Timeline Day 1 Introduce DNA fingerprinting RE and electrophoresis activity Day 2 DNA fingerprinting Worksheet DNA fingerprinting video Students will observe gel and determine guilty suspect
DNA DNA makes you unique. Your DNA is different from every other person in the world. An individual’s DNA forms a unique pattern of bands that can be used to identify the person. This unique banding pattern produced by fragments of your DNA is called DNA fingerprinting. Law enforcement is able to gather the smallest amount of evidence at a crime scene and compare it to DNA from a suspect’s blood or saliva.
Day 1: Restriction Enzymes and electrophoresis Introduce restriction enzymes and electrophoresis (PPT presentation) Activity (Restriction Enzymes and electrophoresis Worksheet)
Restriction Enzymes and electrophoresis
Day 2: Crime Scene Introduce students to crime scene scenario Class demonstration Load gels with suspect and crime scene DNA (FisherScientific #S97676) Simplify lab by using dye samples (kit or make own) During gel electrophoresis:DNA fingerprinting video.DNA fingerprinting video Analyze data
Alternative NOVA Fingerprinting
High School Modification Students can make their own gels and prepare their own DNA Explain electrophoresis and restriction enzymes in more detail Change crime scene scenario