4 precipitation : proteins Several types: Protein precipitation SRID DID ImmunoElectherophoresis:Counter current IEPRocket IEP
5 Single Radial Immuno diffusion or Mancini SRID:Single Radial Immuno diffusion or ManciniSRID plate preparation:(Agarose ِ+ BUFFER (Ab (Anti IgG )PunchSamplingIncubation in RT for 24hmeasuring of diametermaking of curveCalculation of sample concentration
9 Single Radial Immuno diffusion : In SRID : 34 GAM = Is useful for measuring of IgG , IgA, IgM,C3, C4Gel is mixed with AbAg is in punchIn Single Radial Diffusion: one component is fixed(e.g. antigen-in this case it is IgG)As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed asillustrated in FigureThe diameter of the ring is proportional to the concentration of Ag .
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