Presentation is loading. Please wait.

Presentation is loading. Please wait.

Microbiology Test Results: What do they mean?

Similar presentations

Presentation on theme: "Microbiology Test Results: What do they mean?"— Presentation transcript:

1 Microbiology Test Results: What do they mean?
Christie Massen M.S., MLS(ASCP) Microbiologist III – QA/CLIA Coordinator North Dakota Department of Health Division of Laboratory Services - Microbiology

2 Objectives Upon completion of this presentation, participants will be able to: Summarize various microbiological testing methods. List the components of a laboratory report. Interpret various microbiology test results. Summarize antibiotic susceptibility testing and results. Compare various serological test results.

3 Infectious Diseases Bacterial Viral Fungal Parasitic

4 Supporting Laboratory Results
Viral Identification of virus Normal or Decreased WBC Count Increased Lymphocyte Count Parasitic Identification of parasite Increased Eosinophil Count Bacterial Identification of bacteria Increased WBC Count Increased Neutrophil Count Left-shift in Neutrophil Cell Line Increased CRP Increased Sed-Rate Fungal KOH Prep Test Identification of fungal elements

5 Laboratory Reports

6 Bacterial Culture Grow and isolate bacteria Various media
Identify a pathogen Pros Identify the isolate Very specific Cons Lengthy Normal flora

7 Biochemical Testing

8 Identification

9 Antibiotic Susceptibility Testing
Determine antibiotic susceptibility or resistance

10 Antibiotic Susceptibility Testing

11 Case 1

12 Case 1: Report

13 Case 2

14 Case 2: Report

15 Modified Hodge Test Purpose: Interpretation:
Detection of carbapenemase production in identified organisms Interpretation: Positive Clover leaf-like indentation along the streak line Negative No growth along the streak line

16 Case 3

17 Case 3: Report

18 Virology

19 Virology Culture Cytopathic Effect (CPE) Culture Molecular

20 Case 4

21 Case 4: Report

22 Parasitology & Mycology
Direct Examination Macroscopic and Microscopic

23 Molecular Testing DNA Probes DNA sequencing
Nucleic acid sequences (16s) Whole genome sequencing Nucleic Acid Amplification Testing (NAAT) Polymerase Chain Reaction (PCR) Ligase Chain Reaction (LCR) Transcription Mediated Amplification (TMA)

24 PCR Developed in 1985 First NAA method How it works:
DNA Polymerase replicates the target DNA molecules RNA can be replicated Heat and cool cycles utilized DNA Primers hybridize the target and initiate polymerization of new DNA Molecules End product: Billions of copies of the target DNA Read for presence of DNA target


26 Immunology Test Methods
Detects: Antigens Antibodies Test Methods: Agglutination Nephlometry Double Diffusion Radial Immunodiffusion Electrophoresis Western Blot Flocculation ELISA Immunoflorescence assay Radioimmunoassay

27 Antibody Review

28 Antibody Review

29 Case 5

30 Case 5: Report

31 Case 6

32 Case 6: Report

33 Case 7

34 Case 7: Report

35 Case 8

36 Vaccine Preventable Disease Case 8: Report

37 Questions?

38 References Kiser, K.M., Payne, W.C., & Taff, T.A. (2011). Clinical Laboratory Microbiology: A Practical Approach. Upper Saddle River, NJ: Pearson Education. Amjad, A., Mirza, I.A., Zia, F. (2011). Modified Hodge test: A simple and effective test for detection of carbapenemase production. Iran Journal of Microbiology. Retrieved from: New Directions in Molecular Testing: An Educational Guide for Clinical Laboratory Professionals. Gen-Probe Inc. Retrieved from:

Download ppt "Microbiology Test Results: What do they mean?"

Similar presentations

Ads by Google