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Control St Control St Control St 3 dai 7 dai 11 dai Protein extraction Tryptic digestion and TMT six-plex labeling Phosphopeptides enrichment by TiO 2.

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Presentation on theme: "Control St Control St Control St 3 dai 7 dai 11 dai Protein extraction Tryptic digestion and TMT six-plex labeling Phosphopeptides enrichment by TiO 2."— Presentation transcript:

1 Control St Control St Control St 3 dai 7 dai 11 dai Protein extraction Tryptic digestion and TMT six-plex labeling Phosphopeptides enrichment by TiO 2 Flowthrough (Total proteome) Eluate (Phosphoproteome) Biological replicate 1 Biological replicate 2 Biological replicate 3 HILIC One run Two runs One run Two runs Phosphopeptides and protein identification and validation Bioinformatics analysis One run Three biological replicates LC- MS/MS Fig. S1

2 Stakado Sevin A B 3 dai 7 dai 11 dai Stakado Sevin Fig. S2

3 Fig. S3

4 * * * * * * * * * * * * * * Fig. S4


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