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Supplementary Figure 1 – Toh et. al. 0 hr 12 hr24 hr 24 hr + PurvATaxol: 24 hr + MG132 24 hr + Roscovitine 24 hr + R0-3066 MPM2levels Thymidine block Thymidine.

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Presentation on theme: "Supplementary Figure 1 – Toh et. al. 0 hr 12 hr24 hr 24 hr + PurvATaxol: 24 hr + MG132 24 hr + Roscovitine 24 hr + R0-3066 MPM2levels Thymidine block Thymidine."— Presentation transcript:

1 Supplementary Figure 1 – Toh et. al. 0 hr 12 hr24 hr 24 hr + PurvATaxol: 24 hr + MG hr + Roscovitine 24 hr + R MPM2levels Thymidine block Thymidine Wash-out; +/-Taxol; +/-R /-MG132; +/- Roscovitine; +/- Purv-A Time (hrs): Cell Cycle Phase: Asyn. G1/S Mitosis Collection B UTPurATaxolPurA + Taxol % PI stained cells A

2 2N 4N Taxol + Purv A Taxol + MG132 Taxol + R PurvAMG132R UT (0 hrs) UT (24hrs) Roscovitine Taxol + Roscovitine Thymidine block Thymidine Wash-out; +/-Taxol; +/-R /-MG132; +/- Roscovitine; +/- Purv-A Time (hrs): Cell Cycle Phase: Asyn. G1/S Mitosis Collection Taxol (24hrs) Supplementary Figure 2 – Toh et. al. 2N 4N

3 Released in CDDP from Thymidine-block Thymidine-blocked + CDDP Released in DMEM from Thymidine-block M1 Supplementary Figure 3 – Toh et. al. 1.9%1.6% 16.7%15.8% 14.8% 15.4% 2N 4N

4 H1299 A549 U2OS Controlsi73.1si73.2si73.3si73.4 Controlsi73.1si73.2si73.3si73.4 p73 gapdh Controlsi73.1si73.2si73.3si73.4 siRNA: A549U2OS Controlp73.1p73.2p73.3p Controlp73.1p73.2p73.3p73.4 UT Taxol ControlsiRNA:p73.1p73.2p73.3p73.4 % cleaved caspase-3 H1299 B A Supplementary Figure 4 – Toh et. al.

5 : Time (hrs) WT p53 -/- p73 -/- 2N 4N 2N 4N 8N 2N 4N Supplementary Figure 5 – Toh et. al.

6 Control siRNA 1 Control siRNA 2 PLK1 siRNA 1 PLK1 siRNA 2 Supplementary Figure 6 – Toh et. al. 2N 4N

7 Asynchronous Thymidine-blocked and released UTMG132UT MG %3.1%14.8%16.4% 4.1% 4.7% 39.6% 40.5% Supplementary Figure 7 – Toh et. al. PI UT MG132

8 pcDNA p73  DNp73  % cleaved caspase-3 UT Taxol pcDNA p73  p73DD % cleaved Caspase-3 UT Taxol B A Supplementary Figure 8 – Toh et. al.


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