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Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman “… the art and science of multiplying.

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Presentation on theme: "Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman “… the art and science of multiplying."— Presentation transcript:

1 Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman Teknik & Aplikasi Kuljar Dalam Produksi & Kualitas Tanaman “… the art and science of multiplying plants in vitro.” Dr. Sukendah, MSc

2 Pembentukan Benih Sintetik Artificial seed coat Somatic embryo Artificial endosperm Benih sintetik (Synthetic/Artificial Seed) adalah produksi benih dari embrio somatik yang dibungkus dalam mantel tiruan

3 -MS medium + phytohormone + carbon sources.carbon source -Sodium alginate was added to the medium and mixed well to make a homogenous solution. -The explants were taken and dipped into the alginate solution. -Explants dipped in alginate solution were taken up by a forcep and placed to the beaker of CaCl 2 -Each explant then became a hard ball encoded by alginate. Teknik Pembuatan Benih Sintetik

4 Bahan Benih: Embrio Somatik Sodium alginate Calcium cloride Seed Coat Seed Artificial Seed 3% Sodium alginate + MS free calcium salt solution 2 75 mM CaCl2.2H2O Pembentukan seedcoat

5 Haploid Plant Production  Embryo rescue of interspecific crosses Creation of alloploids ( e.g. triticale) Bulbosum method  Anther culture/Microspore culture Culturing of Anthers or Pollen grains (microspores) Derive a mature plant from a single microspore  Ovule culture Culturing of unfertilized ovules (macrospores) Sometimes “trick” ovule into thinking it has been fertilized

6 Anther/Microspore Culture

7 Anther/Microspore Culture Factors  Genotype As with all tissue culture techniques  Growth of mother plant Usually requires optimum growing conditions  Correct stage of pollen development Need to be able to switch pollen development from gametogenesis to embryogenesis  Pretreatment of anthers Cold or heat have both been effective  Culture media Additives, Agar vs. ‘Floating’

8 What do you do with the haploid?  Weak, sterile plant  Usually want to double the chromosomes, creating a dihaploid plant with normal growth & fertility  Chromosomes can be doubled by Colchicine treatment Spontaneous doubling ○ Tends to occur in all haploids at varying levels ○ Many systems rely on it, using visual observation to detect spontaneous dihaploids ○ Can be confirmed using flow cytometry

9 Protoplast Created by degrading the cell wall using enzymes

10 Protoplast fusion  Protoplasts are made from two species that you want to cross  The membranes are made to fuse osmotic shock, electrical current, virus  Regenerate the hybrid fusion product  Contain genome from both organisms  Very, very difficult

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