trace gas analysis David Smith and Patrik Spanel Reference : Mass Spectrometry Reviews, 2005, 24, 661– 700

18 Application of IMS IMS/TOF IMS/IMS/TOF

19 Ion Mobility/TOFMS Anal. Chem. 2003, 75,

20 Pulse sequence of IMS & TOF Anal. Chem. 2003, 75,

21 example spectrum Analytical Chemistry, 1998 Vol. 70, No. 11, June 1,

22 Anal. Chem. 2003, 75,

23 collision-induced dissociation

24 IMS/IMS/TOF Anal. Chem. 2006, 78,

25 operational mode A Anal. Chem. 2006, 78,

26 operational mode B Anal. Chem. 2006, 78,

27 operational mode C Anal. Chem. 2006, 78,

28 Application of PTRMS PIT-MS

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30 PIT-MS proton-transfer ion trap-mass spectrometry J Am Soc Mass Spectrom 2005, 16, 1316–1324

31 CID process on ion-trap MS

32 calibration measurement of the PIT-MS J Am Soc Mass Spectrom 2005, 16, 1316–1324

33 single CID measurement of m/z 59 ambient airpure acetonepure propanal J Am Soc Mass Spectrom 2005, 16, 1316–1324

34 CID of propanal and acetone m/z=59m/z=41m/z=31 J Am Soc Mass Spectrom 2005, 16, 1316–1324


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