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Published byAlexia Wells Modified over 9 years ago
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Microbiology and HACCP For Surimi Seafood
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Surimi Seafood Microbiology Production and storage Microbial quality Microbial safety Pasteurization studies Non-thermal processing HACCP Microbial standards Packaging Fermented products Rapid test kits
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Microbiology of Surimi During Production and Storage
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Microbial Quality and Safety of Surimi Seafood Depends On: Microbial load in the raw surimi Microbial load in ingredients Processing time/temperature abuse Equipment sanitation Employee hygiene
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a = surimi b = surimi plus ingredients c = first cook/rope formation d = color addition e = second cook f = flaking or chopping g = packaging h = “pasteurization”
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a = surimi b = surimi plus ingredients c = first cook/rope formation d = color addition e = second cook f = flaking or chopping g = packaging h = pasteurization
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a = surimi b = surimi plus ingredients c = first cook/rope formation d = color addition e = second cook f = flaking or chopping g = packaging h = pasteurization
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Equipment Sanitation Belt conveyor1,800,000/cm 2 Container of mixed paste25,000/cm 2 Inner wall of mixer540/cm 2
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Surimi Seafood Shelf Life Days at:15 o C10 o C5 o C0 o C 59 o F50 o F41 o F32 o F Surimi crab legs414>28>28 Flaked surimi crab meat<3<4<714
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Microbial Quality of Surimi Seafood: Bacillus Species and Other Gram-positive Bacteria
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Pasteurization Has no effect on bacterial spores, i.e., Clostridium and Bacillus species Bacillus species are prime spoilers in air- packaged surimi seafood
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Microbial Safety of Surimi Seafood: Listeria monocytogenes, Clostridium botulinum and Other Bacteria
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L. monocytogenes 1988 surimi survey found 29% of samples positive 1988 U.S. Class I recall of imitation crab meat produced in Japan and distributed in three states
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Table 1. Surimi Seafood Recalls Due to L. monocytogenes Imitation crab meat products7/28/99 Imitation crab spread9/17/97 Imitation king crab legs8/6/97 Imitation crab meat chunks6/12/96 Imitation crab meat salad9/30/92 Seafood salad7/2/92
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Clostridium botulinum Strict anaerobe Grows above 38°F No instances reported from surimi seafood C. botulinum type E and nonproteolytic types B and F are the target bacteria for FDA’s pasteurization processes – 6-D process = 90°C (194°F) for 10 minutes
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Generation Times (Hours) for Pathogens in Surimi Seafood 15 o C10 o C5 o C0 o C 59 o F50 o F41 o F32 o F Aeromonas hydrophila1848194- Salmonella species1134-- Staphylococcus aureus2946-- Yersinia enterocolitica112677166
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Pasteurization Studies for Surimi Seafood Inoculated pack studies with Enterococcus faecium – 93°C (199.4°F) 5 minutes – 85°C (185°F) for 15 minutes – 75°C (167°F) for 15 minutes Yielded 6 log reduction of E. faecium – Process also effective against C. botulinum Type E (85 and 93°C), L. monocytogenes, enteropathogenic E. coli, Salmonella, Yersinia entercolitica, and Vibrio Ineffective against C. botulinum Type B
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Pasteurization Studies for Surimi Seafood TemperatureTimeD-Values (min.)L.m.C.b.(E)C.b.(B) 93°C (199.4°F) 516,200520.7 85°C (185°F) 155,400240.6 75°C (167°F) 153502.20.1
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Microbiological Implications of Novel Surimi Processing Technologies High pressure – 200-400 MPa – Effective against Vibrio, Listeria, Salmonella – Ineffective against pressure resistant species and spore forming Bacillus and Clostridium species Electron beam – Untested
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HACCP and Surimi Seafood Preventive system of food safety control Based on – Identified hazards – Critical control points – Monitoring records
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Metal Fragments Hard or sharp objects >7 mm in size are a potential hazard from laceration, perforation wound, and secondary infections Hard or sharp objects <7 mm in size are a possible hazard for high risk (e.g., infants, elderly) individuals Controls can include frequent inspections of cutting, portioning, blending, or other mechanical equipment for damage, or use of a metal detector
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Pasteurization Eliminates targeted pathogenic bacteria and also extends product shelf life Pathogens in packaged products indicates inadequate pasteurization, or post pasteurization contamination, and time/temperature abuse Critical aspects: temperature of heating medium, length of pasteurization cycle, package thickness, package integrity, product formulation, and microbial quality of the cooling medium
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Cooling Rapid cooling prevents growth from Bacillus and Clostridium 60°C (140°F) to 21.1°C (70°F) in 2 hours To 4.4°C (40°F) within another 4 hours
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Refrigerated Storage Refrigerated storage below 4.4°C (40°F) prevents growth of pathogens
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Microbial Recommendations for Ready-to-eat Seafood ncmM Aerobic plate count5210 5 10 6 E. coli5111500 S. aureus5010 3 - V. parahaemolyticus10110 2 10 3
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FDA Tolerance Levels: Vacuum Packaged Ready-to-eat Seafood C. botulinumPresence of cells, Enteropathogenic E. coli10 3 /g L. monocytogenesPresence SalmonellaPresence S. aureus10 4 /g or toxin positive V. choleraePresence V. parahaemolyticus10 4 /g V. vulnificusPresence
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Microbiological Considerations in Packaging of Surimi Seafood Nitrogen and carbon dioxide packaging reduce fat and pigment oxidation and reduce spoilage bacterial growth Aseptic packaging requires a sterile product and may not be feasible
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New Surimi Seafood Products Utilizing Fermentative Bacteria Fermented pollock kamaboko – pH 4.5 – Sour tasting Fermented chum salmon surimi Sensory studies have not been conducted
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Reliability and Efficacy of Rapid Microbiological Procedures Traditional methods – APC with petri dishes or PetriFilm Rapid test kits for L. monocytogenes – 1-2 days instead of 5-7 days
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Conclusion Surimi seafood Products are ready-to-eat – Safety and quality concerns remain – HACCP can help ensure a safe product The use of the term “pasteurization” should be discouraged or the process needs to be further studied
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