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Him-3 High Incident Males (increased X chromosome loss) By Chang Lee.

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Presentation on theme: "Him-3 High Incident Males (increased X chromosome loss) By Chang Lee."— Presentation transcript:

1 him-3 High Incident Males (increased X chromosome loss) By Chang Lee

2 him-3 encodes a homolog of the S. cerevisiae HOP1 protein which is required for the formation of the synaptonemal complex during meiosis Predicted by GeneFinder Washington University, St. Louis MO.

3 How large? 1.2 kb mRNA sequence : 1113 bp 4 exons The gene contains 3 introns

4 What chromosome is the gene on? chromosome IV positions 6,977,403 to 6,978,631

5 There are available cDNA CK575867, EB994223, OSTF002C4_1 EC023874 CK589379 AF172351 EST data

6 Mutants Mutant e1147 gk149 vv6 e1256 me80 Its phenotype is embryonic lethal, partial, high incidence of males, increased X chromosome loss

7 The final gene product is a HOP1- like protein him-3 encodes a homolog of the S. cerevisiae HOP1 protein

8 homologs in other species C. Briggsae 4.5e-66 C remanei 1.4e-27 C Elegans 2e-22 M Musculus 3.6e-12 H sapiens 4.8-12

9 structural components The DNA-binding HORMA motif is seen in the product of this geneDNA-binding HORMA The HORMA (for Hop1p, Rev7p and MAD2) domain has been suggested

10 molecular function HIM-3 may be part of a mechanistic link that exists between the early prophase events of synapsis and recombination, and later events such as segregation

11 references http://www.genesdev.org/cgi/search?session_query_re f=rbs.queryref_1209325456816&COLLECTIONS=hw1& JC=genesdev&FULLTEXT=%28size+AND+of+AND+him - 3+AND+gene%29&FULLTEXTFIELD=lemcontent&RESO URCETYPE=HWCIT&ABSTRACTFIELD=lemhwcompabstr act&TITLEFIELD=lemhwcomptitlehttp://www.genesdev.org/cgi/search?session_query_re f=rbs.queryref_1209325456816&COLLECTIONS=hw1& JC=genesdev&FULLTEXT=%28size+AND+of+AND+him - 3+AND+gene%29&FULLTEXTFIELD=lemcontent&RESO URCETYPE=HWCIT&ABSTRACTFIELD=lemhwcompabstr act&TITLEFIELD=lemhwcomptitle http://www.wormbase.org/db/gene/gene?name=WBGe ne00001862;class=Genehttp://www.wormbase.org/db/gene/gene?name=WBGe ne00001862;class=Gene http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/a v.cgi?db=worm&q=4H743http://www.ncbi.nlm.nih.gov/IEB/Research/Acembly/a v.cgi?db=worm&q=4H743 http://linkinghub.elsevier.com/retrieve/pii/S096098220 4002179http://linkinghub.elsevier.com/retrieve/pii/S096098220 4002179 http://www.sanger.ac.uk/cgi-bin/Pfam/getacc?PF02301


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