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INEB - Instituto de Engenharia Biomédica Nanostructured surfaces for blood proteins selective adsorption Inês C Gonçalves 1,2, Mª Cristina L Martins 1,

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Presentation on theme: "INEB - Instituto de Engenharia Biomédica Nanostructured surfaces for blood proteins selective adsorption Inês C Gonçalves 1,2, Mª Cristina L Martins 1,"— Presentation transcript:

1 INEB - Instituto de Engenharia Biomédica Nanostructured surfaces for blood proteins selective adsorption Inês C Gonçalves 1,2, Mª Cristina L Martins 1, Mário A Barbosa 1,2 1 INEB - Instituto de Engenharia Biomédica, Lab. Biomateriais, Porto, Portugal 2 Univ. Porto, Fac. Engenharia, Dep. Eng. Metalúrgica & Materiais, Univ. Porto, Porto, Portugal 1º Encontro Ciência em Portugal – Ciência 2007 Lisboa, 12 Abril 2007

2 Blood-material interactions Biomaterial Protein Adsorption Inflammation Complement System Activation Leukocyte Adhesion and Activation Bacterial Adhesion Infection Thrombosis Platelet Adhesion and Activation Coagulation System Activation Heart valvesCathetersStents

3 Model surfaces - SAMs Self-assembled monolayers (SAMs) are well- organized organic surfaces, very useful to study the properties of the interface between a material and biological media, at the molecular scale. Form highly ordered systems Easy to prepare Wide range of functional groups to be explored XXXXX S SS S S X X- (CH2)n- SH (X = OH; COOH; CH 3 ; NH 2 ; SO 3 H; etc...) gold

4 Research purpose O=C=N-(CH 2 ) 17 CH 3 selective for albumin C18 ligand C18 SAMs (0-10%) inhibit non-specific protein adsorption EG 4 SAMs gold HS-(CH 2 ) 11 -(O-CH 2 CH 2 ) 4 -OH Develop a model surface that binds albumin in a selective and reversible way in order to decrease the adhesion and activation of platelets.

5 EG4-C18 SAMs EG 4 10% C18 SAMs Contact angle Fourier transform infrared reflection absorption spectroscopy - IRAS X-ray photoelectron spectroscopy - XPS Atomic Force Microscopy - AFM Ellipsometry

6 Selective & reversible adsorption of Albumin EG4-2.5%C18 SAMs are the only surfaces to exchange almost all the pre-adsorbed HSA by HSA in solution, but not by HFG Albumin increases with the amount of C18 ligands on the surface

7 EG42.5% C185% C1810% C18 PBS HSA Plasma Platelet adhesion & activation (original enlargement 630x)

8 INEB - Instituto de Engenharia Biomédica Future challenges… Transpose the knowledge obtained with SAMs to polymers

9 Acknowledgements FCT for my PhD grant SFRH/BD/18337/2004 FCT for the project POCTI/CTM/55644/2004 Professor Ratner from University of Washington Portuguese Blood Institute INEB


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