Presentation on theme: "Investigation of diabetic patients with chronic ulcers in the lower limb Non-healing wounds >2 months Biopsi from wond margin for biomarker analysis, stored."— Presentation transcript:
Investigation of diabetic patients with chronic ulcers in the lower limb Non-healing wounds >2 months Biopsi from wond margin for biomarker analysis, stored at -82 C Biopsi from wond margin for biomarker analysis, stored at -82 C Superficial and deep biopsi from wound for bacterial culture 0 Wound dressings (hydrofiber) 3 times/week Days 2818 Study design Routin blood samples Digital image of wound Toe pressure Measurement of wound area (Visitrac) and wound depth Weeks Venous sample for biomarker analysis At every visit 20
Methods Diabetic patients (type I & II) suffering from a chronic ulcer in the lower limb (failed to heal >2 months after debut) were included in the study. This presentation gives preliminary data from biomarker analyses in 3 patients who failed to heal and 2 patients who healed completely within 15 weeks of observation. Venous blood samples were taken at every visit in pre-chilled tubes (EDTA) and centrifuged in a refrigerated centrifuge, aliquoted to cryotubes and stored at -85 o C until analysis. Data are given as mean values. Results are preliminary.
Methods Biochip Multi-spot ECL Technology (MesoscaleDiscovery, USA) This high resolution technique is based on electrochemical stimulation of the electrode surface of multi-spot plates. Antibodies directed against specific biomarkers are attached to a pure non-chargeable carbon surface which eliminates disturbing background activity. Bioluminescence is induced by current and measured by a CCL-camera.
Fibroblast Growth Factor The FGF family is composed of 23 members. We analysed FGF-2 or basicFGF. Keratinocytes Mast cells Chondrocytes Smooth muscle cells Endothelial cells Fibroblasts basicFGF Tissue remodelling Re-epithelialization Formation of granulation tissue
VEGFR-1 is required for organization of blood vessels. Released upon injury from activated platelets and from macrophages during wound healing Non-healers