Presentation on theme: "In The Name Of God Precipitation. Antigen/Antibody Reaction Primary reaction : Immunoassay :Like Elisa, RIA, IFA Secondary reaction : Lattice formation."— Presentation transcript:
In The Name Of God Precipitation
Antigen/Antibody Reaction Primary reaction : Immunoassay :Like Elisa, RIA, IFA Secondary reaction : Lattice formation like Agglutination, Hemagglutination Complement Fixation, Neutralization, Precipitation : soluble antibody reacts with soluble antigen Tertiary reaction : Detected by affect on tissues or cells.
precipitation : proteins Several types: Protein precipitation SRID DID ImmunoElectherophoresis: Counter current IEP Rocket IEP
SRID plate preparation: (Agarose ِ + BUFFER ( Ab (Anti IgG ) Punch Sampling Incubation in RT for 24h measuring of diameter making of curve Calculation of sample concentration SRID: Single Radial Immuno diffusion or Mancini
Single Radial Immuno diffusion : In SRID : 34 GAM = Is useful for measuring of IgG, IgA, IgM,C3, C4 Gel is mixed with Ab Ag is in punch In Single Radial Diffusion: one component is fixed (e.g. antigen-in this case it is IgG) As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed as illustrated in Figure The diameter of the ring is proportional to the concentration of Ag.
(Double Immuno Diffusion)= DID or Ouchterlony Immunodiffusion Qualitative 2 Diffusion Application in research laboratory Is not as a routin method now
Ouchterlony - Identity
Ouchterlony – Partial Identity
Turbidimetery measuring the amount of light Passing Through the solution.
Nephelometery Measures SCATTERED light
Immunoelectrophoresis Counter current IEP : Qualitative Rocket IEP: Quantitative