1. Plant Cell Biology Wouter Kohlen 0427705 Auxin and Fruit Set The role Solanum lycopersicum Auxin Response Factor 1 in the initiation of fruit development

2. Plant Cell Biology Wouter Kohlen 0427705 Content Introduction Gibberellins Pollen tube growth cDNA-AFLP

3. Plant Cell Biology Wouter Kohlen 0427705 Auxin Cell elongation Embryo patterning Apical dominance Vascular differentiation Abscission Tropisms Root morphology Fruit set Introduction

4. Plant Cell Biology Wouter Kohlen 0427705 Auxin Introduction Gillapsy;1993

5. Plant Cell Biology Wouter Kohlen 0427705 Introduction Woodward; 2005

6. Plant Cell Biology Wouter Kohlen 0427705 Wt79.8 Introduction

7. Plant Cell Biology Wouter Kohlen 0427705 Wt79 Introduction

8. Plant Cell Biology Wouter Kohlen 0427705 Wt 79 Introduction

9. Plant Cell Biology Wouter Kohlen 0427705 Gibberellins Samples Wt 3 days poll Wt 3 days unpoll pathway

10. Plant Cell Biology Wouter Kohlen 0427705 Gibberellins

11. Plant Cell Biology Wouter Kohlen 0427705 Gibberellins Samples Wt day -2 Wt day 5 poll Wt day 5 unpoll 79.8 day -2 79.8 day 5 To do… Welke genen?

12. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x ♀Wt x ♂Wt

13. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x ♀Wt x ♂Wt

14. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x ♀Wt x ♂Wt

15. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 100x ♀Wt x ♂Wt

16. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 250x ♀Wt x ♂Wt

17. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 100x ♀79.8 x ♂79.8

18. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x ♀79.8 x ♂79.8

19. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x ♀79.8 x ♂79.8

20. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 100x ♀79.8 x ♂79.8

21. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 250x ♀79.8 x ♂79.8

22. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

23. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 100x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

24. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 250x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

25. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 100x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

26. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 250x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

27. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 100x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

28. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 250x 40x ♀79.8 x ♂wt 40x ♀wt x ♂79.8

29. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x 79.8

30. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x 100x 250x400x 79.8

31. Plant Cell Biology Wouter Kohlen 0427705 Pollen tube growth 40x 100x 250x400x 79.8 To be continued

32. Plant Cell Biology Wouter Kohlen 0427705 cDNA-AFLP Samples 79.8 D0 emasc. Ovaries 79.8 D0 emasc. Walls 79.8 D5 emasc. ovaries 79.8 D5 emasc. walls 79.8 D0 not emasc. ovaries 79.8 D0 not emasc. walls 79.8 D5 not emasc. ovaries 79.8 D5 not emasc. Walls wt D0 unpoll ovaries wt D0 unpoll walls wt D5 unpoll ovaries wt D5 unpoll walls wt D5 poll ovaries wt D5 poll walls 166.17 D1 unpoll ovaries 166.17 D1 unpoll walls 166.17 D3 unpoll ovaries 166.17 D3 unpoll walls 166.17 D1 poll ovaries 166.17 D1 poll walls 166.17 D3 poll ovaries 166.17 D3 poll walls wt D1 unpoll ovaries wt D1 unpoll walls wt D3 unpoll ovaries wt D3 poll walls wt D1 poll ovaries wt D1 poll walls wt D3 poll ovaries wt D3 poll walls 159 ovaries 159 walls

33. Plant Cell Biology Wouter Kohlen 0427705 cDNA-AFLP Order on gel wt D0 unpoll ovaries wt D1 unpoll ovaries wt D3 unpoll ovaries wt D5 unpoll ovaries wt D1 poll ovaries wt D3 poll ovaries wt D5 poll ovaries wt D0 unpoll walls wt D1 unpoll walls wt D3 unpoll walls wt D5 unpoll walls wt D1 poll walls wt D3 Poll walls wt D5 poll walls 166.17 D1 unpoll ovaries 166.17 D3 unpoll ovaries 166.17 D1 poll ovaries 166.17 D3 poll ovaries 166.17 D1 unpoll walls 166.17 D3 unpoll walls 166.17 D1 poll walls 166.17 D3 poll walls 79.8 D0 emasc. ovaries 79.8 D5 emasc. ovaries 79.8 D0 not emasc. ovaries 79.8 D5 not emasc. ovaries 79.8 D0 emasc. walls 79.8 D5 emasc. walls 79.8 D0 not emasc. walls 79.8 D5 not emasc. Walls 159 ovaries 159 walls

34. Plant Cell Biology Wouter Kohlen 0427705 cDNA-AFLP Primer combination 69

35. Plant Cell Biology Wouter Kohlen 0427705 cDNA-AFLP Wt 166.17 79.8 159 Ovules Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 69 0 1 3 5 1 3 0 5 1 3 5 Unpoll

36. Plant Cell Biology Wouter Kohlen 0427705 Ovules cDNA-AFLP Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 69 Wt 166.17 79.8 159 0 1 3 5 1 3 0 5 1 3 5

37. Plant Cell Biology Wouter Kohlen 0427705 Ovules cDNA-AFLP Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 69 Wt 166.17 79.8 159 0 1 3 5 1 3 0 5 1 3 5

38. Plant Cell Biology Wouter Kohlen 0427705 cDNA-AFLP Ovaries Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 143 Wt 166.17 79.8 159 0 1 3 5 1 3 0 5 1 3 5

39. Plant Cell Biology Wouter Kohlen 0427705 Ovules cDNA-AFLP Walls Unpoll Poll Walls Unpoll Poll E NE O W E NE Primer combination 69 Wt 166.17 79.8 159 0 1 3 5 1 3 0 5 1 3 5 ARF9

40. Plant Cell Biology Wouter Kohlen 0427705 Primer combination 166