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Oxygen Requirements 1. Aerobes 2. Anaerobes 3. Facultative 4. Microaerophilic.

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Presentation on theme: "Oxygen Requirements 1. Aerobes 2. Anaerobes 3. Facultative 4. Microaerophilic."— Presentation transcript:

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2 Oxygen Requirements 1. Aerobes 2. Anaerobes 3. Facultative 4. Microaerophilic

3 Agar Stabs & Agar Shakes 1. Liquify 6 NA Deeps in boiling water 2. Boil for 10 minutes to drive out dissolved oxygen 3. Allow 3 NA Deeps to cool and solidify Agar Stabs 4. Temper 3 NA Deeps in water bath at 55 degrees C Agar Shakes

4 Agar Stabs 1. Single stab with Inoculating needle – straight down, straight back up 1. Micrococcus luteus 2. Staphylococcus epidermidis 3. Clostridium sporogenes Clostridium rubrum Clostridium butrycum

5 Agar Shakes 1. Inoculate with Inoculating Loop - “shake” test tube so bacteria are equally distributed 1. Micrococcus luteus 2. Staphylococcus epidermidis 3. Clostridium sporogenes Clostridium rubrum Clostridium butrycum

6 Agar Stab Aerobic

7 Agar Stab Anaerobic

8 Agar Stab Facultative

9 Agar Stab Microaerophilic

10 Fluid Thioglycollate Broths (FTB) Sodium thioglycollate Binds to and removes free oxygen Resazurin Anaerobic indicator O2 - PINK No O2 - Clear

11 Oxygen Reqirements

12 Aerobic Microaerophilic Anaerobic


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