Presentation on theme: "Microbiology Test Results: What do they mean?"— Presentation transcript:
1Microbiology Test Results: What do they mean? Christie Massen M.S., MLS(ASCP)Microbiologist III – QA/CLIA CoordinatorNorth Dakota Department of HealthDivision of Laboratory Services - Microbiology
2ObjectivesUpon completion of this presentation, participants will be able to:Summarize various microbiological testing methods.List the components of a laboratory report.Interpret various microbiology test results.Summarize antibiotic susceptibility testing and results.Compare various serological test results.
4Supporting Laboratory Results ViralIdentification of virusNormal or Decreased WBC CountIncreased Lymphocyte CountParasiticIdentification of parasiteIncreased Eosinophil CountBacterialIdentification of bacteriaIncreased WBC CountIncreased Neutrophil CountLeft-shift in Neutrophil Cell LineIncreased CRPIncreased Sed-RateFungalKOH Prep TestIdentification of fungal elements
15Modified Hodge Test Purpose: Interpretation: Detection of carbapenemase production in identified organismsInterpretation:PositiveClover leaf-like indentationalong the streak lineNegativeNo growth along the streak line
24PCR Developed in 1985 First NAA method How it works: DNA Polymerase replicates the target DNA moleculesRNA can be replicatedHeat and cool cycles utilizedDNA Primers hybridize the target and initiate polymerization of new DNA MoleculesEnd product: Billions of copies of the target DNARead for presence of DNA target
38ReferencesKiser, K.M., Payne, W.C., & Taff, T.A. (2011). Clinical Laboratory Microbiology: A Practical Approach. Upper Saddle River, NJ: Pearson Education.Amjad, A., Mirza, I.A., Zia, F. (2011). Modified Hodge test: A simple and effective test for detection of carbapenemase production. Iran Journal of Microbiology. Retrieved from:New Directions in Molecular Testing: An Educational Guide for Clinical Laboratory Professionals. Gen-Probe Inc. Retrieved from: