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WP8: Analysis of antioxidants (tocols) CRA-MAC Nicola Berardo Sabrina Locatelli Lorenzo Guerrini Rita Redaelli AVEQ final Meeting – Bucarest, 18-22 October.

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Presentation on theme: "WP8: Analysis of antioxidants (tocols) CRA-MAC Nicola Berardo Sabrina Locatelli Lorenzo Guerrini Rita Redaelli AVEQ final Meeting – Bucarest, 18-22 October."— Presentation transcript:

1 WP8: Analysis of antioxidants (tocols) CRA-MAC Nicola Berardo Sabrina Locatelli Lorenzo Guerrini Rita Redaelli AVEQ final Meeting – Bucarest, 18-22 October 2010

2 Method for analysis Reducing Time Analysis for the Determination of Tocols in Cereals by Normal-Phase High Performance Liquid Chromatography V. Pisacane, R. Redaelli and N. Berardo J. Genet. & Breed. 58: 253-258 (2004)

3 AVEQ final Meeting – Bucarest, 18-22 October 2010 STANDARD MIX OF TOCOLS - CHROMATOGRAM

4 AVEQ final Meeting – Bucarest, 18-22 October 2010 TOCOLS IN A SAMPLE - CHROMATOGRAM

5 AVEQ final Meeting – Bucarest, 18-22 October 2010 Tocopherolsα-Tβ-Tγ-Tδ-TTT Mean 12.272.140.190.1214.73 Min 8.061.160.00 9.40 Max 18.603.931.141.0121.09 SD 2.530.680.250.222.76 BULGARIA - STANDARD CULTIVARS Tocotrienolsα-T3β-T3γ-T3δ-T3TT3Tocols Mean 24.645.341.601.2632.6746.95 Min 14.573.460.00 18.8920.94 Max 49.8311.793.672.3365.7485.43 SD 7.431.780.820.598.6010.90

6 AVEQ final Meeting – Bucarest, 18-22 October 2010 α-Tβ-Tγ-Tδ-TTT Mean 23.831.671.570.3627.43 Min 7.660.00 10.17 Max 50.305.615.761.6751.99 SD 9.971.372.210.4310.13 BULGARIA – SELECTED ACCESSIONS α-T3β-T3γ-T3δ-T3TT3Tocols Mean 45.273.290.270.3349.0775.48 Min 22.810.020.00 27.5939.68 Max 102.7612.862.301.38107.85159.84 SD 16.732.530.390.3517.5622.24

7 AVEQ final Meeting – Bucarest, 18-22 October 2010

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10 Tocopherolsα-Tβ-Tγ-Tδ-TTT Mean 8.172.460.04 10.71 Min 5.801.100.00 7.42 Max 12.635.230.30 15.53 SD 1.431.000.080.061.91 ESTONIA - STANDARD CULTIVARS Tocotrienolsα-T3β-T3γ-T3δ-T3TT3Tocols Mean 12.855.851.060.9520.5630.92 Min 7.023.670.00 9.3710.83 Max 32.268.342.942.0941.2753.88 SD 4.201.380.540.675.076.94

11 AVEQ final Meeting – Bucarest, 18-22 October 2010 Tocopherolsα-Tβ-Tγ-Tδ-TTT Mean 28.592.080.060.0730.80 Min 5.640.780.00 7.61 Max 65.174.490.560.8668.60 SD 18.130.660.130.1218.02 ESTONIA – SELECTED ACCESSIONS Tocotrienolsα-T3β-T3γ-T3δ-T3TT3Tocols Mean 19.975.400.790.2926.4557.25 Min 4.423.090.00 8.9126.57 Max 58.1212.132.401.6062.29116.50 SD 9.341.410.660.329.5920.86

12 AVEQ final Meeting – Bucarest, 18-22 October 2010

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15 STANDARD CULTIVARS – ESTONIA E BULGARIA 2008

16 TAXAEstoniaBulgaria A. abyssinica41.7662.88 A. barbata subsp. barbata116.5084.67 A. byzantina41.6664.81 A. byzantina subsp. byzantina56.6868.16 A. sativa53.0272.70 A. sativa subsp. nudisativa47.1978.47 A. sativa subsp. sativa var. aristata46.8770.64 A. sativa subsp. sativa var. aurea64.4768.75 A. sativa subsp. sativa var. brunnea55.8059.09 A. sativa subsp. sativa var. cinerea37.2777.40 A. sativa subsp. sativa var. grisea88.0868.68 A. sativa subsp. sativa var. iranica33.6465.05 A. sativa subsp. sativa var. montana72.10110.82 A. sativa subsp. sativa var. mutica56.9670.01 A. sativa subsp. sativa var. obtusata46.6948.68 A. sativa subsp. sativa var. persica42.5768.80 A. sativa subsp. sativa var. tristis54.0184.44 A. sativa var. brachytricha67.4639.68 A. sterilis subsp. sterilis45.8490.20 A. strigosa81.78110.73 A. strigosa subsp. brevis var. nigricans43.95159.84 A. strigosa var. typica68.1599.50 Total tocols content mg kg -1 dm


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