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GK-12 Saturday Workshop RS: Roxanna Ochoa CT: Charie Poderoso October 20, 2012.

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Presentation on theme: "GK-12 Saturday Workshop RS: Roxanna Ochoa CT: Charie Poderoso October 20, 2012."— Presentation transcript:

1 GK-12 Saturday Workshop RS: Roxanna Ochoa CT: Charie Poderoso October 20, 2012

2 In this lab, students will be introduced to restriction enzymes, DNA profiling and gel electrophoresis

3 Standards Addressed Cell Biology 1a, 1c Genetics 2d, 2e Investigation & Experimentation 7a, 7c

4 Timeline Day 1 Introduce DNA fingerprinting RE and electrophoresis activity Day 2 DNA fingerprinting Worksheet DNA fingerprinting video Students will observe gel and determine guilty suspect

5 DNA DNA makes you unique. Your DNA is different from every other person in the world. An individual’s DNA forms a unique pattern of bands that can be used to identify the person. This unique banding pattern produced by fragments of your DNA is called DNA fingerprinting. Law enforcement is able to gather the smallest amount of evidence at a crime scene and compare it to DNA from a suspect’s blood or saliva.

6 Day 1: Restriction Enzymes and electrophoresis Introduce restriction enzymes and electrophoresis (PPT presentation) Activity (Restriction Enzymes and electrophoresis Worksheet)

7 Restriction Enzymes and electrophoresis

8 Day 2: Crime Scene Introduce students to crime scene scenario Class demonstration Load gels with suspect and crime scene DNA (FisherScientific #S97676) Simplify lab by using dye samples (kit or make own) During gel electrophoresis:DNA fingerprinting video.DNA fingerprinting video Analyze data

9 Lab Worksheet

10 Alternative NOVA Fingerprinting

11 High School Modification Students can make their own gels and prepare their own DNA Explain electrophoresis and restriction enzymes in more detail Change crime scene scenario


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