1. In The Name Of God
Precipitation

2. Antigen/Antibody Reaction
Primary reaction :
Immunoassay :Like Elisa , RIA , IFA
Secondary reaction :
Lattice formation like Agglutination , Hemagglutination
Complement Fixation, Neutralization ,
Precipitation : soluble antibody reacts with soluble antigen
Tertiary reaction :
Detected by affect on tissues or cells.

3. Secondary Phenomenon

4. precipitation : proteins Several types: Protein precipitation SRID DID
ImmunoElectherophoresis:
Counter current IEP
Rocket IEP

5. Single Radial Immuno diffusion or Mancini
SRID:
Single Radial Immuno diffusion or Mancini
SRID plate preparation:
(Agarose ِ+ BUFFER (
Ab (Anti IgG )
Punch
Sampling
Incubation in RT for 24h
measuring of diameter
making of curve
Calculation of sample concentration

6. SRID Plate

9. Single Radial Immuno diffusion : In SRID :
34 GAM = Is useful for measuring of IgG , IgA, IgM,C3, C4
Gel is mixed with Ab
Ag is in punch
In Single Radial Diffusion: one component is fixed
(e.g. antigen-in this case it is IgG)
As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed as
illustrated in Figure
The diameter of the ring is proportional to the concentration of Ag .

11. (Double Immuno Diffusion)= DID or Ouchterlony Immunodiffusion
Qualitative
2 Diffusion
Application in research laboratory
Is not as a routin method now

14. Ouchterlony - Identity

15. Ouchterlony – Partial Identity

16. Ouchterlony Non-Identity

17. measuring the amount of light Passing Through the solution.
Turbidimetery
measuring the amount of light Passing Through the solution.

18. Measures SCATTERED light
Nephelometery
Measures SCATTERED light

19. Immunoelectrophoresis
Counter current IEP : Qualitative
Rocket IEP: Quantitative

20. Thanks For your Attention