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WP8: Analysis of antioxidants (tocols) CRA-MAC Nicola Berardo Sabrina Locatelli Lorenzo Guerrini Rita Redaelli AVEQ final Meeting – Bucarest, 18-22 October.

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Presentation on theme: "WP8: Analysis of antioxidants (tocols) CRA-MAC Nicola Berardo Sabrina Locatelli Lorenzo Guerrini Rita Redaelli AVEQ final Meeting – Bucarest, 18-22 October."— Presentation transcript:

1 WP8: Analysis of antioxidants (tocols) CRA-MAC Nicola Berardo Sabrina Locatelli Lorenzo Guerrini Rita Redaelli AVEQ final Meeting – Bucarest, October 2010

2 Method for analysis Reducing Time Analysis for the Determination of Tocols in Cereals by Normal-Phase High Performance Liquid Chromatography V. Pisacane, R. Redaelli and N. Berardo J. Genet. & Breed. 58: (2004)

3 AVEQ final Meeting – Bucarest, October 2010 STANDARD MIX OF TOCOLS - CHROMATOGRAM

4 AVEQ final Meeting – Bucarest, October 2010 TOCOLS IN A SAMPLE - CHROMATOGRAM

5 AVEQ final Meeting – Bucarest, October 2010 Tocopherolsα-Tβ-Tγ-Tδ-TTT Mean Min Max SD BULGARIA - STANDARD CULTIVARS Tocotrienolsα-T3β-T3γ-T3δ-T3TT3Tocols Mean Min Max SD

6 AVEQ final Meeting – Bucarest, October 2010 α-Tβ-Tγ-Tδ-TTT Mean Min Max SD BULGARIA – SELECTED ACCESSIONS α-T3β-T3γ-T3δ-T3TT3Tocols Mean Min Max SD

7 AVEQ final Meeting – Bucarest, October 2010

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10 Tocopherolsα-Tβ-Tγ-Tδ-TTT Mean Min Max SD ESTONIA - STANDARD CULTIVARS Tocotrienolsα-T3β-T3γ-T3δ-T3TT3Tocols Mean Min Max SD

11 AVEQ final Meeting – Bucarest, October 2010 Tocopherolsα-Tβ-Tγ-Tδ-TTT Mean Min Max SD ESTONIA – SELECTED ACCESSIONS Tocotrienolsα-T3β-T3γ-T3δ-T3TT3Tocols Mean Min Max SD

12 AVEQ final Meeting – Bucarest, October 2010

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15 STANDARD CULTIVARS – ESTONIA E BULGARIA 2008

16 TAXAEstoniaBulgaria A. abyssinica A. barbata subsp. barbata A. byzantina A. byzantina subsp. byzantina A. sativa A. sativa subsp. nudisativa A. sativa subsp. sativa var. aristata A. sativa subsp. sativa var. aurea A. sativa subsp. sativa var. brunnea A. sativa subsp. sativa var. cinerea A. sativa subsp. sativa var. grisea A. sativa subsp. sativa var. iranica A. sativa subsp. sativa var. montana A. sativa subsp. sativa var. mutica A. sativa subsp. sativa var. obtusata A. sativa subsp. sativa var. persica A. sativa subsp. sativa var. tristis A. sativa var. brachytricha A. sterilis subsp. sterilis A. strigosa A. strigosa subsp. brevis var. nigricans A. strigosa var. typica Total tocols content mg kg -1 dm


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