Presentation on theme: "Enzyme Lab test tubes with spinach, potato, MnO2, H2O2, chalk, raw liver, cooked liver, raw hamburger, cooked hamburger."— Presentation transcript:
1Enzyme Labtest tubes with spinach, potato, MnO2, H2O2, chalk, raw liver, cooked liver, raw hamburger, cooked hamburger
2The substrate: hydrogen peroxide Hydrogen peroxide is toxic to cellsProduced as a waste product during the reactions that occur in the body.Body needs way to remove it quickly so cells don’t dieH > H2O + O2. The products water and oxygen are not harmful.Body can remove it quickly with enzyme called CATALASE. CATALASE changes H202 into water and oxygen.
3The substrate is hydrogen peroxide H2O2 It is what is being changed.It starts off as Hydrogen peroxideIt becomes Water and Oxygen
4The Enzyme is CatalaseCatalase is an enzyme found in all living things (catalase is found in spinach, potato, raw liver, cooked liver, hamburger)Catalase speeds up the breakdown of hydrogen peroxide so cells don’t die.Catalase is the same before the reaction as after. It does not change
5So what effect does heat have on enzymes? Raw liver has not had heat added to it, so it works at its best.Cooked liver has been in heat, so the enzymes that were in the liver were denatured, that means they were weakened (unraveled). Remember enzymes are proteins.
6Substrate: substance the enzyme works on Active site: where the substrate and enzyme meet
7Enzymes are very specific They are named for the substrate they work on: EX: sucrase works on sucrose
8So is MnO2 an enzyme?NO!!! MnO2 is an inorganic catalyst. That means it is man-made and it speeds up reactions.Enzymes are found in living things. EXAMPLES: catalase, protease, lipaseEnzymes can be used again and are not used up in the reaction.