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MUSC Postdoctoral Retreat on the Responsible Conduct of Research “Data Management, Data Selection, and Reporting Research” Ed Krug CRI607792-1543

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Presentation on theme: "MUSC Postdoctoral Retreat on the Responsible Conduct of Research “Data Management, Data Selection, and Reporting Research” Ed Krug CRI607792-1543"— Presentation transcript:

1 MUSC Postdoctoral Retreat on the Responsible Conduct of Research “Data Management, Data Selection, and Reporting Research” Ed Krug CRI /11/09

2 Why is data management important? Document observations - don’t trust your memory!Document observations - don’t trust your memory! ReproducibilityReproducibility TroubleshootingTroubleshooting Organization of thoughtsOrganization of thoughts Maintain focusMaintain focus papers papers presentations presentations funding applications funding applications Communication with mentorCommunication with mentor Mandated by funding agency (NIH - 3 yrs post-final financial statement)Mandated by funding agency (NIH - 3 yrs post-final financial statement) May analyze data differently in the futureMay analyze data differently in the future ancillary observations ancillary observations differences in probes/reagents differences in probes/reagents alternative statistics alternative statistics

3 Essential Content for a Laboratory Notebook Bound pagesBound pages Date each experimentDate each experiment Number every page - index in frontNumber every page - index in front Relevant sections - title, purpose, materials & methods, results, conclusions, notesRelevant sections - title, purpose, materials & methods, results, conclusions, notes Initiated before conducting the experimentInitiated before conducting the experiment Include lot numbers of reagentsInclude lot numbers of reagents Cross-referenced to computer files, such as those for imagesCross-referenced to computer files, such as those for images Cross-referenced with collaborator’s notebook if joint experiment?Cross-referenced with collaborator’s notebook if joint experiment? University property - stays with PIUniversity property - stays with PI

4 Lab Notebook Layout INDEXED & PAGES NUMBERED!!INDEXED & PAGES NUMBERED!! Date and titleDate and title PurposePurpose RationaleRationale Materials and MethodsMaterials and Methods DataData CalculationsCalculations Interpretation of the dataInterpretation of the data ConclusionsConclusions Future experimentsFuture experiments Notes (discussions, sample and data storage locations, etc)Notes (discussions, sample and data storage locations, etc)

5 I strongly suggest a listing of all notebook and page numbers corresponding to any data used for a poster, manuscript or funding application! Keep this listing in a working file for the document - and give a copy to your mentor and any collaborators.

6 What does “Data Management” involve? 1) Physical record keeping lab notebook lab notebook instrument printouts instrument printouts images images computer analysis computer analysis lab meeting discussion lab meeting discussion probes and cells probes and cells 2) Analysis of primary data data selectiondata selection statistical analysisstatistical analysis calculationscalculations normalizationnormalization

7 What does “Data Management” involve? 3) Presentation of results (verification) graphsgraphs tablestables picturespictures FiguresFigures 4) Publication postersposters seminarsseminars manuscriptsmanuscripts funding requestsfunding requests

8 Image Manipulation Issues

9 “Data may be excluded from the experimental results only if you have a sound reason to do so!” Mother Nature

10 Examples of misleading presentation of the data Origin of graph not zeroOrigin of graph not zero Number of animals vs. number of determinationsNumber of animals vs. number of determinations Non-standard normalization methodNon-standard normalization method “typical results”“typical results” Not showing the entire gelNot showing the entire gel “cleaning up” the data“cleaning up” the data

11 As photographed Brightness- Contrast Adjustment

12 As photographed Erasing

13 Rossner, M. (2006). The Scientist 20: The Journal of Cell Biology: revokes the acceptance of about 1% of its papers due to inappropriate image manipulationrevokes the acceptance of about 1% of its papers due to inappropriate image manipulation 25% of accepted papers have at least 1 figure with undocumented manipulation25% of accepted papers have at least 1 figure with undocumented manipulation “If you misrepresent your data you are deceiving people.”

14 Rossner, M. and Yamada, K. (2004). J. Cell Biol. 166:11-15.

15 Rossner, M. (2006). The Scientist 20:24-25.

16 Rossner, M. and Yamada, K. (2004). J. Cell Biol. 166:11-15.

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18 What do you think?

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20 Rossner, M. and Yamada, K. (2004). J. Cell Biol. 166:11-15.

21 Nature, instructions to authors (2006) List all image acquisition and processing tools and softwareList all image acquisition and processing tools and software Document key image-gathering setting and manipulations in the Supplemental materialsDocument key image-gathering setting and manipulations in the Supplemental materials Clearly demarcate borders between images collected at different timesClearly demarcate borders between images collected at different times Avoid use of touch up tools or deliberately obscuring parts of an imageAvoid use of touch up tools or deliberately obscuring parts of an image Processing is acceptable only if applied across the entire imageProcessing is acceptable only if applied across the entire image Be prepared to deliver the original, unprocessed images to the editorBe prepared to deliver the original, unprocessed images to the editor What CAN I do?


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