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Elven Automation Elves examine images and set-up data processing Elves run… mosflm scala solve mlphare dm arp/warp Holton and Alber PNAS USA 101 1537-42.

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Presentation on theme: "Elven Automation Elves examine images and set-up data processing Elves run… mosflm scala solve mlphare dm arp/warp Holton and Alber PNAS USA 101 1537-42."— Presentation transcript:

1 Elven Automation Elves examine images and set-up data processing Elves run… mosflm scala solve mlphare dm arp/warp Holton and Alber PNAS USA (2004)

2 Center for Structures of Membrane Proteins © 2006 Elven Automation Holton and Alber PNAS USA (2004) Elves examine images and set-up data processing Elves run… mosflm scala solve mlphare dm arp/warp

3 35 operating US beamlines ~10 11 ph/μm 2 exposure limit ÷ 2x10 9 ph/μm 2 /s ~ 100,000 datasets/year ÷ 1324 str in 2003 ~ 2% efficient Turning Data into Models

4 Center for Structures of Membrane Proteins © 2006 Turning Data into Models 35 operating US beamlines ~10 11 ph/μm 2 exposure limit ÷ 2x10 9 ph/μm 2 /s ~ 100,000 datasets/year ÷ 1324 str in 2003 ~ 2% efficient

5 NumberDescriptionPercent Images (~7 TB)33% 2346Data sets47% 449MAD/SAD (1:2)19% 104Published4.4% in 2003 Turning data into models

6 Center for Structures of Membrane Proteins © 2006 Turning Data into Models NumberDescriptionPercent Images (~7 TB)33% 2346Data sets47% 449MAD/SAD (1:2)19% 104Published4.4% in 2003

7 Simulated diffraction image (2008) simulatedreal

8 Center for Structures of Membrane Proteins © 2006 Simulated Diffraction Images simulatedreal

9 SimulatedstatisticReal 5.4%R merge 6.2% 18.9I/sd I/sd (1.4 Ǻ) SDCORR PADFPH mlphare f” FOM FOMDM CC(1H87) R cryst R free 0.231

10 Center for Structures of Membrane Proteins © 2006 Data processing statistics SimulatedstatisticReal 5.4%R merge 6.2% 18.9I/sd I/sd (1.4 Ǻ) SDCORR PADFPH mlphare f” FOM FOMDM CC(1H87) R cryst R free 0.231

11 mosaic spread = 0 º

12 mosaic spread = 0.1º

13 mosaic spread = 0.2º

14 mosaic spread = 0.4º

15 mosaic spread = 0.6º

16 mosaic spread = 0.8º

17 mosaic spread = 1.0º

18 mosaic spread = 1.5º

19 mosaic spread = 2.0º

20 mosaic spread = 2.5º

21 mosaic spread = 3.2º

22 mosaic spread = 6.4º

23 mosaic spread = 12.8º

24 Shutter Jitter rms timing error (% exposure) CC to correct model Bijvoet ratio = 3.8%

25 Howells et al. (2005) J. Electron. Spectrosc. Relat. Phenom. (accepted) preprint: resolution (Å) maximum tolerable dose (MGy)

26 Crystal Size crystal size (um) CC to correct model predicted Glaeser et.al. (2000) 1 μm amyloids Nelson et al Sawaya et al ~12 μm xylanase Moukhametzianov et al μm cypovirus polyhedra Coulibaly et. al μm (13x) bovine rhodopsin Standfuss et al Glaeser et.al. (2000) Silz et.al. (2003)

27 Plate goniometers

28

29 Center for Structures of Membrane Proteins © 2006


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