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Microbiology Test Results: What do they mean? Christie Massen M.S., MLS(ASCP) Microbiologist III – QA/CLIA Coordinator North Dakota Department of Health.

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Presentation on theme: "Microbiology Test Results: What do they mean? Christie Massen M.S., MLS(ASCP) Microbiologist III – QA/CLIA Coordinator North Dakota Department of Health."— Presentation transcript:

1 Microbiology Test Results: What do they mean? Christie Massen M.S., MLS(ASCP) Microbiologist III – QA/CLIA Coordinator North Dakota Department of Health Division of Laboratory Services - Microbiology

2 Objectives Upon completion of this presentation, participants will be able to: o Summarize various microbiological testing methods. o List the components of a laboratory report. o Interpret various microbiology test results. o Summarize antibiotic susceptibility testing and results. o Compare various serological test results.

3 Infectious Diseases Bacterial Viral Fungal Parasitic

4 Supporting Laboratory Results Bacterial o Identification of bacteria o Increased WBC Count o Increased Neutrophil Count o Left-shift in Neutrophil Cell Line o Increased CRP o Increased Sed-Rate Fungal o KOH Prep Test o Identification of fungal elements Viral o Identification of virus o Normal or Decreased WBC Count o Increased Lymphocyte Count Parasitic o Identification of parasite o Increased Eosinophil Count

5 Laboratory Reports

6 Bacterial Culture Pros o Identify the isolate o Very specific Cons o Lengthy o Normal flora Grow and isolate bacteria Various media Identify a pathogen

7 Biochemical Testing

8 Identification

9 Antibiotic Susceptibility Testing Determine antibiotic susceptibility or resistance

10 Antibiotic Susceptibility Testing

11 Case 1

12 Case 1: Report

13 Case 2

14 Case 2: Report

15 Modified Hodge Test Purpose: o Detection of carbapenemase production in identified organisms Interpretation: o Positive Clover leaf-like indentation along the streak line o Negative No growth along the streak line

16 Case 3

17 Case 3: Report

18 Virology

19 Virology Culture Cytopathic Effect (CPE)

20 Case 4

21 Case 4: Report

22 Parasitology & Mycology Direct Examination Macroscopic and Microscopic

23 Molecular Testing DNA Probes DNA sequencing o Nucleic acid sequences (16s) o Whole genome sequencing Nucleic Acid Amplification Testing (NAAT) o Polymerase Chain Reaction (PCR) o Ligase Chain Reaction (LCR) o Transcription Mediated Amplification (TMA)

24 PCR Developed in 1985 First NAA method How it works: o DNA Polymerase replicates the target DNA molecules RNA can be replicated o Heat and cool cycles utilized o DNA Primers hybridize the target and initiate polymerization of new DNA Molecules o End product: Billions of copies of the target DNA o Read for presence of DNA target

25

26 Immunology Test Methods Test Methods: o Agglutination o Nephlometry o Double Diffusion o Radial Immunodiffusion o Electrophoresis o Western Blot o Flocculation o ELISA o Immunoflorescence assay o Radioimmunoassay Detects: o Antigens o Antibodies

27 Antibody Review

28

29 Case 5

30 Case 5: Report

31 Case 6

32 Case 6: Report

33 Case 7

34 Case 7: Report

35 Case 8

36 Vaccine Preventable Disease Case 8: Report

37 Questions?

38 References Kiser, K.M., Payne, W.C., & Taff, T.A. (2011). Clinical Laboratory Microbiology: A Practical Approach. Upper Saddle River, NJ: Pearson Education. Amjad, A., Mirza, I.A., Zia, F. (2011). Modified Hodge test: A simple and effective test for detection of carbapenemase production. Iran Journal of Microbiology. Retrieved from: 0182/ New Directions in Molecular Testing: An Educational Guide for Clinical Laboratory Professionals. Gen- Probe Inc. Retrieved from:


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