Presentation on theme: "Microbiology Test Results: What do they mean?"— Presentation transcript:
1 Microbiology Test Results: What do they mean? Christie Massen M.S., MLS(ASCP)Microbiologist III – QA/CLIA CoordinatorNorth Dakota Department of HealthDivision of Laboratory Services - Microbiology
2 ObjectivesUpon completion of this presentation, participants will be able to:Summarize various microbiological testing methods.List the components of a laboratory report.Interpret various microbiology test results.Summarize antibiotic susceptibility testing and results.Compare various serological test results.
4 Supporting Laboratory Results ViralIdentification of virusNormal or Decreased WBC CountIncreased Lymphocyte CountParasiticIdentification of parasiteIncreased Eosinophil CountBacterialIdentification of bacteriaIncreased WBC CountIncreased Neutrophil CountLeft-shift in Neutrophil Cell LineIncreased CRPIncreased Sed-RateFungalKOH Prep TestIdentification of fungal elements
15 Modified Hodge Test Purpose: Interpretation: Detection of carbapenemase production in identified organismsInterpretation:PositiveClover leaf-like indentationalong the streak lineNegativeNo growth along the streak line
24 PCR Developed in 1985 First NAA method How it works: DNA Polymerase replicates the target DNA moleculesRNA can be replicatedHeat and cool cycles utilizedDNA Primers hybridize the target and initiate polymerization of new DNA MoleculesEnd product: Billions of copies of the target DNARead for presence of DNA target
38 ReferencesKiser, K.M., Payne, W.C., & Taff, T.A. (2011). Clinical Laboratory Microbiology: A Practical Approach. Upper Saddle River, NJ: Pearson Education.Amjad, A., Mirza, I.A., Zia, F. (2011). Modified Hodge test: A simple and effective test for detection of carbapenemase production. Iran Journal of Microbiology. Retrieved from:New Directions in Molecular Testing: An Educational Guide for Clinical Laboratory Professionals. Gen-Probe Inc. Retrieved from: