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Plant Cell Biology Wouter Kohlen 0427705 Auxin and Fruit Set The role Solanum lycopersicum Auxin Response Factor 1 in the initiation of fruit development.

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Presentation on theme: "Plant Cell Biology Wouter Kohlen 0427705 Auxin and Fruit Set The role Solanum lycopersicum Auxin Response Factor 1 in the initiation of fruit development."— Presentation transcript:

1 Plant Cell Biology Wouter Kohlen Auxin and Fruit Set The role Solanum lycopersicum Auxin Response Factor 1 in the initiation of fruit development

2 Plant Cell Biology Wouter Kohlen Content Introduction Gibberellins Pollen tube growth cDNA-AFLP

3 Plant Cell Biology Wouter Kohlen Auxin Cell elongation Embryo patterning Apical dominance Vascular differentiation Abscission Tropisms Root morphology Fruit set Introduction

4 Plant Cell Biology Wouter Kohlen Auxin Introduction Gillapsy;1993

5 Plant Cell Biology Wouter Kohlen Introduction Woodward; 2005

6 Plant Cell Biology Wouter Kohlen Wt79.8 Introduction

7 Plant Cell Biology Wouter Kohlen Wt79 Introduction

8 Plant Cell Biology Wouter Kohlen Wt 79 Introduction

9 Plant Cell Biology Wouter Kohlen Gibberellins Samples Wt 3 days poll Wt 3 days unpoll pathway

10 Plant Cell Biology Wouter Kohlen Gibberellins

11 Plant Cell Biology Wouter Kohlen Gibberellins Samples Wt day -2 Wt day 5 poll Wt day 5 unpoll 79.8 day day 5 To do… Welke genen?

12 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x ♀Wt x ♂Wt

13 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x ♀Wt x ♂Wt

14 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x ♀Wt x ♂Wt

15 Plant Cell Biology Wouter Kohlen Pollen tube growth 100x ♀Wt x ♂Wt

16 Plant Cell Biology Wouter Kohlen Pollen tube growth 250x ♀Wt x ♂Wt

17 Plant Cell Biology Wouter Kohlen Pollen tube growth 100x ♀79.8 x ♂79.8

18 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x ♀79.8 x ♂79.8

19 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x ♀79.8 x ♂79.8

20 Plant Cell Biology Wouter Kohlen Pollen tube growth 100x ♀79.8 x ♂79.8

21 Plant Cell Biology Wouter Kohlen Pollen tube growth 250x ♀79.8 x ♂79.8

22 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

23 Plant Cell Biology Wouter Kohlen Pollen tube growth 100x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

24 Plant Cell Biology Wouter Kohlen Pollen tube growth 250x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

25 Plant Cell Biology Wouter Kohlen Pollen tube growth 100x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

26 Plant Cell Biology Wouter Kohlen Pollen tube growth 250x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

27 Plant Cell Biology Wouter Kohlen Pollen tube growth 100x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

28 Plant Cell Biology Wouter Kohlen Pollen tube growth 250x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

29 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x 79.8

30 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x 100x 250x400x 79.8

31 Plant Cell Biology Wouter Kohlen Pollen tube growth 40x 100x 250x400x 79.8 To be continued

32 Plant Cell Biology Wouter Kohlen cDNA-AFLP Samples 79.8 D0 emasc. Ovaries 79.8 D0 emasc. Walls 79.8 D5 emasc. ovaries 79.8 D5 emasc. walls 79.8 D0 not emasc. ovaries 79.8 D0 not emasc. walls 79.8 D5 not emasc. ovaries 79.8 D5 not emasc. Walls wt D0 unpoll ovaries wt D0 unpoll walls wt D5 unpoll ovaries wt D5 unpoll walls wt D5 poll ovaries wt D5 poll walls D1 unpoll ovaries D1 unpoll walls D3 unpoll ovaries D3 unpoll walls D1 poll ovaries D1 poll walls D3 poll ovaries D3 poll walls wt D1 unpoll ovaries wt D1 unpoll walls wt D3 unpoll ovaries wt D3 poll walls wt D1 poll ovaries wt D1 poll walls wt D3 poll ovaries wt D3 poll walls 159 ovaries 159 walls

33 Plant Cell Biology Wouter Kohlen cDNA-AFLP Order on gel wt D0 unpoll ovaries wt D1 unpoll ovaries wt D3 unpoll ovaries wt D5 unpoll ovaries wt D1 poll ovaries wt D3 poll ovaries wt D5 poll ovaries wt D0 unpoll walls wt D1 unpoll walls wt D3 unpoll walls wt D5 unpoll walls wt D1 poll walls wt D3 Poll walls wt D5 poll walls D1 unpoll ovaries D3 unpoll ovaries D1 poll ovaries D3 poll ovaries D1 unpoll walls D3 unpoll walls D1 poll walls D3 poll walls 79.8 D0 emasc. ovaries 79.8 D5 emasc. ovaries 79.8 D0 not emasc. ovaries 79.8 D5 not emasc. ovaries 79.8 D0 emasc. walls 79.8 D5 emasc. walls 79.8 D0 not emasc. walls 79.8 D5 not emasc. Walls 159 ovaries 159 walls

34 Plant Cell Biology Wouter Kohlen cDNA-AFLP Primer combination 69

35 Plant Cell Biology Wouter Kohlen cDNA-AFLP Wt Ovules Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination Unpoll

36 Plant Cell Biology Wouter Kohlen Ovules cDNA-AFLP Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 69 Wt

37 Plant Cell Biology Wouter Kohlen Ovules cDNA-AFLP Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 69 Wt

38 Plant Cell Biology Wouter Kohlen cDNA-AFLP Ovaries Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 143 Wt

39 Plant Cell Biology Wouter Kohlen Ovules cDNA-AFLP Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 69 Wt ARF9

40 Plant Cell Biology Wouter Kohlen Primer combination 166


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