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In The Name Of God Precipitation. Antigen/Antibody Reaction Primary reaction : Immunoassay :Like Elisa, RIA, IFA Secondary reaction : Lattice formation.

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Presentation on theme: "In The Name Of God Precipitation. Antigen/Antibody Reaction Primary reaction : Immunoassay :Like Elisa, RIA, IFA Secondary reaction : Lattice formation."— Presentation transcript:

1 In The Name Of God Precipitation

2 Antigen/Antibody Reaction Primary reaction : Immunoassay :Like Elisa, RIA, IFA Secondary reaction : Lattice formation like Agglutination, Hemagglutination Complement Fixation, Neutralization, Precipitation : soluble antibody reacts with soluble antigen Tertiary reaction : Detected by affect on tissues or cells.

3 Secondary Phenomenon

4 precipitation : proteins Several types: Protein precipitation SRID DID ImmunoElectherophoresis: Counter current IEP Rocket IEP

5 SRID plate preparation: (Agarose ِ + BUFFER ( Ab (Anti IgG ) Punch Sampling Incubation in RT for 24h measuring of diameter making of curve Calculation of sample concentration SRID: Single Radial Immuno diffusion or Mancini

6 SRID Plate

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9 Single Radial Immuno diffusion : In SRID : 34 GAM = Is useful for measuring of IgG, IgA, IgM,C3, C4 Gel is mixed with Ab Ag is in punch In Single Radial Diffusion: one component is fixed (e.g. antigen-in this case it is IgG) As the Ag diffuses into the gel it reacts with the Ab and when the equivalence point is reached a ring of precipitation is formed as illustrated in Figure The diameter of the ring is proportional to the concentration of Ag.

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11 (Double Immuno Diffusion)= DID or Ouchterlony Immunodiffusion Qualitative 2 Diffusion Application in research laboratory Is not as a routin method now

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14 Ouchterlony - Identity

15 Ouchterlony – Partial Identity

16 Ouchterlony Non-Identity

17 Turbidimetery measuring the amount of light Passing Through the solution.

18 Nephelometery Measures SCATTERED light

19 Immunoelectrophoresis Counter current IEP : Qualitative Rocket IEP: Quantitative

20 Thanks For your Attention


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