1. Microscopy Cell Structure Mitosis
A&P 1 - Lab #2
Microscopy
Cell Structure
Mitosis

2. Arm Stage Condenser Illuminator Base Objective Lens Ocular Lens
Rotatable Head
Arm
Mechanical Stage
Objective Lens
Mechanical Stage Control
Stage
Course Adjustment Knob
Condenser
Fine Adjustment Knob
Illuminator
Base

3. Magnification
Total magnification is achieved by multiplying the power of the ocular lens by the power of the objective lens
Total Magnification = Ocular Lens X Objective Lens

4. Objective Lens Red Band = Low Power = 4
4(obj lens) X 10(ocular lens) = 40X
Yellow Band = Medium Power = 10
10(obj lens) X 10(ocular lens) = 100X
Blue Band = High Power = 100
40(obj lens) X 10(ocular lens) = 400X
White Band = Oil Immersion

5. Follow the Rules! ALWAYS begin on LOW POWER
Once you have the specimen in focus
Move to medium power
Refocus, then you can move to high power
Once you move off low power, you can NEVER, NEVER, NEVER use the coarse adjustment

6. Orientation of Specimen

7. Specimen is flipped horizontally & vertically

8. Depth of Field

9. Preparing a Wet Mount
Slowly Lower
Cover Slip
Cover Slip 45O

10. Air Bubbles - Try Again!

11. Types of Cells There are 2 types of cells: Prokaryotic Eukaryotic
(pro- before) (eu- true)
-kary = nucleus

12. Prokaryotic Cells

13. Prokaryotic Cells ALL bacteria are prokaryotic Prokaryotic cells
-lack a membrane-bound nucleus.
-genetic material is present in the nucleoid
ALL prokaryotic cells are bacteria, and
ALL bacteria are prokaryotic
The only place you will find prokaryotic cells in is the KINGDOM BACTERIA AND ARCHEBACTERIA

14. Eukaryotic Cells

15. Epithelial - Cheek Cells

16. The Cell Cycle Interphase Mitosis Cytokinesis Prophase - prepare
Metaphase - middle
Anaphase - apart
Telophase - two
Cytokinesis
cyto- = cell kinesis = to move

17. The Cell Cycle

18. Interphase

19. Mitosis - Prophase

20. Mitosis - Metaphase

21. Mitosis - Metaphase

22. Mitosis - Anaphase

23. Mitosis - Anaphase

24. Mitosis - Telophase

25. Mitosis - Telophase/Cytokinesis

26. Practice Identifying the Stages of Mitosis
Select the link below (you must be in “slide show” mode)
If you are unable to view a slide show, you can go to
Bookmarks on Ocean Cruiser. The link is found there.
Mitosis